Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FNU76_RS05810 Genome accession   NZ_CP041730
Coordinates   1310163..1311203 (+) Length   346 a.a.
NCBI ID   WP_143856828.1    Uniprot ID   A0A516SCM5
Organism   Chitinimonas arctica strain R3-44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1305163..1316203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNU76_RS05780 (FNU76_05780) - 1306232..1306720 (+) 489 WP_143856822.1 FxsA family protein -
  FNU76_RS05785 (FNU76_05785) - 1306674..1306979 (-) 306 WP_143856823.1 DUF167 family protein -
  FNU76_RS05790 (FNU76_05790) - 1306986..1307570 (-) 585 WP_143856824.1 YggT family protein -
  FNU76_RS05795 (FNU76_05795) proC 1307626..1308453 (-) 828 WP_143856825.1 pyrroline-5-carboxylate reductase -
  FNU76_RS05800 (FNU76_05800) - 1308450..1309019 (-) 570 WP_143856826.1 hypothetical protein -
  FNU76_RS05805 (FNU76_05805) - 1309016..1309738 (-) 723 WP_143856827.1 YggS family pyridoxal phosphate-dependent enzyme -
  FNU76_RS05810 (FNU76_05810) pilT 1310163..1311203 (+) 1041 WP_143856828.1 type IV pilus twitching motility protein PilT Machinery gene
  FNU76_RS05815 (FNU76_05815) pilU 1311245..1312381 (+) 1137 WP_143856829.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FNU76_RS05820 (FNU76_05820) - 1312415..1313920 (-) 1506 WP_179958366.1 mechanosensitive ion channel family protein -
  FNU76_RS05825 (FNU76_05825) - 1314021..1315289 (+) 1269 WP_143856831.1 EAL and HDOD domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38383.12 Da        Isoelectric Point: 6.8812

>NTDB_id=373586 FNU76_RS05810 WP_143856828.1 1310163..1311203(+) (pilT) [Chitinimonas arctica strain R3-44]
MEISELLAFSVKNKASDLHLSSGLPPCIRVHGDVRRINLPPMEHKDVHDMVYDIMNDGQRKTYEETLECDFSFEIPGLAR
FRVNAFVQNRGAGAVFRTIPSKVLSLEELNAPKVFKEIAETPRGIVLVTGPTGSGKSTTLAAMVNYINENEYGHILTVED
PIEFVHESKKCLVNQREVGPHTKSFANSLRAALREDPDVILVGEMRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGTGRVAAHEIMIGIPAIRNLIRENKIAQMYSTIQTGQQHGMQTLDQC
LTELIRRNLITPAEARGKAMNPDNFK

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=373586 FNU76_RS05810 WP_143856828.1 1310163..1311203(+) (pilT) [Chitinimonas arctica strain R3-44]
ATGGAAATCTCGGAACTCCTGGCCTTCTCGGTCAAGAACAAGGCCTCGGACTTGCACCTGTCATCCGGCTTGCCACCCTG
CATCCGGGTGCACGGCGACGTACGGCGCATCAATCTGCCGCCGATGGAGCACAAGGACGTGCACGACATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGACCTACGAAGAAACCCTGGAGTGCGACTTTTCCTTCGAAATCCCCGGCCTGGCCCGT
TTCCGGGTCAATGCCTTCGTGCAGAACCGGGGTGCCGGCGCGGTATTCCGTACCATTCCGTCCAAGGTGCTGTCCCTGGA
GGAACTGAACGCCCCCAAGGTATTCAAGGAAATCGCCGAAACCCCTCGCGGCATCGTACTGGTGACCGGCCCGACCGGCT
CGGGTAAATCCACCACCCTGGCCGCGATGGTCAATTACATCAACGAGAATGAATACGGCCATATCCTGACCGTGGAAGAC
CCGATCGAATTCGTGCACGAATCGAAAAAATGCCTGGTCAACCAGCGTGAGGTCGGCCCTCATACCAAGAGTTTCGCCAA
CTCCCTGCGCGCGGCGCTGCGGGAAGACCCGGACGTTATCCTGGTGGGTGAAATGCGTGACCTGGAAACCATTCGGCTAG
CGCTGTCCGCAGCCGAAACAGGTCACTTGGTATTCGGCACCCTGCACACCTCGTCCGCGGCAAAGACCATCGACCGTATC
GTGGACGTATTCCCGGCGGCGGAAAAGGAAATGGTCCGTTCGATGCTGTCCGAATCGCTGCGCGCGGTCATCTCCCAGAC
CCTGCTCAAGACCAAGGACGGCACCGGCCGGGTCGCCGCGCACGAAATCATGATCGGCATTCCCGCCATCCGTAACCTGA
TCCGGGAAAACAAGATCGCGCAGATGTATTCGACCATTCAGACCGGCCAGCAGCACGGCATGCAGACGCTGGATCAGTGC
CTGACCGAATTGATACGCCGCAACCTCATCACCCCTGCGGAAGCCCGTGGCAAGGCGATGAACCCAGACAACTTCAAATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A516SCM5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.522

99.711

0.763
  pilT Acinetobacter baumannii D1279779

76.522

99.711

0.763
  pilT Acinetobacter baumannii strain A118

76.522

99.711

0.763
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.711

0.754
  pilT Pseudomonas aeruginosa PAK

75.652

99.711

0.754
  pilT Acinetobacter baylyi ADP1

74.203

99.711

0.74
  pilT Neisseria meningitidis 8013

72.174

99.711

0.72
  pilT Neisseria gonorrhoeae MS11

71.884

99.711

0.717
  pilT Legionella pneumophila strain Lp02

71.884

99.711

0.717
  pilT Legionella pneumophila strain ERS1305867

71.884

99.711

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.864

97.688

0.653
  pilT Vibrio cholerae strain A1552

66.864

97.688

0.653
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.711

0.509
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.821

0.413
  pilU Vibrio cholerae strain A1552

40.751

100

0.408
  pilU Acinetobacter baylyi ADP1

40.058

100

0.402

Multiple sequence alignment