Detailed information NTG0376231

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: G9P50_RS03610
• Genome accession: NZ_CP050040
• Coordinates: 756311..757291 (+)
• Length: 326 a.a.
• NCBI ID: WP_001295381.1
• Uniprot ID: -
• Organism: Escherichia coli strain 63-2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 751311..762291

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G9P50_RS03580 (G9P50_03580)	yggM	751826..752833 (+)	1008	WP_001753044.1	DUF1202 family protein	-
G9P50_RS03585 (G9P50_03585)	hemW	752988..754124 (-)	1137	WP_000239928.1	radical SAM family heme chaperone HemW	-
G9P50_RS03590 (G9P50_03590)	rdgB	754117..754710 (-)	594	WP_001174735.1	XTP/dITP diphosphatase	-
G9P50_RS03595 (G9P50_03595)	yggU	754718..755008 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
G9P50_RS03600 (G9P50_03600)	yggT	755005..755571 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
G9P50_RS03605 (G9P50_03605)	yggS	755589..756293 (-)	705	WP_001424369.1	pyridoxal phosphate homeostasis protein	-
G9P50_RS03610 (G9P50_03610)	pilT	756311..757291 (+)	981	WP_001295381.1	type IV pilus twitching motility protein PilT	Machinery gene
G9P50_RS22360	-	757340..757468 (+)	129	WP_001278270.1	hypothetical protein	-
G9P50_RS03615 (G9P50_03615)	ruvX	757475..757891 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
G9P50_RS03620 (G9P50_03620)	yqgE	757891..758454 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
G9P50_RS03625 (G9P50_03625)	gshB	758563..759513 (-)	951	WP_000593273.1	glutathione synthase	-
G9P50_RS03630 (G9P50_03630)	rsmE	759526..760257 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
G9P50_RS03635 (G9P50_03635)	endA	760337..761044 (-)	708	WP_000286500.1	deoxyribonuclease I	-
G9P50_RS03640 (G9P50_03640)	yggI	761139..761636 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35926.14 Da
• Isoelectric Point: 5.7654

>NTDB_id=376231 G9P50_RS03610 WP_001295381.1 756311..757291(+) (pilT) [Escherichia coli strain 63-2]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=376231 G9P50_RS03610 WP_001295381.1 756311..757291(+) (pilT) [Escherichia coli strain 63-2]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGTTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Neisseria meningitidis 8013	49.085	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.78	100	0.491
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.177	100	0.463
pilT	Acinetobacter baylyi ADP1	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.062	98.773	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.273	100	0.377
pilU	Acinetobacter baylyi ADP1	37.048	100	0.377
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362