Detailed information NTG0377525

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FGL86_RS05505
• Genome accession: NZ_CP042382
• Coordinates: 1150126..1151292 (+)
• Length: 388 a.a.
• NCBI ID: WP_281288532.1
• Uniprot ID: -
• Organism: Pistricoccus aurantiacus strain CBA4606
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1145126..1156292

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FGL86_RS05480 (FGL86_05480)	-	1145558..1146706 (-)	1149	WP_147183645.1	homoserine O-acetyltransferase	-
FGL86_RS05485 (FGL86_05485)	-	1146706..1147296 (-)	591	WP_147183646.1	YggT family protein	-
FGL86_RS05490 (FGL86_05490)	proC	1147389..1148216 (-)	828	WP_147183647.1	pyrroline-5-carboxylate reductase	-
FGL86_RS05495 (FGL86_05495)	-	1148266..1148970 (-)	705	WP_147183648.1	YggS family pyridoxal phosphate-dependent enzyme	-
FGL86_RS05500 (FGL86_05500)	pilT	1149044..1150078 (+)	1035	WP_147183649.1	type IV pilus twitching motility protein PilT	Machinery gene
FGL86_RS05505 (FGL86_05505)	pilU	1150126..1151292 (+)	1167	WP_281288532.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FGL86_RS05510 (FGL86_05510)	-	1151289..1152422 (-)	1134	WP_147183650.1	anhydro-N-acetylmuramic acid kinase	-
FGL86_RS05515 (FGL86_05515)	tyrS	1152544..1153740 (+)	1197	WP_147183651.1	tyrosine--tRNA ligase	-
FGL86_RS05520 (FGL86_05520)	-	1153852..1154307 (+)	456	WP_147183652.1	copper resistance protein NlpE N-terminal domain-containing protein	-
FGL86_RS05525 (FGL86_05525)	radA	1154410..1155783 (+)	1374	WP_147183653.1	DNA repair protein RadA	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 42964.22 Da
• Isoelectric Point: 6.9784

>NTDB_id=377525 FGL86_RS05505 WP_281288532.1 1150126..1151292(+) (pilU) [Pistricoccus aurantiacus strain CBA4606]
MTDHADAESARKWLHQLLSTVIKREASDLLISINAPPTLKMAGRMTPLGEAPLSQAQVKALVNVALPDSQRERLQREQEA
NFTLSVPRLGRFRVSAFQQRGQPAMVLRRISFDIPTLESLSLPASLGDLVTIKRGLVFVVGGTGSGKSSTLAALIQRRNT
TIGGHILCIEDPIEYIHAHQNAIINQREVGVDTESFEIALKNTLRQAPDVIMIGEVRSQETMQHALSFAETGHLCLATLH
ANNANQALDRIINFFPVERHPQVWLDLSLNLRAIVAQQLLPATNGGRQVAVEVLRHSPLIGDLIRKGKVPEIKELIGRSN
DLGMQTFDQSLYRLFREGLISEDVALVHADSANELRLMIQFGHETGEAANQVKENLRHLRLQEDGEGF

Nucleotide

• Length: 1167 bp

>NTDB_id=377525 FGL86_RS05505 WP_281288532.1 1150126..1151292(+) (pilU) [Pistricoccus aurantiacus strain CBA4606]
ATCACCGATCATGCCGACGCGGAAAGTGCCCGGAAATGGCTTCATCAACTGCTGAGCACCGTCATCAAGCGCGAGGCCTC
GGATCTCTTGATATCGATCAATGCGCCGCCGACGTTGAAAATGGCAGGACGCATGACGCCTTTGGGCGAGGCACCCTTGA
GCCAGGCGCAGGTCAAGGCGTTGGTCAATGTTGCGCTTCCGGACAGCCAGCGGGAGCGCCTGCAGCGAGAGCAGGAAGCC
AACTTCACGCTGAGCGTGCCGCGGCTGGGTCGTTTTCGCGTCAGCGCCTTTCAGCAGCGCGGCCAGCCGGCGATGGTGCT
GCGACGGATTTCCTTCGATATACCCACTTTGGAATCCCTGTCGCTGCCCGCCTCCCTGGGAGATCTGGTGACCATCAAGC
GCGGGCTGGTGTTCGTGGTGGGCGGCACCGGCAGCGGCAAGTCCTCGACGCTGGCGGCGCTGATCCAGCGACGCAATACC
ACCATCGGCGGTCATATTTTGTGTATCGAAGATCCCATCGAATATATACACGCCCATCAGAACGCGATCATCAATCAGCG
CGAGGTGGGGGTGGATACGGAATCCTTCGAGATCGCCCTCAAGAACACCCTGCGCCAGGCGCCGGATGTCATCATGATCG
GCGAGGTGCGCTCCCAGGAAACCATGCAGCATGCGCTTTCCTTCGCGGAAACCGGCCATCTGTGCCTGGCCACGCTACAT
GCCAACAACGCCAACCAGGCGCTGGATCGCATCATCAACTTCTTCCCCGTGGAGCGTCATCCCCAGGTCTGGCTCGATCT
TTCCCTGAACCTGCGAGCCATTGTCGCCCAGCAGCTGCTGCCGGCGACTAACGGCGGGCGTCAGGTGGCGGTGGAGGTCC
TGCGTCACTCGCCCTTGATCGGCGACCTGATCCGCAAGGGCAAGGTGCCGGAGATCAAGGAGTTGATCGGGCGCTCCAAC
GATCTGGGCATGCAAACCTTCGATCAATCGCTGTATCGGCTTTTCAGGGAAGGCCTCATCAGCGAGGACGTGGCCCTGGT
GCATGCGGATTCCGCCAACGAGCTGCGCCTGATGATTCAATTCGGTCACGAAACCGGCGAGGCGGCGAACCAGGTCAAGG
AAAACCTGCGCCATTTGAGGCTGCAGGAAGACGGCGAGGGCTTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (72-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.915	96.907	0.552
pilU	Vibrio cholerae strain A1552	54.885	89.691	0.492
pilU	Acinetobacter baylyi ADP1	52.528	91.753	0.482
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.691	88.402	0.369