Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FPZ22_RS04485 Genome accession   NZ_CP042218
Coordinates   970284..971321 (-) Length   345 a.a.
NCBI ID   WP_144890799.1    Uniprot ID   A0A518N2T0
Organism   Luteimonas granuli strain Gr-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 965284..976321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPZ22_RS04460 (FPZ22_04460) - 966287..967234 (-) 948 WP_144890789.1 aspartate carbamoyltransferase catalytic subunit -
  FPZ22_RS04465 (FPZ22_04465) ruvX 967231..967719 (-) 489 WP_144890791.1 Holliday junction resolvase RuvX -
  FPZ22_RS04470 (FPZ22_04470) - 967719..968285 (-) 567 WP_144890793.1 YqgE/AlgH family protein -
  FPZ22_RS04475 (FPZ22_04475) - 968377..969003 (-) 627 WP_144890795.1 superoxide dismutase -
  FPZ22_RS04480 (FPZ22_04480) pilU 969141..970271 (-) 1131 WP_144890797.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FPZ22_RS04485 (FPZ22_04485) pilT 970284..971321 (-) 1038 WP_144890799.1 type IV pilus twitching motility protein PilT Machinery gene
  FPZ22_RS04490 (FPZ22_04490) - 971457..972155 (+) 699 WP_144890801.1 YggS family pyridoxal phosphate-dependent enzyme -
  FPZ22_RS04495 (FPZ22_04495) proC 972239..973078 (+) 840 WP_144890803.1 pyrroline-5-carboxylate reductase -
  FPZ22_RS04500 (FPZ22_04500) - 973095..973490 (-) 396 WP_210412706.1 DoxX family protein -
  FPZ22_RS04505 (FPZ22_04505) - 973565..974386 (-) 822 WP_144890805.1 cobalamin-binding protein -
  FPZ22_RS04510 (FPZ22_04510) pdxH 974383..974979 (-) 597 WP_144890807.1 pyridoxamine 5'-phosphate oxidase -
  FPZ22_RS04515 (FPZ22_04515) - 975070..975942 (+) 873 WP_246120930.1 kinase -
  FPZ22_RS04520 (FPZ22_04520) - 975923..976126 (-) 204 WP_144890811.1 dodecin family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38602.31 Da        Isoelectric Point: 6.7836

>NTDB_id=376055 FPZ22_RS04485 WP_144890799.1 970284..971321(-) (pilT) [Luteimonas granuli strain Gr-4]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINVPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIAGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGTPRIFKELIDQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHILTIED
PIEFVHTAQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQQHGMMTLDQHL
QDLVKRSMVTRQQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=376055 FPZ22_RS04485 WP_144890799.1 970284..971321(-) (pilT) [Luteimonas granuli strain Gr-4]
ATGGATATCGCCGAACTCCTGGCGTTTTCGGTCAAGAACAAAGCCTCGGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTCCGGCGCATCAACGTTCCGGCGCTGGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCGTTCGAGATCGCCGGGCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAATCGCGGCGCGGGAGCGGTGTTCCGCACCATTCCATCCGAGGTGCTGACGCTGGA
GGACCTCGGTACGCCGCGGATCTTCAAGGAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACGGGTT
CGGGCAAGTCGACCACGCTGGCGGCCATGGTCGACCACATCAACAAGAACGAGTACGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACACCGCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCCCTGCGCTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAACTGCGCGACCTGGAGACCATCCGCCTCG
CACTGACCGCCGCCGAGACGGGCCACCTGGTGTTCGGCACCCTGCATACCTCCAGCGCCGCCAAGACCATCGATCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCCCAGGC
CCTGCTGAAGAAGGTCGGCGGTGGCCGCACCGCCGCCTGGGAGATCATGGTCGGCATTCCCGCCATCAGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGCACGGCATGATGACGCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCATGGTGACCCGCCAGCAGGCCCGGGACTACGCCAAGGACAAGCGGCTCTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A518N2T0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.746

97.101

0.687
  pilT Vibrio cholerae strain A1552

70.746

97.101

0.687
  pilT Neisseria meningitidis 8013

64.327

99.13

0.638
  pilT Neisseria gonorrhoeae MS11

64.035

99.13

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394

Multiple sequence alignment