Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RPSI07_RS11735 Genome accession   NC_014311
Coordinates   837543..838679 (+) Length   378 a.a.
NCBI ID   WP_013211546.1    Uniprot ID   A0AAD0S6R2
Organism   Ralstonia solanacearum PSI07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 832543..843679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPSI07_RS11700 (RPSI07_0824) - 832603..832845 (+) 243 WP_013211540.1 hypothetical protein -
  RPSI07_RS11705 (RPSI07_0825) - 833007..833507 (+) 501 WP_003271865.1 Dps family protein -
  RPSI07_RS11710 (RPSI07_0826) ubiA 833581..834456 (+) 876 WP_013211541.1 4-hydroxybenzoate octaprenyltransferase -
  RPSI07_RS11715 (RPSI07_0827) - 834453..834731 (-) 279 WP_013211542.1 hypothetical protein -
  RPSI07_RS11720 (RPSI07_0828) proC 834744..835568 (-) 825 WP_013211543.1 pyrroline-5-carboxylate reductase -
  RPSI07_RS11725 (RPSI07_0829) - 835603..836325 (-) 723 WP_013211544.1 YggS family pyridoxal phosphate-dependent enzyme -
  RPSI07_RS11730 (RPSI07_0830) pilT 836447..837490 (+) 1044 WP_013211545.1 type IV pilus twitching motility protein PilT Machinery gene
  RPSI07_RS11735 (RPSI07_0831) pilU 837543..838679 (+) 1137 WP_013211546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RPSI07_RS26575 - 838874..839314 (+) 441 Protein_775 type IV pilin protein -
  RPSI07_RS11745 (RPSI07_0834) - 839318..839806 (+) 489 WP_048817334.1 GspH/FimT family pseudopilin -
  RPSI07_RS11750 (RPSI07_0835) pilV 839803..840393 (+) 591 WP_013211549.1 type IV pilus modification protein PilV -
  RPSI07_RS11755 (RPSI07_0836) - 840390..841412 (+) 1023 WP_013211550.1 PilW family protein -
  RPSI07_RS11760 (RPSI07_0838) - 841416..841942 (+) 527 Protein_779 pilus assembly PilX family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42053.53 Da        Isoelectric Point: 7.0670

>NTDB_id=37737 RPSI07_RS11735 WP_013211546.1 837543..838679(+) (pilU) [Ralstonia solanacearum PSI07]
MLDREAATKYIHELLQLMVNSRGSDLFITSEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREFEGSFECNFA
IVSGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSYG
HIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANSS
NQAIDRIINFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGRKGRAPAIEILLGTPLVADLIFKGEVHELKEVMKKSREQG
MVSFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHSKRGGPVDLTAGTEHLNVM

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=37737 RPSI07_RS11735 WP_013211546.1 837543..838679(+) (pilU) [Ralstonia solanacearum PSI07]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGATTTGTT
CATCACCTCGGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAGTTGCGCGAGTTCGAAGGCTCCTTCGAGTGCAACTTTGCC
ATCGTGTCCGGCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCATCAA
CACCAAGATCCCGACGCTCGGCGAACTGGATCTGCCGCCCATCCTCAACGAGGTCGCGATGAGCAAGCGCGGGCTCGTGG
TCGTGGTGGGGGCGACGGGCTCCGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTACGGC
CACATCATCACCATTGAAGACCCCGTGGAATACGTGCACGCACACCAGAACTGCGTCGTGACGCAGCGCGAGGTGGGCGT
GGATACGGAGTCGTGGCACGTGGCGCTGAAGAACACGTTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCG
ATCGCGACACGATGGAGTACGCCATCCAGTACGCCGAAACCGGTCACTTGTGCCTGGCCACGCTGCACGCCAACAGCTCG
AACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCCAGCAGCTGCTGATCGACCTGTCCTTGAACCT
GCGCGCCATGATCGCGCAGCGGCTGCTGCCGCGCAAAGGCAGGAAGGGCCGCGCGCCGGCGATCGAAATCCTGCTGGGCA
CGCCGTTGGTGGCCGATCTGATCTTCAAGGGCGAGGTGCATGAGCTCAAGGAGGTCATGAAGAAGTCCCGCGAGCAGGGG
ATGGTCTCGTTCGACCAGGCGTTGTTCGAACTCTATGAGGCGGACAAGATCACCTATGAGGATGCGCTGCGCAACGCAGA
CTCGCTGAACGATCTGCGTCTGCAGATCAAGCTGCACAGCAAGCGCGGCGGGCCGGTGGACCTGACGGCAGGCACCGAGC
ATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.606

93.386

0.585
  pilU Vibrio cholerae strain A1552

56.091

93.386

0.524
  pilU Acinetobacter baylyi ADP1

54.596

94.974

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas aeruginosa PAK

42.73

89.153

0.381
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Vibrio cholerae strain A1552

44.795

83.862

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.795

83.862

0.376
  pilT Acinetobacter baylyi ADP1

44.099

85.185

0.376
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter baumannii D1279779

43.26

84.392

0.365
  pilT Acinetobacter baumannii strain A118

43.26

84.392

0.365
  pilT Acinetobacter nosocomialis M2

43.26

84.392

0.365

Multiple sequence alignment