Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GALF_RS13370 Genome accession   NC_014394
Coordinates   2886900..2887943 (+) Length   347 a.a.
NCBI ID   WP_013294597.1    Uniprot ID   D9SD80
Organism   Gallionella capsiferriformans ES-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2881900..2892943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GALF_RS13340 (Galf_2676) rng 2882013..2883467 (+) 1455 WP_013294591.1 ribonuclease G -
  GALF_RS13345 (Galf_2677) - 2883560..2884402 (+) 843 WP_013294592.1 delta-class carbonic anhydrase -
  GALF_RS13350 (Galf_2678) - 2884450..2884740 (-) 291 WP_013294593.1 DUF167 domain-containing protein -
  GALF_RS13355 (Galf_2679) - 2884740..2885306 (-) 567 WP_013294594.1 YggT family protein -
  GALF_RS13360 (Galf_2680) proC 2885303..2886115 (-) 813 WP_013294595.1 pyrroline-5-carboxylate reductase -
  GALF_RS13365 (Galf_2681) - 2886141..2886836 (-) 696 WP_013294596.1 YggS family pyridoxal phosphate-dependent enzyme -
  GALF_RS13370 (Galf_2682) pilT 2886900..2887943 (+) 1044 WP_013294597.1 type IV pilus twitching motility protein PilT Machinery gene
  GALF_RS13375 (Galf_2683) pilU 2888010..2889146 (+) 1137 WP_013294598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GALF_RS13380 (Galf_2684) - 2889174..2889584 (+) 411 WP_013294599.1 CoA-binding protein -
  GALF_RS13385 (Galf_2685) - 2889581..2890156 (+) 576 WP_013294600.1 DNA-3-methyladenine glycosylase I -
  GALF_RS13390 (Galf_2686) dapF 2890158..2890988 (+) 831 WP_013294601.1 diaminopimelate epimerase -
  GALF_RS13395 (Galf_2687) - 2890998..2891624 (+) 627 WP_013294602.1 DUF484 family protein -
  GALF_RS13400 (Galf_2688) xerC 2891584..2892537 (+) 954 WP_013294603.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38487.54 Da        Isoelectric Point: 7.2101

>NTDB_id=38111 GALF_RS13370 WP_013294597.1 2886900..2887943(+) (pilT) [Gallionella capsiferriformans ES-2]
MDITELLAFGVKNKASDLHLSSGLPPMIRVHGDMRRINLPAMEHKQVHAMIYDIMNDGQRKFYEENKEVDFSFEIPNLAR
FRVNAFIQQRGAAAVLRTIPSKVFSLEDLNAPKIFTDISSFPRGLVLVTGPTGSGKSTTLAAMVNYINEKEQGHILTVED
PIEFVHESKKCLINQREVGRDTLSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQALLKTKDGTGRVAAHEIMMCTPAIRNLIREAKVPQMYSAIQTGQGMGMQTLDQC
LTNLVKSNMISPAEARTKAMNKDMFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=38111 GALF_RS13370 WP_013294597.1 2886900..2887943(+) (pilT) [Gallionella capsiferriformans ES-2]
ATGGATATCACCGAACTGCTTGCCTTCGGCGTCAAGAACAAAGCCTCCGACCTGCACCTGTCCTCCGGACTACCACCGAT
GATACGTGTACACGGCGATATGCGCCGCATCAATTTGCCCGCGATGGAACATAAACAGGTTCACGCGATGATTTACGACA
TTATGAACGACGGGCAGCGAAAGTTTTACGAAGAGAATAAGGAAGTCGACTTTTCCTTCGAAATCCCTAATCTGGCGCGT
TTCCGCGTCAACGCCTTCATCCAGCAACGCGGCGCGGCGGCCGTACTGCGAACCATTCCTTCCAAGGTATTCAGCCTCGA
GGACTTGAATGCGCCGAAAATTTTCACCGATATTTCCAGTTTTCCCCGCGGCCTGGTGCTGGTCACAGGCCCGACTGGGT
CGGGAAAATCTACCACCCTAGCCGCGATGGTCAATTACATCAATGAAAAGGAACAAGGTCACATTCTGACTGTGGAAGAT
CCAATCGAATTCGTGCACGAATCGAAAAAGTGCCTGATCAATCAGCGCGAAGTCGGACGCGATACGCTGTCCTTCAATGC
GGCGCTGCGCTCGGCACTGCGCGAAGATCCCGATGTGATTCTGGTCGGTGAAATGCGCGATCTGGAAACCATACGTCTGG
CGATGACCGCGGCGGAAACCGGCCATCTGGTATTCGGCACCCTGCACACCAGTTCGGCGGCCAAAACAATCGACCGTATC
ATCGACGTTTTTCCGGCCGATGAAAAAGAAATGGTGCGCGCGATGCTGTCCGAAAGCTTGCGCGCGGTGATTTCACAGGC
GCTGCTCAAAACCAAGGACGGCACAGGCCGCGTGGCCGCACATGAAATCATGATGTGTACCCCCGCGATCCGCAACCTGA
TACGCGAGGCCAAAGTGCCGCAGATGTACTCCGCGATTCAGACCGGACAAGGCATGGGTATGCAGACGCTGGATCAATGC
CTGACCAATCTGGTGAAAAGCAATATGATTTCACCCGCTGAAGCGCGCACCAAAGCAATGAACAAGGATATGTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D9SD80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Legionella pneumophila strain Lp02

75.072

99.424

0.746
  pilT Legionella pneumophila strain ERS1305867

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Pseudomonas aeruginosa PAK

75.221

97.695

0.735
  pilT Neisseria meningitidis 8013

71.72

98.847

0.709
  pilT Neisseria gonorrhoeae MS11

71.429

98.847

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.047

97.406

0.663
  pilT Vibrio cholerae strain A1552

68.047

97.406

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

40.588

97.983

0.398

Multiple sequence alignment