Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FOZ74_RS06215 Genome accession   NZ_CP042344
Coordinates   1274814..1275950 (-) Length   378 a.a.
NCBI ID   WP_146912251.1    Uniprot ID   A0A5B8RSY7
Organism   Comamonas flocculans strain NLF-7-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1269814..1280950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOZ74_RS06200 (FOZ74_06200) - 1271796..1272908 (+) 1113 WP_255437842.1 phospholipase A -
  FOZ74_RS06205 (FOZ74_06205) - 1273103..1273813 (+) 711 WP_146912249.1 BON domain-containing protein -
  FOZ74_RS06210 (FOZ74_06210) - 1273860..1274795 (-) 936 WP_146912250.1 NAD(P)-dependent oxidoreductase -
  FOZ74_RS06215 (FOZ74_06215) pilU 1274814..1275950 (-) 1137 WP_146912251.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FOZ74_RS06220 (FOZ74_06220) - 1275993..1276622 (-) 630 WP_146912252.1 cyclic nucleotide-binding domain-containing protein -
  FOZ74_RS06225 (FOZ74_06225) pilT 1276702..1277745 (-) 1044 WP_146912253.1 type IV pilus twitching motility protein PilT Machinery gene
  FOZ74_RS06230 (FOZ74_06230) - 1277793..1278554 (+) 762 WP_146912254.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOZ74_RS06235 (FOZ74_06235) - 1278620..1279756 (+) 1137 WP_146912255.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41757.20 Da        Isoelectric Point: 6.6113

>NTDB_id=377182 FOZ74_RS06215 WP_146912251.1 1274814..1275950(-) (pilU) [Comamonas flocculans strain NLF-7-7]
MERDQASKFINDLLKLMVSRGGSDLFITADFPPAIKVDGKMTKVSPQPLTAMHTLTLARSIMSDKQVADFERTKECNFAI
SPVGIGRFRVNAFIQQGNVGMVLRTIPLSLPSIDSLGLPQVLKEVAMTKRGLCIMVGATGSGKSTSLAAMVDWRNENSYG
HIVTVEDPIEFVHAHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVVFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRALVSQRLVPKQDGKGRIAAVEIMLNSPLIADFIFKGEVAEIKDVMKKSRNLG
MQTFDQALFDLFEANLISYEDALRNADSLNDLRLQIKLSSQRAKSGDLAAGTEQFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=377182 FOZ74_RS06215 WP_146912251.1 1274814..1275950(-) (pilU) [Comamonas flocculans strain NLF-7-7]
ATGGAACGCGATCAGGCAAGCAAATTCATCAACGACCTGCTCAAGCTGATGGTCAGCCGCGGTGGCAGCGACCTGTTCAT
CACCGCGGATTTTCCGCCGGCCATCAAGGTGGACGGCAAGATGACCAAGGTCTCGCCCCAGCCGCTCACGGCCATGCACA
CGCTGACCCTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGATTTCGAGCGCACCAAGGAATGCAACTTCGCCATC
TCGCCCGTGGGCATAGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCAACGTCGGCATGGTGCTGCGCACCATCCC
GCTGAGCCTGCCGTCCATCGACAGCCTGGGCTTGCCGCAGGTGCTCAAGGAAGTGGCGATGACCAAGCGCGGCCTGTGCA
TCATGGTCGGCGCCACCGGCTCGGGCAAGTCCACGTCGCTCGCGGCCATGGTGGACTGGCGCAATGAAAACTCCTACGGC
CACATCGTGACGGTGGAAGACCCGATCGAGTTCGTGCATGCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGGCT
CGATACCGACAGCTGGGAGGCGGCGCTCAAGAACACCTTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGAGCATGCGGTGGTCTTTTCCGAGACCGGCCACCTGTGCCTGGCTACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCTGAAGAGCGGCGCGCGCAGTTGCTCATGGACTTGTCGCTGAACCT
GCGCGCACTGGTCTCGCAGCGCCTGGTGCCCAAGCAGGACGGCAAGGGGCGCATCGCGGCGGTGGAGATCATGCTCAATT
CGCCCCTGATTGCCGACTTCATCTTCAAGGGCGAGGTGGCCGAAATCAAGGACGTGATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTTCGAGGCCAACCTCATCAGCTACGAGGATGCGCTGCGCAACGCCGA
TTCGCTCAACGACCTGCGCCTGCAGATCAAGCTGTCGAGCCAGCGCGCCAAGTCGGGCGACCTGGCGGCGGGCACGGAAC
AATTCGCCATCGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8RSY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.073

94.709

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Legionella pneumophila strain Lp02

44.745

88.095

0.394
  pilT Legionella pneumophila strain ERS1305867

44.745

88.095

0.394
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment