Detailed information NTG0372678

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FNC98_RS03090
• Genome accession: NZ_CP041638
• Coordinates: 712883..714004 (+)
• Length: 373 a.a.
• NCBI ID: WP_143579888.1
• Uniprot ID: A0A516H9I6
• Organism: Thalassotalea sp. PS06
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 707883..719004

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FNC98_RS03060 (FNC98_03060)	-	707965..708564 (-)	600	WP_144035433.1	XTP/dITP diphosphatase	-
FNC98_RS03065 (FNC98_03065)	-	708669..709112 (-)	444	WP_143579883.1	DUF4426 domain-containing protein	-
FNC98_RS03070 (FNC98_03070)	-	709619..710167 (-)	549	WP_143579884.1	YggT family protein	-
FNC98_RS03075 (FNC98_03075)	proC	710221..711051 (-)	831	WP_143579885.1	pyrroline-5-carboxylate reductase	-
FNC98_RS03080 (FNC98_03080)	-	711096..711782 (-)	687	WP_143579886.1	YggS family pyridoxal phosphate-dependent enzyme	-
FNC98_RS03085 (FNC98_03085)	pilT	711825..712868 (+)	1044	WP_143579887.1	type IV pilus twitching motility protein PilT	Machinery gene
FNC98_RS03090 (FNC98_03090)	pilU	712883..714004 (+)	1122	WP_143579888.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FNC98_RS03095 (FNC98_03095)	metK	714073..715263 (-)	1191	WP_143579889.1	methionine adenosyltransferase	-
FNC98_RS03100 (FNC98_03100)	rsmE	715895..716647 (+)	753	WP_143579890.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
FNC98_RS03105 (FNC98_03105)	gshB	716738..717700 (+)	963	WP_143579891.1	glutathione synthase	-
FNC98_RS03110 (FNC98_03110)	-	717763..718317 (+)	555	WP_143579892.1	YqgE/AlgH family protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41230.23 Da
• Isoelectric Point: 5.0627

>NTDB_id=372678 FNC98_RS03090 WP_143579888.1 712883..714004(+) (pilU) [Thalassotalea sp. PS06]
MYFHQLLQTMIEKDGSDLFITVNLPPSAKINGELTPIDDQVMNYGSALELVEGSMNEKQLKEYEETKECNYAIAVDGIGR
FRISAFWQREMPGMVIRRIVTEIPDVDKLGLPQVLKDVVMSKRGLVLFVGGTGTGKSTSMASLIGYRNRNARGHILTIED
PVEFVHEHGKSMITQREVGMDTESFDAALKSSLRQAPDVILIGEIRSQEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHGKLLFDLALNLRGIIAQQLVPTRDGNGRAAAIEILLNSPYIAELIKKGEVGDIKEVMAKSRELGMQTFDQA
LFDLYEAGKISYTDALHHADSPNDLRLMIKLRSNEQTGMGGLKGATLDGLDDD

Nucleotide

• Length: 1122 bp

>NTDB_id=372678 FNC98_RS03090 WP_143579888.1 712883..714004(+) (pilU) [Thalassotalea sp. PS06]
ATGTATTTTCATCAATTACTGCAAACCATGATTGAAAAGGATGGTTCCGATTTATTCATTACCGTAAACCTGCCACCAAG
TGCAAAAATCAATGGTGAATTAACACCGATTGATGACCAGGTGATGAACTATGGTTCTGCACTTGAGCTTGTTGAAGGTT
CAATGAATGAAAAGCAGTTGAAGGAATATGAAGAAACCAAAGAGTGTAACTATGCGATAGCGGTTGATGGTATTGGCCGT
TTTCGTATCTCAGCATTCTGGCAACGAGAAATGCCGGGGATGGTTATCCGTCGTATCGTTACCGAAATTCCTGATGTAGA
TAAGCTTGGTTTACCACAGGTCCTAAAAGATGTGGTGATGTCAAAGCGCGGTCTGGTGTTATTCGTAGGTGGTACCGGTA
CCGGTAAATCAACCTCGATGGCATCTTTGATTGGTTATCGAAACCGTAATGCCCGTGGCCACATTCTTACTATCGAGGAT
CCGGTAGAATTTGTTCACGAGCACGGTAAGTCAATGATCACCCAACGGGAAGTAGGGATGGATACTGAGTCGTTTGACGC
GGCACTGAAAAGTTCCCTACGTCAGGCACCTGACGTTATCCTGATTGGTGAAATCCGTTCTCAGGAGATCATGGAGCATG
CCCTGAGTTTCGCAGAAACTGGTCATTTGTGTATTGCTACACTCCACGCCAACAACGCTAACCAGGCGATAGACCGTATT
ATGCACCTGGTACCAGCTGAGCAGCATGGTAAGTTGTTATTCGACTTAGCATTAAACCTGCGCGGTATCATCGCTCAACA
ACTGGTGCCAACGCGTGACGGTAATGGCCGGGCTGCGGCTATCGAGATCCTTTTAAATTCTCCTTATATCGCTGAATTGA
TTAAGAAGGGTGAAGTAGGGGATATCAAAGAGGTGATGGCAAAATCTCGTGAGCTGGGTATGCAAACCTTTGACCAGGCA
TTATTTGACCTGTATGAGGCGGGCAAAATCAGTTACACCGATGCGTTGCATCATGCCGATTCACCGAACGATCTGCGTTT
GATGATCAAACTTCGCTCTAACGAGCAGACCGGTATGGGCGGACTTAAAGGCGCCACCCTTGATGGCCTTGACGACGATT
AA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: A0A516H9I6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.574	100	0.601
pilU	Acinetobacter baylyi ADP1	58.807	94.37	0.555
pilU	Vibrio cholerae strain A1552	55.163	98.66	0.544
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.292	95.442	0.394
pilT	Pseudomonas aeruginosa PAK	39.13	92.493	0.362