Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FVF62_RS00220 Genome accession   NZ_CP042931
Coordinates   34703..35821 (+) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain ABCR01     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 14954..56488 34703..35821 within 0


Gene organization within MGE regions


Location: 14954..56488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVF62_RS00140 (FVF62_00140) - 14954..16111 (-) 1158 WP_000947475.1 tyrosine-type recombinase/integrase -
  FVF62_RS00150 (FVF62_00150) ggt 16809..18794 (-) 1986 WP_001042923.1 gamma-glutamyltransferase -
  FVF62_RS00155 (FVF62_00155) - 19097..20620 (-) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  FVF62_RS00160 (FVF62_00160) - 20627..21778 (-) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  FVF62_RS00165 (FVF62_00165) - 22048..22545 (-) 498 WP_002019577.1 thioesterase family protein -
  FVF62_RS00170 (FVF62_00170) hemB 22688..23701 (+) 1014 WP_034118559.1 porphobilinogen synthase -
  FVF62_RS00175 (FVF62_00175) - 23727..24842 (+) 1116 WP_000546701.1 NAD(P)/FAD-dependent oxidoreductase -
  FVF62_RS00180 (FVF62_00180) - 24834..25739 (-) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  FVF62_RS00185 (FVF62_00185) - 25829..26281 (-) 453 WP_000164153.1 ABZJ_00895 family protein -
  FVF62_RS00190 (FVF62_00190) - 26661..27038 (+) 378 WP_001059438.1 VOC family protein -
  FVF62_RS00195 (FVF62_00195) - 27106..27579 (-) 474 WP_000974358.1 OsmC family protein -
  FVF62_RS00200 (FVF62_00200) - 27743..29011 (+) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  FVF62_RS00205 (FVF62_00205) - 29021..32617 (+) 3597 WP_000698785.1 AAA family ATPase -
  FVF62_RS00210 (FVF62_00210) - 32819..33511 (-) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  FVF62_RS00215 (FVF62_00215) pilT 33638..34675 (+) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  FVF62_RS00220 (FVF62_00220) pilU 34703..35821 (+) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FVF62_RS00225 (FVF62_00225) fur 35891..36328 (-) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  FVF62_RS00230 (FVF62_00230) - 36440..36838 (+) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  FVF62_RS00235 (FVF62_00235) - 36893..37216 (-) 324 WP_000442587.1 RnfH family protein -
  FVF62_RS00240 (FVF62_00240) - 37216..38283 (-) 1068 WP_002019572.1 hypothetical protein -
  FVF62_RS00245 (FVF62_00250) - 38436..38891 (-) 456 WP_000782976.1 bacteriohemerythrin -
  FVF62_RS00250 (FVF62_00255) - 39144..39992 (-) 849 WP_000336548.1 class II glutamine amidotransferase -
  FVF62_RS00255 (FVF62_00260) - 40101..40883 (-) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  FVF62_RS00260 (FVF62_00265) argB 41004..41912 (-) 909 WP_001135419.1 acetylglutamate kinase -
  FVF62_RS00265 (FVF62_00270) - 41927..43345 (-) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  FVF62_RS00270 (FVF62_00275) dut 43545..43997 (-) 453 WP_000868152.1 dUTP diphosphatase -
  FVF62_RS00275 (FVF62_00280) rep 44022..46061 (-) 2040 WP_000093035.1 DNA helicase Rep -
  FVF62_RS00280 (FVF62_00285) - 46255..46908 (-) 654 WP_001202415.1 OmpA family protein -
  FVF62_RS00285 (FVF62_00290) - 47153..48079 (+) 927 WP_000100965.1 acyltransferase -
  FVF62_RS00290 (FVF62_00295) - 48096..49141 (+) 1046 Protein_57 preprotein translocase subunit SecA -
  FVF62_RS00295 (FVF62_00300) minC 49263..49985 (+) 723 WP_000763677.1 septum site-determining protein MinC -
  FVF62_RS00300 (FVF62_00305) minD 50056..50868 (+) 813 WP_001074362.1 septum site-determining protein MinD -
  FVF62_RS00305 (FVF62_00310) minE 50871..51143 (+) 273 WP_000896934.1 cell division topological specificity factor MinE -
  FVF62_RS00310 (FVF62_00315) - 51227..51514 (+) 288 WP_001218560.1 PA4642 family protein -
  FVF62_RS19415 - 52115..52630 (+) 516 WP_038345456.1 hypothetical protein -
  FVF62_RS00320 (FVF62_00325) rhtC 52686..53306 (-) 621 WP_000959259.1 threonine export protein RhtC -
  FVF62_RS00325 (FVF62_00330) - 53456..56488 (-) 3033 WP_000127823.1 DNA translocase FtsK -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=379482 FVF62_RS00220 WP_000347039.1 34703..35821(+) (pilU) [Acinetobacter baumannii strain ABCR01]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=379482 FVF62_RS00220 WP_000347039.1 34703..35821(+) (pilU) [Acinetobacter baumannii strain ABCR01]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395

Multiple sequence alignment