Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FGL86_RS05500 Genome accession   NZ_CP042382
Coordinates   1149044..1150078 (+) Length   344 a.a.
NCBI ID   WP_147183649.1    Uniprot ID   A0A5B8SRM8
Organism   Pistricoccus aurantiacus strain CBA4606     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1144044..1155078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL86_RS05470 (FGL86_05470) - 1144464..1144943 (-) 480 WP_147183643.1 DUF4426 domain-containing protein -
  FGL86_RS05475 (FGL86_05475) metW 1144965..1145558 (-) 594 WP_147183644.1 methionine biosynthesis protein MetW -
  FGL86_RS05480 (FGL86_05480) - 1145558..1146706 (-) 1149 WP_147183645.1 homoserine O-acetyltransferase -
  FGL86_RS05485 (FGL86_05485) - 1146706..1147296 (-) 591 WP_147183646.1 YggT family protein -
  FGL86_RS05490 (FGL86_05490) proC 1147389..1148216 (-) 828 WP_147183647.1 pyrroline-5-carboxylate reductase -
  FGL86_RS05495 (FGL86_05495) - 1148266..1148970 (-) 705 WP_147183648.1 YggS family pyridoxal phosphate-dependent enzyme -
  FGL86_RS05500 (FGL86_05500) pilT 1149044..1150078 (+) 1035 WP_147183649.1 type IV pilus twitching motility protein PilT Machinery gene
  FGL86_RS05505 (FGL86_05505) pilU 1150126..1151292 (+) 1167 WP_281288532.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FGL86_RS05510 (FGL86_05510) - 1151289..1152422 (-) 1134 WP_147183650.1 anhydro-N-acetylmuramic acid kinase -
  FGL86_RS05515 (FGL86_05515) tyrS 1152544..1153740 (+) 1197 WP_147183651.1 tyrosine--tRNA ligase -
  FGL86_RS05520 (FGL86_05520) - 1153852..1154307 (+) 456 WP_147183652.1 copper resistance protein NlpE N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37608.10 Da        Isoelectric Point: 6.3490

>NTDB_id=377524 FGL86_RS05500 WP_147183649.1 1149044..1150078(+) (pilT) [Pistricoccus aurantiacus strain CBA4606]
MDITELLVFSAKENASDLHLSAGMPPMIRVDGDIRRLSAPAMDDSEVRKLIYDIMGDRQRKDYEEFFETDFSFEVPGISR
FRVNAFNHGRGAGAVFRTIPSEVLSMERLGLGSAFRRLASLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRHDHILTIED
PVEFVHASKRCLINQREVHRDTLGFAPALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKAMVRSMLSESLQGVVAQTLLKKIGGGRVAAHEILIATPAIRNLIREDKVAQIYSAIQTGGGQGMQTLDSAL
MRLINEGLVDRDEAQAKAKGALAA

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=377524 FGL86_RS05500 WP_147183649.1 1149044..1150078(+) (pilT) [Pistricoccus aurantiacus strain CBA4606]
ATGGATATTACCGAACTGCTGGTGTTTTCGGCAAAAGAGAATGCCTCCGACCTGCACCTGTCCGCCGGGATGCCGCCGAT
GATCCGCGTCGATGGCGATATTCGGCGCCTCAGCGCGCCGGCAATGGACGACAGCGAAGTCCGCAAGCTTATCTACGACA
TCATGGGGGATCGGCAGCGCAAGGACTATGAAGAATTCTTCGAAACCGATTTTTCTTTCGAGGTCCCCGGGATTTCGCGC
TTTCGTGTCAACGCCTTCAATCACGGGCGCGGCGCCGGCGCGGTTTTTCGTACCATCCCCTCGGAAGTACTCAGCATGGA
GCGGCTGGGGCTCGGCTCCGCGTTCCGACGCCTGGCGTCATTGCCTCGCGGGCTGGTGCTAGTCACCGGCCCTACCGGCT
CCGGCAAGAGCACTACCCTGGCGGCAATGATCGACTATATCAACGATCACCGCCACGACCACATACTGACCATCGAAGAT
CCGGTGGAATTCGTTCACGCCAGCAAGCGTTGCCTGATCAATCAGCGGGAAGTGCACCGGGATACCCTGGGCTTTGCCCC
CGCCCTGCGCAGCGCCCTGCGGGAAGACCCGGACGTGATCCTCGTCGGCGAACTGCGTGACCTGGAAACCATTCGTCTGG
CGTTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCATACCACTTCTGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCGGGAGATGAAAAGGCCATGGTGCGCTCCATGCTCTCGGAGTCCCTGCAAGGCGTCGTCGCCCAGAC
CCTGCTCAAGAAGATCGGCGGCGGGCGAGTGGCGGCCCACGAGATTCTGATAGCAACCCCGGCGATTCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATCTATTCCGCCATTCAGACTGGCGGCGGCCAGGGCATGCAGACCCTGGACTCCGCCTTG
ATGCGCCTCATCAACGAGGGCTTGGTGGATCGAGACGAGGCGCAGGCCAAGGCAAAAGGAGCGCTCGCGGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8SRM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.171

98.547

0.77
  pilT Pseudomonas aeruginosa PAK

77.581

98.547

0.765
  pilT Acinetobacter baylyi ADP1

76.106

98.547

0.75
  pilT Acinetobacter nosocomialis M2

74.631

98.547

0.735
  pilT Acinetobacter baumannii D1279779

74.631

98.547

0.735
  pilT Acinetobacter baumannii strain A118

74.631

98.547

0.735
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.023

100

0.68
  pilT Vibrio cholerae strain A1552

68.023

100

0.68
  pilT Neisseria meningitidis 8013

65.789

99.419

0.654
  pilT Neisseria gonorrhoeae MS11

65.497

99.419

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.917

98.547

0.512
  pilU Vibrio cholerae strain A1552

40.351

99.419

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

40.979

95.058

0.39

Multiple sequence alignment