Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FNC98_RS03085 Genome accession   NZ_CP041638
Coordinates   711825..712868 (+) Length   347 a.a.
NCBI ID   WP_143579887.1    Uniprot ID   -
Organism   Thalassotalea sp. PS06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 706825..717868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNC98_RS03060 (FNC98_03060) - 707965..708564 (-) 600 WP_144035433.1 XTP/dITP diphosphatase -
  FNC98_RS03065 (FNC98_03065) - 708669..709112 (-) 444 WP_143579883.1 DUF4426 domain-containing protein -
  FNC98_RS03070 (FNC98_03070) - 709619..710167 (-) 549 WP_143579884.1 YggT family protein -
  FNC98_RS03075 (FNC98_03075) proC 710221..711051 (-) 831 WP_143579885.1 pyrroline-5-carboxylate reductase -
  FNC98_RS03080 (FNC98_03080) - 711096..711782 (-) 687 WP_143579886.1 YggS family pyridoxal phosphate-dependent enzyme -
  FNC98_RS03085 (FNC98_03085) pilT 711825..712868 (+) 1044 WP_143579887.1 type IV pilus twitching motility protein PilT Machinery gene
  FNC98_RS03090 (FNC98_03090) pilU 712883..714004 (+) 1122 WP_143579888.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FNC98_RS03095 (FNC98_03095) metK 714073..715263 (-) 1191 WP_143579889.1 methionine adenosyltransferase -
  FNC98_RS03100 (FNC98_03100) rsmE 715895..716647 (+) 753 WP_143579890.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FNC98_RS03105 (FNC98_03105) gshB 716738..717700 (+) 963 WP_143579891.1 glutathione synthase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38693.27 Da        Isoelectric Point: 5.8141

>NTDB_id=372677 FNC98_RS03085 WP_143579887.1 711825..712868(+) (pilT) [Thalassotalea sp. PS06]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKVNVPALEDKDVNALVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSEILSLDQLGCPDIFRSISENPRGLVLVTGPTGSGKSTTLAAMVDYINDTKRHHILTIED
PIEFVHPNKQCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMQHGMQTMDQCL
QDLLSRGVISREDAMSKAADKNQFKVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=372677 FNC98_RS03085 WP_143579887.1 711825..712868(+) (pilT) [Thalassotalea sp. PS06]
ATGGATATTACCGAACTATTAGCATTTAGTGTCGAACATAATGCATCCGATTTACATCTATCTACAGGCATGCCCCCCGC
CATTCGAGTAGACGGTGATGTACGTAAAGTTAACGTACCCGCCCTTGAAGATAAAGACGTCAACGCGCTGGTTTACGATA
TTATGACCGATCGCCAGCGAAAAGAATACGAAGAAAATTTGGAAGTGGATTTCTCCTTTGAAGTGCCAAACCTGGCGCGT
TTCAGGGTAAATGCATTCAACCAGAATCGTGGGCCTGCTGCGGTATTCCGTACCATTCCAAGTGAAATATTATCTTTGGA
CCAATTAGGGTGTCCGGATATTTTCCGTTCTATTTCCGAAAATCCTCGTGGCCTGGTGCTGGTTACAGGACCGACTGGTT
CTGGTAAATCAACAACGCTGGCGGCGATGGTCGATTACATTAATGACACCAAACGTCATCACATTTTAACCATCGAAGAT
CCAATCGAATTTGTGCATCCAAACAAGCAATGTTTGATTAACCAACGTGAAGTTCATCGGGATACCCTGAGCTTTAACAA
CGCTCTGCGTTCAGCACTTCGTGAAGATCCAGATGTAATTCTGGTTGGTGAGATGCGTGACCTTGAAACCATTCGCCTGG
CAATGACCGCAGCAGAAACCGGTCACTTGGTTTTTGGCACCTTGCACACCACCTCCGCACCGAAAACCATTGACCGTATT
ATCGATGTATTCCCAGCTGCGGAAAAAGATATGGTGCGTTCAATGCTTTCTGAATCGTTACGCGCAGTAATTTCGCAAAC
GCTGATCAAGAAAGTGGGTGGTGGTCGAGTTGCTGCTCATGAAATTATGATTGGTGTTCCAGCGATTCGTAATTTGATTC
GTGAAGATAAGATTGCACAAATGTACTCGGCGATTCAGACCGGTATGCAACATGGTATGCAAACGATGGATCAGTGTCTG
CAGGACTTATTGAGCCGTGGTGTGATTTCCCGCGAGGATGCAATGTCTAAAGCCGCCGATAAAAATCAGTTTAAAGTTTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.744

99.135

0.761
  pilT Acinetobacter baumannii D1279779

76.453

99.135

0.758
  pilT Acinetobacter baumannii strain A118

76.453

99.135

0.758
  pilT Pseudomonas stutzeri DSM 10701

76.163

99.135

0.755
  pilT Pseudomonas aeruginosa PAK

76.163

99.135

0.755
  pilT Acinetobacter baylyi ADP1

75.291

99.135

0.746
  pilT Legionella pneumophila strain Lp02

72.965

99.135

0.723
  pilT Legionella pneumophila strain ERS1305867

72.965

99.135

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.118

0.703
  pilT Vibrio cholerae strain A1552

72.404

97.118

0.703
  pilT Neisseria meningitidis 8013

68.622

98.271

0.674
  pilT Neisseria gonorrhoeae MS11

68.328

98.271

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Vibrio cholerae strain A1552

40.922

100

0.409
  pilU Acinetobacter baylyi ADP1

40.517

100

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.058

100

0.401

Multiple sequence alignment