Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M301_RS03315 Genome accession   NC_014207
Coordinates   704597..705640 (+) Length   347 a.a.
NCBI ID   WP_013147346.1    Uniprot ID   -
Organism   Methylotenera versatilis 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 699597..710640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS03295 (M301_0642) - 700222..702294 (-) 2073 WP_013147342.1 dynamin family protein -
  M301_RS03300 (M301_0643) - 702309..702896 (-) 588 WP_013147343.1 YggT family protein -
  M301_RS03305 (M301_0644) proC 702880..703686 (-) 807 WP_013147344.1 pyrroline-5-carboxylate reductase -
  M301_RS03310 (M301_0645) - 703731..704453 (-) 723 WP_013147345.1 YggS family pyridoxal phosphate-dependent enzyme -
  M301_RS03315 (M301_0646) pilT 704597..705640 (+) 1044 WP_013147346.1 type IV pilus twitching motility protein PilT Machinery gene
  M301_RS03320 (M301_0647) pilU 705713..706849 (+) 1137 WP_013147347.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M301_RS03325 (M301_0648) - 706945..707730 (-) 786 WP_013147348.1 OmpA family protein -
  M301_RS03330 (M301_0649) - 707802..708353 (-) 552 WP_013147349.1 hypothetical protein -
  M301_RS03335 (M301_0650) - 708476..709753 (-) 1278 WP_013147350.1 dihydroorotase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38272.98 Da        Isoelectric Point: 6.5735

>NTDB_id=37383 M301_RS03315 WP_013147346.1 704597..705640(+) (pilT) [Methylotenera versatilis 301]
MDISDLLAFSVKNKASDLHLSAGLPPMIRVHGDIRKINVPALDHTAVHDMVYDIMSDGQRKVYEETLECDFSFEIPNLAR
FRVNAFNHQRGSGAVFRTIPSKILTLEELNCPAIFKDIADTPRGLCLVTGPTGSGKSTTLAAMIDYINDKEFGHILTVED
PIEFVHTSKKCLINQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLALTAAETGHMVFGTLHTSSAAKTIDRI
IDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDEQGRVAAHEIMIGTPAIRNLIREAKIPQMYSAIQTGQNVGMQTLDQN
LQDLVKRNVVSANEARAKAANKDSIMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=37383 M301_RS03315 WP_013147346.1 704597..705640(+) (pilT) [Methylotenera versatilis 301]
GTGGATATTTCAGATTTACTCGCATTCTCAGTTAAAAATAAAGCATCGGACTTACATCTTTCAGCTGGCTTGCCACCAAT
GATTCGTGTGCATGGCGACATTCGTAAGATTAATGTGCCGGCTTTAGATCATACCGCAGTGCATGACATGGTGTACGACA
TCATGAGCGATGGTCAGCGTAAAGTGTATGAAGAGACTTTGGAGTGTGACTTTTCGTTTGAAATTCCCAATTTGGCACGT
TTTCGGGTCAATGCTTTTAACCACCAGCGCGGTTCTGGCGCAGTGTTTCGTACCATTCCATCTAAAATATTGACATTAGA
AGAGCTTAATTGTCCTGCTATTTTTAAAGATATTGCTGACACGCCACGCGGACTTTGCTTGGTGACTGGGCCAACAGGCT
CGGGAAAGTCAACTACCTTGGCAGCGATGATTGATTACATTAATGATAAAGAATTCGGTCACATTTTGACGGTAGAAGAC
CCGATTGAGTTTGTGCATACTTCTAAGAAGTGTTTAATCAACCAGCGTGAAGTTGGACCACATACCCTGTCATTTAATAA
CGCTTTACGCTCCGCATTACGTGAGGATCCTGATGTGATTCTAGTGGGTGAAATGCGTGACCTTGAAACGATACGTTTGG
CGCTTACTGCGGCAGAAACTGGCCACATGGTGTTTGGTACACTACATACCAGTTCAGCAGCTAAAACTATTGACCGTATT
ATTGACGTTTTTCCTGCCGCTGAAAAAGAAATGGTGCGATCAATGTTATCCGAATCCTTACGCGCTGTTATTTCACAAAC
TTTATTGAAAACTAAAGATGAACAGGGCCGCGTTGCCGCTCATGAAATCATGATTGGTACCCCTGCGATTCGTAACTTGA
TTCGCGAAGCTAAGATCCCACAAATGTATTCAGCTATTCAGACTGGTCAAAATGTGGGTATGCAAACGTTAGATCAAAAC
TTACAAGATTTAGTAAAACGTAATGTGGTTTCTGCGAATGAGGCACGTGCAAAAGCTGCGAATAAAGATTCCATTATGGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.434

99.712

0.752
  pilT Acinetobacter baumannii strain A118

75.434

99.712

0.752
  pilT Acinetobacter nosocomialis M2

75.434

99.712

0.752
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Pseudomonas aeruginosa PAK

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.099

98.559

0.72
  pilT Legionella pneumophila strain Lp02

73.099

98.559

0.72
  pilT Pseudomonas stutzeri DSM 10701

71.884

99.424

0.715
  pilT Neisseria meningitidis 8013

70.145

99.424

0.697
  pilT Neisseria gonorrhoeae MS11

69.855

99.424

0.695
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.935

97.406

0.671
  pilT Vibrio cholerae strain A1552

68.935

97.406

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.327

97.695

0.501
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Vibrio cholerae strain A1552

41.092

100

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment