Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FVF62_RS00215 Genome accession   NZ_CP042931
Coordinates   33638..34675 (+) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain ABCR01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 14954..56488 33638..34675 within 0


Gene organization within MGE regions


Location: 14954..56488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVF62_RS00140 (FVF62_00140) - 14954..16111 (-) 1158 WP_000947475.1 tyrosine-type recombinase/integrase -
  FVF62_RS00150 (FVF62_00150) ggt 16809..18794 (-) 1986 WP_001042923.1 gamma-glutamyltransferase -
  FVF62_RS00155 (FVF62_00155) - 19097..20620 (-) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  FVF62_RS00160 (FVF62_00160) - 20627..21778 (-) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  FVF62_RS00165 (FVF62_00165) - 22048..22545 (-) 498 WP_002019577.1 thioesterase family protein -
  FVF62_RS00170 (FVF62_00170) hemB 22688..23701 (+) 1014 WP_034118559.1 porphobilinogen synthase -
  FVF62_RS00175 (FVF62_00175) - 23727..24842 (+) 1116 WP_000546701.1 NAD(P)/FAD-dependent oxidoreductase -
  FVF62_RS00180 (FVF62_00180) - 24834..25739 (-) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  FVF62_RS00185 (FVF62_00185) - 25829..26281 (-) 453 WP_000164153.1 ABZJ_00895 family protein -
  FVF62_RS00190 (FVF62_00190) - 26661..27038 (+) 378 WP_001059438.1 VOC family protein -
  FVF62_RS00195 (FVF62_00195) - 27106..27579 (-) 474 WP_000974358.1 OsmC family protein -
  FVF62_RS00200 (FVF62_00200) - 27743..29011 (+) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  FVF62_RS00205 (FVF62_00205) - 29021..32617 (+) 3597 WP_000698785.1 AAA family ATPase -
  FVF62_RS00210 (FVF62_00210) - 32819..33511 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  FVF62_RS00215 (FVF62_00215) pilT 33638..34675 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  FVF62_RS00220 (FVF62_00220) pilU 34703..35821 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FVF62_RS00225 (FVF62_00225) fur 35891..36328 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  FVF62_RS00230 (FVF62_00230) - 36440..36838 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  FVF62_RS00235 (FVF62_00235) - 36893..37216 (-) 324 WP_000442587.1 RnfH family protein -
  FVF62_RS00240 (FVF62_00240) - 37216..38283 (-) 1068 WP_002019572.1 hypothetical protein -
  FVF62_RS00245 (FVF62_00250) - 38436..38891 (-) 456 WP_000782976.1 bacteriohemerythrin -
  FVF62_RS00250 (FVF62_00255) - 39144..39992 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  FVF62_RS00255 (FVF62_00260) - 40101..40883 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  FVF62_RS00260 (FVF62_00265) argB 41004..41912 (-) 909 WP_001135419.1 acetylglutamate kinase -
  FVF62_RS00265 (FVF62_00270) - 41927..43345 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  FVF62_RS00270 (FVF62_00275) dut 43545..43997 (-) 453 WP_000868152.1 dUTP diphosphatase -
  FVF62_RS00275 (FVF62_00280) rep 44022..46061 (-) 2040 WP_000093035.1 DNA helicase Rep -
  FVF62_RS00280 (FVF62_00285) - 46255..46908 (-) 654 WP_001202415.1 OmpA family protein -
  FVF62_RS00285 (FVF62_00290) - 47153..48079 (+) 927 WP_000100965.1 acyltransferase -
  FVF62_RS00290 (FVF62_00295) - 48096..49141 (+) 1046 Protein_57 preprotein translocase subunit SecA -
  FVF62_RS00295 (FVF62_00300) minC 49263..49985 (+) 723 WP_000763677.1 septum site-determining protein MinC -
  FVF62_RS00300 (FVF62_00305) minD 50056..50868 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  FVF62_RS00305 (FVF62_00310) minE 50871..51143 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  FVF62_RS00310 (FVF62_00315) - 51227..51514 (+) 288 WP_001218560.1 PA4642 family protein -
  FVF62_RS19415 - 52115..52630 (+) 516 WP_038345456.1 hypothetical protein -
  FVF62_RS00320 (FVF62_00325) rhtC 52686..53306 (-) 621 WP_000959259.1 threonine export protein RhtC -
  FVF62_RS00325 (FVF62_00330) - 53456..56488 (-) 3033 WP_000127823.1 DNA translocase FtsK -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=379481 FVF62_RS00215 WP_000355489.1 33638..34675(+) (pilT) [Acinetobacter baumannii strain ABCR01]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=379481 FVF62_RS00215 WP_000355489.1 33638..34675(+) (pilT) [Acinetobacter baumannii strain ABCR01]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCCGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CAGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTACACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCCATTCAAACTGGTGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGCCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment