Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FPZ22_RS04480 Genome accession   NZ_CP042218
Coordinates   969141..970271 (-) Length   376 a.a.
NCBI ID   WP_144890797.1    Uniprot ID   A0A518N2U7
Organism   Luteimonas granuli strain Gr-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 964141..975271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPZ22_RS04455 (FPZ22_04455) mgtE 964906..966267 (+) 1362 WP_144890787.1 magnesium transporter -
  FPZ22_RS04460 (FPZ22_04460) - 966287..967234 (-) 948 WP_144890789.1 aspartate carbamoyltransferase catalytic subunit -
  FPZ22_RS04465 (FPZ22_04465) ruvX 967231..967719 (-) 489 WP_144890791.1 Holliday junction resolvase RuvX -
  FPZ22_RS04470 (FPZ22_04470) - 967719..968285 (-) 567 WP_144890793.1 YqgE/AlgH family protein -
  FPZ22_RS04475 (FPZ22_04475) - 968377..969003 (-) 627 WP_144890795.1 superoxide dismutase -
  FPZ22_RS04480 (FPZ22_04480) pilU 969141..970271 (-) 1131 WP_144890797.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FPZ22_RS04485 (FPZ22_04485) pilT 970284..971321 (-) 1038 WP_144890799.1 type IV pilus twitching motility protein PilT Machinery gene
  FPZ22_RS04490 (FPZ22_04490) - 971457..972155 (+) 699 WP_144890801.1 YggS family pyridoxal phosphate-dependent enzyme -
  FPZ22_RS04495 (FPZ22_04495) proC 972239..973078 (+) 840 WP_144890803.1 pyrroline-5-carboxylate reductase -
  FPZ22_RS04500 (FPZ22_04500) - 973095..973490 (-) 396 WP_210412706.1 DoxX family protein -
  FPZ22_RS04505 (FPZ22_04505) - 973565..974386 (-) 822 WP_144890805.1 cobalamin-binding protein -
  FPZ22_RS04510 (FPZ22_04510) pdxH 974383..974979 (-) 597 WP_144890807.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41836.07 Da        Isoelectric Point: 6.7224

>NTDB_id=376054 FPZ22_RS04480 WP_144890797.1 969141..970271(-) (pilU) [Luteimonas granuli strain Gr-4]
MSAIDFTSYLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMTPPQREEFERTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIESRIPTIEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSANEVRLRIKLAQGGDARTLAQGLDGVEVAEAN

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=376054 FPZ22_RS04480 WP_144890797.1 969141..970271(-) (pilU) [Luteimonas granuli strain Gr-4]
ATGAGCGCCATCGATTTCACCTCCTACCTCAAGCTGATGGCCCACCAGAAGGCGTCGGACCTGTTCATCACGGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCGATCACCCAGAACCCGCTGACGCCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTGATGACGCCGCCGCAGCGCGAGGAGTTCGAGCGCACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGC
GTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGTCCAGGATCCC
GACGATCGAAGAGCTGAACCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACGGGCAAATCCACCTCGCTGGCGTCGATGATCGGGTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTCCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCGCGTTCGCAGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGGCGCGGCCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATCCCCACGCCCGACGGCAAGGGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCGACCAACCTCGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTTTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCCACGCCGATTCCGCCAACGA
GGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCGCGCACGCTGGCGCAGGGCCTGGATGGCGTCGAGGTGGCCG
AGGCCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A518N2U7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.109

90.691

0.391
  pilT Pseudomonas aeruginosa PAK

40.708

90.16

0.367
  pilT Acinetobacter nosocomialis M2

40.597

89.096

0.362
  pilT Acinetobacter baumannii D1279779

40.597

89.096

0.362
  pilT Acinetobacter baumannii strain A118

40.597

89.096

0.362

Multiple sequence alignment