Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M301_RS03320 Genome accession   NC_014207
Coordinates   705713..706849 (+) Length   378 a.a.
NCBI ID   WP_013147347.1    Uniprot ID   -
Organism   Methylotenera versatilis 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700713..711849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M301_RS03300 (M301_0643) - 702309..702896 (-) 588 WP_013147343.1 YggT family protein -
  M301_RS03305 (M301_0644) proC 702880..703686 (-) 807 WP_013147344.1 pyrroline-5-carboxylate reductase -
  M301_RS03310 (M301_0645) - 703731..704453 (-) 723 WP_013147345.1 YggS family pyridoxal phosphate-dependent enzyme -
  M301_RS03315 (M301_0646) pilT 704597..705640 (+) 1044 WP_013147346.1 type IV pilus twitching motility protein PilT Machinery gene
  M301_RS03320 (M301_0647) pilU 705713..706849 (+) 1137 WP_013147347.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M301_RS03325 (M301_0648) - 706945..707730 (-) 786 WP_013147348.1 OmpA family protein -
  M301_RS03330 (M301_0649) - 707802..708353 (-) 552 WP_013147349.1 hypothetical protein -
  M301_RS03335 (M301_0650) - 708476..709753 (-) 1278 WP_013147350.1 dihydroorotase -
  M301_RS03340 (M301_0651) - 709750..710730 (-) 981 WP_013147351.1 aspartate carbamoyltransferase catalytic subunit -
  M301_RS03345 (M301_0652) pyrR 710723..711280 (-) 558 WP_013147352.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  M301_RS03350 (M301_0653) ruvX 711329..711823 (-) 495 WP_013147353.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41960.20 Da        Isoelectric Point: 6.5054

>NTDB_id=37384 M301_RS03320 WP_013147347.1 705713..706849(+) (pilU) [Methylotenera versatilis 301]
MEKGQAEKFVFDLLRLMLAKNASDLFITAGFPPAMKIDGKVTPVSSQVLSAQQAREISRSIMNDKQTSEFDATNECNFAI
GIPSVARFRVNAFVQRGSVGLVFRTITTKIPEFEDLGLPEVLKDIAMTKRGLVIFVGGTGSGKSTSLAAMVGYRNKNSYG
HIITIEDPVEFVHEHINCIVTQREIGVDTDNWHIALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANST
NQALDRIINFFPEERRHQLLMDLSLNTRAFISQRLIPKKEGKGRAAAMEVMLNSPLISDLIFKGDVHEIKSIIAKSRELG
MQTFDQALFDLHEADVITYEDTLRNADSVNDIRLKIKLDGKASHGKDLSQGLDSLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=37384 M301_RS03320 WP_013147347.1 705713..706849(+) (pilU) [Methylotenera versatilis 301]
ATGGAAAAAGGTCAGGCAGAGAAGTTTGTATTTGATTTATTGAGGTTGATGCTTGCAAAAAATGCATCAGACTTATTTAT
CACCGCTGGTTTCCCACCAGCCATGAAAATAGATGGCAAAGTAACACCAGTGAGCAGCCAGGTGTTATCAGCACAGCAGG
CGCGTGAAATTTCACGCTCTATTATGAATGATAAACAAACCTCCGAGTTTGATGCGACTAATGAGTGTAATTTTGCTATT
GGTATTCCAAGTGTAGCGCGCTTTCGTGTGAATGCTTTCGTCCAGCGTGGTTCTGTGGGCTTGGTATTTAGAACGATTAC
TACAAAAATTCCAGAGTTTGAAGATTTAGGTCTACCTGAAGTGTTAAAAGATATTGCCATGACCAAACGTGGTTTGGTGA
TTTTTGTTGGTGGTACTGGTTCAGGTAAGTCGACTTCTTTAGCGGCAATGGTTGGCTATCGCAATAAAAATAGCTACGGT
CATATCATTACCATTGAAGATCCCGTGGAATTTGTGCATGAACATATCAACTGCATTGTCACCCAGCGCGAAATTGGTGT
GGATACGGATAACTGGCATATCGCACTTAAAAACACTCTGCGCCAGGCGCCAGACGTGATTTTGATTGGTGAGATTCGCG
ACCGTGAAACGATGGATTATGCGATTGCTTTCGCTGAAACGGGTCACTTATGCATGGCGACATTGCATGCAAATAGTACT
AATCAGGCCTTGGACCGTATTATTAACTTCTTCCCTGAAGAACGCCGCCATCAGTTGCTGATGGATTTATCATTAAATAC
ACGTGCCTTTATTTCACAACGACTGATTCCTAAAAAAGAGGGCAAAGGGCGTGCTGCTGCGATGGAGGTGATGTTGAATT
CACCGTTAATTTCAGATTTAATCTTTAAGGGTGATGTGCATGAAATTAAATCTATCATTGCCAAATCACGCGAGTTGGGT
ATGCAAACCTTTGACCAAGCGCTGTTTGATTTGCATGAGGCGGATGTGATTACATATGAAGATACCTTACGTAACGCTGA
CTCGGTGAATGATATTCGACTCAAAATTAAGTTGGATGGAAAAGCTTCACACGGAAAAGACTTATCGCAGGGGCTTGATA
GTCTTGGAATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.431

95.767

0.598
  pilU Acinetobacter baylyi ADP1

59.384

94.444

0.561
  pilU Vibrio cholerae strain A1552

56.305

90.212

0.508
  pilT Vibrio cholerae strain A1552

42.776

93.386

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.776

93.386

0.399
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Acinetobacter baumannii strain A118

44.214

89.153

0.394
  pilT Acinetobacter baumannii D1279779

44.214

89.153

0.394
  pilT Acinetobacter nosocomialis M2

44.214

89.153

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Pseudomonas stutzeri DSM 10701

45.455

84.392

0.384
  pilT Acinetobacter baylyi ADP1

44.099

85.185

0.376
  pilT Legionella pneumophila strain ERS1305867

42.553

87.037

0.37
  pilT Legionella pneumophila strain Lp02

42.553

87.037

0.37

Multiple sequence alignment