Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOZ74_RS06225 Genome accession   NZ_CP042344
Coordinates   1276702..1277745 (-) Length   347 a.a.
NCBI ID   WP_146912253.1    Uniprot ID   A0A5B8RV72
Organism   Comamonas flocculans strain NLF-7-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1271702..1282745
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOZ74_RS06200 (FOZ74_06200) - 1271796..1272908 (+) 1113 WP_255437842.1 phospholipase A -
  FOZ74_RS06205 (FOZ74_06205) - 1273103..1273813 (+) 711 WP_146912249.1 BON domain-containing protein -
  FOZ74_RS06210 (FOZ74_06210) - 1273860..1274795 (-) 936 WP_146912250.1 NAD(P)-dependent oxidoreductase -
  FOZ74_RS06215 (FOZ74_06215) pilU 1274814..1275950 (-) 1137 WP_146912251.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOZ74_RS06220 (FOZ74_06220) - 1275993..1276622 (-) 630 WP_146912252.1 cyclic nucleotide-binding domain-containing protein -
  FOZ74_RS06225 (FOZ74_06225) pilT 1276702..1277745 (-) 1044 WP_146912253.1 type IV pilus twitching motility protein PilT Machinery gene
  FOZ74_RS06230 (FOZ74_06230) - 1277793..1278554 (+) 762 WP_146912254.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOZ74_RS06235 (FOZ74_06235) - 1278620..1279756 (+) 1137 WP_146912255.1 aminotransferase class V-fold PLP-dependent enzyme -
  FOZ74_RS06240 (FOZ74_06240) - 1279776..1281197 (-) 1422 WP_146912256.1 GTPase/DUF3482 domain-containing protein -
  FOZ74_RS06245 (FOZ74_06245) - 1281194..1282630 (-) 1437 WP_146912257.1 DUF2868 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.6647

>NTDB_id=377183 FOZ74_RS06225 WP_146912253.1 1276702..1277745(-) (pilT) [Comamonas flocculans strain NLF-7-7]
MDITQLLAFSVKNNASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKIYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNETEYGHILTIED
PIEFVHESRKCLVNQREVGPMTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGNSQGMQTLDQN
LTELVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=377183 FOZ74_RS06225 WP_146912253.1 1276702..1277745(-) (pilT) [Comamonas flocculans strain NLF-7-7]
GTGGACATCACCCAGCTACTAGCCTTCAGCGTGAAGAACAACGCCTCGGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGCGTGCATGGCGACGTACGGCGCATCAACGTCGATGCGCTGGACCACAAGACGGTACATGCCATGGTGTACGACA
TCATGAGCGACGCGCAGCGCAAGATCTACGAAGAATTCCTCGAGGTGGACTTTTCCTTCGAGATCGACGGCCTGGCGCGT
TTTCGCGTCAATGCCTTCAACCAGAACCGCGGTGCGGCAGCGGTGTTCCGCACGATTCCGAGCAAGATCCTGACGCTCGA
GCAGCTCAATGCACCCAAGATCTTCGCCGACCTGGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGGCCCACGGGCT
CGGGCAAGTCCACGACGCTGGCGGCCATGGTCAACCACCTGAACGAAACCGAGTACGGCCACATCCTGACCATCGAGGAC
CCGATCGAGTTCGTGCACGAATCGCGCAAGTGCCTGGTGAACCAGCGCGAAGTCGGGCCGATGACGCTGTCGTTCTCGGC
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAGATGCGCGACCTCGAAACCATCCGCCTGG
CGATGACGGCCGCCGAAACCGGGCACCTGGTGTTCGGCACGCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGGGTC
ATCGACGTCTTTCCGGCCGAGGAAAAGGACATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGCCGTGTGGCCGCGCACGAGATCATGCTGGGCACCAGCGCGATCCGCAACCTGA
TCCGCGAGGCTAAGGTGGCGCAGATGTATTCCACCATCCAGACCGGCAACAGCCAGGGCATGCAGACGCTGGACCAGAAC
CTCACCGAGCTGGTGCGGCGCAACATCATCAGCCCGGCCGAAGCGCGCAGCAAGGCCAAGATACCGGAGAATTTCCCCGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8RV72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Pseudomonas stutzeri DSM 10701

77.391

99.424

0.769
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Neisseria meningitidis 8013

66.957

99.424

0.666
  pilT Neisseria gonorrhoeae MS11

66.667

99.424

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Acinetobacter baylyi ADP1

39.595

99.712

0.395

Multiple sequence alignment