Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FPK91_RS19650 Genome accession   NZ_CP041783
Coordinates   4602299..4603411 (+) Length   370 a.a.
NCBI ID   WP_144213604.1    Uniprot ID   -
Organism   Shewanella donghaensis strain LT17     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4597299..4608411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPK91_RS19620 - 4597715..4598149 (-) 435 WP_144213592.1 DUF4426 domain-containing protein -
  FPK91_RS19625 yggU 4598361..4598651 (-) 291 WP_144213594.1 DUF167 family protein YggU -
  FPK91_RS19630 - 4598713..4599264 (-) 552 WP_144213596.1 YggT family protein -
  FPK91_RS19635 proC 4599429..4600247 (-) 819 WP_144213598.1 pyrroline-5-carboxylate reductase -
  FPK91_RS19640 - 4600519..4601214 (-) 696 WP_144213600.1 YggS family pyridoxal phosphate-dependent enzyme -
  FPK91_RS19645 pilT 4601250..4602287 (+) 1038 WP_144213602.1 type IV pilus twitching motility protein PilT Machinery gene
  FPK91_RS19650 pilU 4602299..4603411 (+) 1113 WP_144213604.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FPK91_RS19655 - 4603590..4604711 (+) 1122 WP_144213606.1 hypothetical protein -
  FPK91_RS19660 - 4604876..4605421 (+) 546 WP_144213608.1 glutathione peroxidase -
  FPK91_RS19665 hemH 4605549..4606562 (+) 1014 WP_144213610.1 ferrochelatase -
  FPK91_RS19670 ruvX 4606698..4607120 (-) 423 WP_144213612.1 Holliday junction resolvase RuvX -
  FPK91_RS19675 - 4607242..4607802 (-) 561 WP_144213614.1 YqgE/AlgH family protein -
  FPK91_RS19680 yciH 4607893..4608222 (-) 330 WP_144214611.1 stress response translation initiation inhibitor YciH -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41278.25 Da        Isoelectric Point: 5.9372

>NTDB_id=374256 FPK91_RS19650 WP_144213604.1 4602299..4603411(+) (pilU) [Shewanella donghaensis strain LT17]
MDVRPFLKVMVERKASDLFVTAGFPPSAKIDGELRPLSDNNFSPEQSLEFVESLMTDVQKQEFHESRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLASLVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRIASLIAKNELHLLKETMGKSREQGMQTFDQA
LLDLYVEKEISYADALHHADSPNDLRLMIKLQNNDANSSGMMDGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=374256 FPK91_RS19650 WP_144213604.1 4602299..4603411(+) (pilU) [Shewanella donghaensis strain LT17]
ATGGATGTTCGTCCTTTTTTAAAGGTAATGGTTGAGCGTAAAGCATCAGATTTATTTGTCACCGCAGGTTTTCCACCTAG
TGCCAAAATCGATGGTGAACTTCGTCCGTTGTCAGACAATAATTTTAGCCCTGAGCAATCGTTAGAGTTTGTTGAGTCAT
TGATGACTGACGTTCAAAAGCAAGAATTTCACGAATCCCGTGAATGTAACTTTGCTTTTGCTGCAAAAGAGCTTGGCCGA
TTTCGTGTCAGTGCCTTTTGGCAGCGCGAATCTCCGGGTTGTGTTATGCGCCGCATTGAAACCAAAATACCTGAAGTTGA
TGACTTAAAACTGCCGCCTATCCTTAAAGATTTGGTGATGAGTAAACGTGGTTTAATCATCATGGTTGGTGGTACCGGTA
CCGGTAAATCAACCTCATTAGCATCACTAGTCGGTTATCGAAATGCCCATGCCCGTGGCCATATTCTCACGATTGAAGAT
CCTGTGGAATTTGTTCATGATCATCGCAAGAGTATTATTACTCAGCGGGAAGTCGGCATTGATACCGATTCGTTTGATGC
AGCACTAAAAAGTTCATTACGTCAGGCGCCTGATGTTATCTTGATTGGTGAGATCCGTACTCAAGAGACCATGGAGTTTG
CATTGTCGTTCGCTGAAACGGGTCACTTATGTATGGCAACATTGCATGCTAACAACGCTAACCAAGCATTAGACCGTATT
ATGCATCTTGTTCCAGAAAGTAAGCATAACCAGTTATTGTTCGATTTATCGTTAAACCTTCGCGGTATTGTGGCGCAGCA
ACTGGTGCCTAAATCCGATGGTTCAGGTCGACGAGCTGCCATTGAAGTGTTAATTAATACCCCACGTATCGCCAGTTTGA
TTGCCAAAAATGAACTGCATTTACTCAAAGAAACAATGGGTAAATCACGAGAGCAGGGCATGCAGACTTTTGACCAAGCA
TTATTAGATTTATACGTTGAAAAAGAGATTAGCTACGCTGATGCATTGCATCATGCTGACTCGCCAAATGACTTACGCTT
GATGATTAAATTACAAAATAATGATGCTAATTCATCTGGCATGATGGATGGTGTCACTTTAGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Acinetobacter baylyi ADP1

61.648

95.135

0.586
  pilU Vibrio cholerae strain A1552

57.027

100

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.87

93.243

0.381
  pilT Legionella pneumophila strain Lp02

40.708

91.622

0.373
  pilT Legionella pneumophila strain ERS1305867

40.708

91.622

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.26

86.216

0.373
  pilT Vibrio cholerae strain A1552

43.26

86.216

0.373
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362

Multiple sequence alignment