Detailed information NTG0372978

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: G6Z98_RS00445
• Genome accession: NZ_CP049197
• Coordinates: 101269..102249 (+)
• Length: 326 a.a.
• NCBI ID: WP_001327409.1
• Uniprot ID: -
• Organism: Escherichia coli strain E597
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 96269..107249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G6Z98_RS00415	yggM	96870..97877 (+)	1008	WP_000745192.1	DUF1202 family protein	-
G6Z98_RS00420	hemW	97946..99082 (-)	1137	WP_000239981.1	radical SAM family heme chaperone HemW	-
G6Z98_RS00425	rdgB	99075..99668 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
G6Z98_RS00430	yggU	99676..99966 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
G6Z98_RS00435	yggT	99963..100529 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
G6Z98_RS00440	yggS	100547..101251 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
G6Z98_RS00445	pilT	101269..102249 (+)	981	WP_001327409.1	type IV pilus twitching motility protein PilT	Machinery gene
G6Z98_RS00450	ruvX	102423..102839 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
G6Z98_RS00455	yqgE	102839..103402 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
G6Z98_RS00460	gshB	103511..104461 (-)	951	WP_000593261.1	glutathione synthase	-
G6Z98_RS00465	rsmE	104474..105205 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
G6Z98_RS00470	endA	105285..105992 (-)	708	WP_001327408.1	deoxyribonuclease I	-
G6Z98_RS00475	yggI	106087..106584 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36089.34 Da
• Isoelectric Point: 5.9975

>NTDB_id=372978 G6Z98_RS00445 WP_001327409.1 101269..102249(+) (pilT) [Escherichia coli strain E597]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDALDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=372978 G6Z98_RS00445 WP_001327409.1 101269..102249(+) (pilT) [Escherichia coli strain E597]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCTGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.379	97.239	0.393
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365