Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FPK91_RS19645 Genome accession   NZ_CP041783
Coordinates   4601250..4602287 (+) Length   345 a.a.
NCBI ID   WP_144213602.1    Uniprot ID   -
Organism   Shewanella donghaensis strain LT17     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4596250..4607287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPK91_RS19615 rdgB 4596841..4597440 (-) 600 WP_144213590.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FPK91_RS19620 - 4597715..4598149 (-) 435 WP_144213592.1 DUF4426 domain-containing protein -
  FPK91_RS19625 yggU 4598361..4598651 (-) 291 WP_144213594.1 DUF167 family protein YggU -
  FPK91_RS19630 - 4598713..4599264 (-) 552 WP_144213596.1 YggT family protein -
  FPK91_RS19635 proC 4599429..4600247 (-) 819 WP_144213598.1 pyrroline-5-carboxylate reductase -
  FPK91_RS19640 - 4600519..4601214 (-) 696 WP_144213600.1 YggS family pyridoxal phosphate-dependent enzyme -
  FPK91_RS19645 pilT 4601250..4602287 (+) 1038 WP_144213602.1 type IV pilus twitching motility protein PilT Machinery gene
  FPK91_RS19650 pilU 4602299..4603411 (+) 1113 WP_144213604.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FPK91_RS19655 - 4603590..4604711 (+) 1122 WP_144213606.1 hypothetical protein -
  FPK91_RS19660 - 4604876..4605421 (+) 546 WP_144213608.1 glutathione peroxidase -
  FPK91_RS19665 hemH 4605549..4606562 (+) 1014 WP_144213610.1 ferrochelatase -
  FPK91_RS19670 ruvX 4606698..4607120 (-) 423 WP_144213612.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38259.80 Da        Isoelectric Point: 6.7842

>NTDB_id=374255 FPK91_RS19645 WP_144213602.1 4601250..4602287(+) (pilT) [Shewanella donghaensis strain LT17]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQAVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAGAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINNERHDHILTIED
PIEFVHQNKQCLVNQREVHKHTHSFDAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTVDRI
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLEQCL
QNLVNRGLISRDDAMSKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=374255 FPK91_RS19645 WP_144213602.1 4601250..4602287(+) (pilT) [Shewanella donghaensis strain LT17]
ATGGAAATAACTGAGTTACTTGCCTTTAGTGTAAAACATAAAGCATCGGATCTACACCTTTCAGCAGGTGTTTCTCCAAT
GATTCGAGTTGATGGTGAAGTTAGAAAAATTAATTTACCCGCGTTAGATCACCAAGCTGTTCATGGTTTGGTCTATGACA
TCATGAATGATAAGCAGCGTAAGGACTACGAAGAACATCTAGAAATCGATTTCTCGTTTGAAGTGCCTAATCTAGCTCGA
TTCCGTGTGAATGCTTTTAACCAATCACGTGGTGCCGGTGCTGTTTTTCGTACAATTCCAAGTGATATCTTATCACTTGA
GCAATTAGGTGCCCCTGAAATATTCAAAAAGATTTCTAGCTTTCCCCGTGGTTTAGTTCTAGTAACAGGTCCTACGGGTT
CAGGTAAGTCGACCACATTAGCAGCCATGGTTGATTATATTAATAACGAACGCCATGACCATATTCTCACCATTGAAGAC
CCAATAGAATTCGTCCATCAAAATAAACAATGTCTGGTCAACCAACGTGAAGTACATAAGCATACTCACAGTTTCGATGC
TGCACTTCGAAGTGCGTTACGTGAAGATCCCGACGTTATTCTTGTTGGTGAAATGCGTGACCTTGAGACTATTCGTCTTG
CAATGACAGCGGCAGAAACAGGTCACTTAGTATTTGGTACCTTGCATACCACCTCAGCGGCTAAAACTGTTGACCGTATC
GTCGATGTATTCCCAGCTGGTGAAAAAGACATGGTTCGCACCATGTTATCTGAATCTTTACAGGCTGTTATTTCACAGAC
CTTGATTAAAAAGGTGGGCGGCGGCCGAGTTGCAGCACATGAAATTATGATGGGAACACCGGCTATTCGTAACCTTATTC
GTGAAGATAAAGTCGCGCAAATGTATTCAGCTATTCAAACCGGTATGTCACATGGCATGCAAACCCTTGAACAGTGCCTA
CAGAATCTAGTCAATCGCGGGCTTATTAGTCGTGATGATGCAATGTCAAAAAGCTCAAATAAACAAGCTAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.261

100

0.783
  pilT Acinetobacter nosocomialis M2

78.426

99.42

0.78
  pilT Acinetobacter baumannii D1279779

78.426

99.42

0.78
  pilT Acinetobacter baumannii strain A118

78.426

99.42

0.78
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

80

95.652

0.765
  pilT Legionella pneumophila strain Lp02

75.073

98.841

0.742
  pilT Legionella pneumophila strain ERS1305867

75.073

98.841

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

42.262

97.391

0.412
  pilU Acinetobacter baylyi ADP1

40.356

97.681

0.394

Multiple sequence alignment