Detailed information NTG0375443

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: G9W35_RS09335
• Genome accession: NZ_CP049845
• Coordinates: 1935274..1936254 (+)
• Length: 326 a.a.
• NCBI ID: WP_001055620.1
• Uniprot ID: -
• Organism: Escherichia coli strain UPEC-245U/190328
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1930274..1941254

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G9W35_RS09305 (G9W35_09230)	-	1930649..1931635 (-)	987	WP_000784005.1	TRAP transporter substrate-binding protein	-
G9W35_RS09310 (G9W35_09235)	hemW	1931951..1933087 (-)	1137	WP_000239917.1	radical SAM family heme chaperone HemW	-
G9W35_RS09315 (G9W35_09240)	rdgB	1933080..1933673 (-)	594	WP_001174743.1	XTP/dITP diphosphatase	-
G9W35_RS09320 (G9W35_09245)	yggU	1933681..1933971 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
G9W35_RS09325 (G9W35_09250)	yggT	1933968..1934534 (-)	567	WP_238020652.1	osmotic shock tolerance protein YggT	-
G9W35_RS09330 (G9W35_09255)	yggS	1934552..1935256 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
G9W35_RS09335 (G9W35_09260)	pilT	1935274..1936254 (+)	981	WP_001055620.1	type IV pilus twitching motility protein PilT	Machinery gene
G9W35_RS09340 (G9W35_09265)	ruvX	1936430..1936846 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
G9W35_RS09345 (G9W35_09270)	yqgE	1936846..1937409 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
G9W35_RS09350 (G9W35_09275)	gshB	1937518..1938468 (-)	951	WP_074500088.1	glutathione synthase	-
G9W35_RS09355 (G9W35_09280)	rsmE	1938481..1939212 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
G9W35_RS09360 (G9W35_09285)	endA	1939292..1939999 (-)	708	WP_000286500.1	deoxyribonuclease I	-
G9W35_RS09365 (G9W35_09290)	yggI	1940094..1940591 (-)	498	WP_074500090.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35903.08 Da
• Isoelectric Point: 5.5860

>NTDB_id=375443 G9W35_RS09335 WP_001055620.1 1935274..1936254(+) (pilT) [Escherichia coli strain UPEC-245U/190328]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=375443 G9W35_RS09335 WP_001055620.1 1935274..1936254(+) (pilT) [Escherichia coli strain UPEC-245U/190328]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	37.879	100	0.383
pilU	Acinetobacter baylyi ADP1	37.346	99.387	0.371
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365