Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GALF_RS13375 Genome accession   NC_014394
Coordinates   2888010..2889146 (+) Length   378 a.a.
NCBI ID   WP_013294598.1    Uniprot ID   D9SD81
Organism   Gallionella capsiferriformans ES-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2883010..2894146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GALF_RS13345 (Galf_2677) - 2883560..2884402 (+) 843 WP_013294592.1 delta-class carbonic anhydrase -
  GALF_RS13350 (Galf_2678) - 2884450..2884740 (-) 291 WP_013294593.1 DUF167 domain-containing protein -
  GALF_RS13355 (Galf_2679) - 2884740..2885306 (-) 567 WP_013294594.1 YggT family protein -
  GALF_RS13360 (Galf_2680) proC 2885303..2886115 (-) 813 WP_013294595.1 pyrroline-5-carboxylate reductase -
  GALF_RS13365 (Galf_2681) - 2886141..2886836 (-) 696 WP_013294596.1 YggS family pyridoxal phosphate-dependent enzyme -
  GALF_RS13370 (Galf_2682) pilT 2886900..2887943 (+) 1044 WP_013294597.1 type IV pilus twitching motility protein PilT Machinery gene
  GALF_RS13375 (Galf_2683) pilU 2888010..2889146 (+) 1137 WP_013294598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GALF_RS13380 (Galf_2684) - 2889174..2889584 (+) 411 WP_013294599.1 CoA-binding protein -
  GALF_RS13385 (Galf_2685) - 2889581..2890156 (+) 576 WP_013294600.1 DNA-3-methyladenine glycosylase I -
  GALF_RS13390 (Galf_2686) dapF 2890158..2890988 (+) 831 WP_013294601.1 diaminopimelate epimerase -
  GALF_RS13395 (Galf_2687) - 2890998..2891624 (+) 627 WP_013294602.1 DUF484 family protein -
  GALF_RS13400 (Galf_2688) xerC 2891584..2892537 (+) 954 WP_013294603.1 tyrosine recombinase XerC -
  GALF_RS13405 (Galf_2689) - 2892534..2893871 (+) 1338 WP_013294604.1 class I SAM-dependent rRNA methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42158.34 Da        Isoelectric Point: 6.7857

>NTDB_id=38112 GALF_RS13375 WP_013294598.1 2888010..2889146(+) (pilU) [Gallionella capsiferriformans ES-2]
MERDQATELIHNLLRGMASKKASDLFITAGFPPAFKVDGKMTPVSSQPLSHQHTRELARSIMNDRQTAEFEASHECNFAI
SPHGIGRFRVNVFMQQQHVGMVMRTITTKIPNLDEMGMPDILKDIVMTKRGLVILVGATGSGKSTTLAGMLGHRNQNSFG
HIITIEDPVEYVHEHGNCIVTHREVGVDTESWQAALKNTLRQAPDVILIGEIRDRETMEYAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERREQLLMDLSLNVKALISQRLIPKKDGSGRSAAMEILLNSPLISDLIFKGDVHAIKGVMAKSRELG
MQTFDQALFDLYEAHAISYEEALKNADSVNDLRLKIKLESRHTADRDVMSGTHHLQMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=38112 GALF_RS13375 WP_013294598.1 2888010..2889146(+) (pilU) [Gallionella capsiferriformans ES-2]
ATGGAAAGAGATCAGGCCACCGAGCTGATACACAATTTGTTGCGCGGCATGGCCAGCAAGAAAGCATCTGACTTATTTAT
CACCGCAGGCTTTCCGCCCGCATTCAAGGTCGATGGCAAGATGACCCCGGTTTCCAGCCAGCCGCTGTCGCATCAGCATA
CCCGCGAACTGGCGCGCAGCATCATGAACGACCGCCAAACCGCCGAGTTCGAAGCCTCACACGAGTGCAATTTCGCGATC
AGCCCGCACGGCATCGGCCGCTTCCGCGTCAACGTATTTATGCAACAGCAGCATGTCGGCATGGTCATGCGCACCATCAC
CACCAAAATACCCAATCTCGACGAGATGGGCATGCCCGACATCCTCAAAGACATCGTGATGACCAAGCGCGGTCTGGTCA
TTCTGGTGGGCGCGACCGGTTCGGGCAAATCCACTACGCTGGCGGGCATGCTGGGCCATCGTAACCAGAACAGCTTTGGA
CACATCATCACGATCGAAGATCCGGTCGAATATGTGCATGAACATGGCAACTGCATCGTCACGCACCGCGAAGTCGGGGT
GGATACGGAATCCTGGCAGGCAGCGCTGAAAAATACGCTGCGTCAGGCACCCGATGTGATTCTGATCGGCGAAATTCGCG
ACCGGGAAACCATGGAATATGCGGTCGCCTTTGCCGAAACAGGCCATTTGTGCATGGCAACCTTGCACGCGAACAGCGCC
AATCAGGCGCTCGATCGCATCATCAACTTCTTTCCCGAAGAGCGTCGCGAACAGTTGTTGATGGACCTGTCGCTCAACGT
CAAGGCGCTCATCTCGCAACGCCTCATCCCCAAAAAAGATGGTTCAGGCCGATCCGCCGCGATGGAAATCCTGCTCAACT
CGCCGCTGATCTCCGATCTGATCTTCAAAGGCGACGTTCATGCGATCAAAGGCGTGATGGCCAAATCCCGCGAACTGGGT
ATGCAGACGTTCGATCAGGCGCTGTTTGATTTGTATGAGGCGCATGCCATCAGCTACGAAGAAGCACTCAAAAATGCAGA
CTCGGTCAACGACCTGCGCCTCAAAATCAAACTGGAGAGCCGCCACACCGCCGACCGCGATGTAATGAGCGGCACCCATC
ACCTGCAAATGACTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D9SD81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.506

92.063

0.585
  pilU Acinetobacter baylyi ADP1

60.571

92.593

0.561
  pilU Vibrio cholerae strain A1552

56.034

92.063

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.478

89.153

0.423
  pilT Pseudomonas aeruginosa PAK

43.62

89.153

0.389
  pilT Acinetobacter baylyi ADP1

42.183

89.683

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter baumannii strain A118

41.742

88.095

0.368
  pilT Acinetobacter nosocomialis M2

41.742

88.095

0.368
  pilT Acinetobacter baumannii D1279779

41.742

88.095

0.368
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365

Multiple sequence alignment