Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RPSI07_RS11730 Genome accession   NC_014311
Coordinates   836447..837490 (+) Length   347 a.a.
NCBI ID   WP_013211545.1    Uniprot ID   A0AAD0S5Z5
Organism   Ralstonia solanacearum PSI07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 831447..842490
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPSI07_RS11700 (RPSI07_0824) - 832603..832845 (+) 243 WP_013211540.1 hypothetical protein -
  RPSI07_RS11705 (RPSI07_0825) - 833007..833507 (+) 501 WP_003271865.1 Dps family protein -
  RPSI07_RS11710 (RPSI07_0826) ubiA 833581..834456 (+) 876 WP_013211541.1 4-hydroxybenzoate octaprenyltransferase -
  RPSI07_RS11715 (RPSI07_0827) - 834453..834731 (-) 279 WP_013211542.1 hypothetical protein -
  RPSI07_RS11720 (RPSI07_0828) proC 834744..835568 (-) 825 WP_013211543.1 pyrroline-5-carboxylate reductase -
  RPSI07_RS11725 (RPSI07_0829) - 835603..836325 (-) 723 WP_013211544.1 YggS family pyridoxal phosphate-dependent enzyme -
  RPSI07_RS11730 (RPSI07_0830) pilT 836447..837490 (+) 1044 WP_013211545.1 type IV pilus twitching motility protein PilT Machinery gene
  RPSI07_RS11735 (RPSI07_0831) pilU 837543..838679 (+) 1137 WP_013211546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RPSI07_RS26575 - 838874..839314 (+) 441 Protein_775 type IV pilin protein -
  RPSI07_RS11745 (RPSI07_0834) - 839318..839806 (+) 489 WP_048817334.1 GspH/FimT family pseudopilin -
  RPSI07_RS11750 (RPSI07_0835) pilV 839803..840393 (+) 591 WP_013211549.1 type IV pilus modification protein PilV -
  RPSI07_RS11755 (RPSI07_0836) - 840390..841412 (+) 1023 WP_013211550.1 PilW family protein -
  RPSI07_RS11760 (RPSI07_0838) - 841416..841942 (+) 527 Protein_779 pilus assembly PilX family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38251.91 Da        Isoelectric Point: 6.8706

>NTDB_id=37736 RPSI07_RS11730 WP_013211545.1 836447..837490(+) (pilT) [Ralstonia solanacearum PSI07]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLTLEDLKTPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPSEEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=37736 RPSI07_RS11730 WP_013211545.1 836447..837490(+) (pilT) [Ralstonia solanacearum PSI07]
ATGGACATCGCGCAGCTTCTGGCCTTCGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCGCCGAT
GATCCGGATCCATGGCGACATGCGCCGTATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCCGACTTCTCGTTCGAGATTCCCGGGCTGTCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGCGGCGCCTCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGACGCCGGCCGTCTTCGCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAATCACCGCAATGAAAACGATCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAATTGGGGCCGCATACGCACTCGTTTGCCAA
TGCGCTGAAATCGGCGCTGCGTGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACGGCGGCCGAAACCGGCCACTTGGTGTTTGCCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGAAGAGAAGGACATGGTCCGCACCATGCTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCTGCGCACGAGATCATGATCTGCACGCCGGCCATCCGCCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGGCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAATCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.884

99.424

0.715
  pilT Acinetobacter baumannii D1279779

71.884

99.424

0.715
  pilT Acinetobacter baumannii strain A118

71.884

99.424

0.715
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

67.63

99.712

0.674
  pilT Neisseria meningitidis 8013

67.63

99.712

0.674
  pilT Legionella pneumophila strain Lp02

67.246

99.424

0.669
  pilT Legionella pneumophila strain ERS1305867

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.875

97.118

0.64
  pilT Vibrio cholerae strain A1552

65.875

97.118

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.867

99.712

0.507
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409

Multiple sequence alignment