Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FLM21_RS08030 Genome accession   NZ_CP041335
Coordinates   1645209..1646252 (+) Length   347 a.a.
NCBI ID   WP_148715076.1    Uniprot ID   -
Organism   Chitinolyticbacter meiyuanensis strain SYBC-H1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1640209..1651252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM21_RS07995 dsbD 1640442..1641980 (-) 1539 WP_148715069.1 protein-disulfide reductase DsbD -
  FLM21_RS08000 cutA 1641983..1642315 (-) 333 WP_148715070.1 divalent-cation tolerance protein CutA -
  FLM21_RS08005 - 1642314..1642721 (+) 408 WP_187360139.1 FxsA family protein -
  FLM21_RS08010 - 1642711..1642995 (-) 285 WP_148715072.1 DUF167 domain-containing protein -
  FLM21_RS08015 - 1642992..1643585 (-) 594 WP_148715073.1 YggT family protein -
  FLM21_RS08020 proC 1643595..1644410 (-) 816 WP_148715074.1 pyrroline-5-carboxylate reductase -
  FLM21_RS08025 - 1644427..1645113 (-) 687 WP_148715075.1 YggS family pyridoxal phosphate-dependent enzyme -
  FLM21_RS08030 pilT 1645209..1646252 (+) 1044 WP_148715076.1 type IV pilus twitching motility protein PilT Machinery gene
  FLM21_RS08035 pilU 1646314..1647450 (+) 1137 WP_148715077.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FLM21_RS08040 - 1647524..1649527 (+) 2004 WP_187360140.1 FimV family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38303.95 Da        Isoelectric Point: 6.6672

>NTDB_id=371307 FLM21_RS08030 WP_148715076.1 1645209..1646252(+) (pilT) [Chitinolyticbacter meiyuanensis strain SYBC-H1]
MEISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHDMVYDIMNDGQRKIYEDTLEADFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLTLEELNAPKVFQDISENPRGLVLVTGPTGSGKSTTLAAMVNYINENEYGHILTVED
PIEFVHQSKKCLINQREVGPHTLSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKEMVRAMLSESLRAVISQTLLKTKDGNGRVAAHEIMIGIPAIRNLIRENKIAQMYSAIQTGSQFGMQTLDQG
LQTLVQRNVVSLAEARTKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=371307 FLM21_RS08030 WP_148715076.1 1645209..1646252(+) (pilT) [Chitinolyticbacter meiyuanensis strain SYBC-H1]
ATGGAAATCTCCGAACTCTTGGCTTTCTCCGTCAAGAACAAGGCCTCCGACTTGCACCTGTCCGCCGGCTTGCCGCCGAT
GATCCGCGTCCATGGCGACGTACGCCGTATCAATCTGCCGCCCTTGGAGCACAAGGACGTACACGACATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAGATATACGAGGACACGCTTGAGGCCGACTTCTCGTTCGAGATCCCCAACCTGGCGCGT
TTCCGGGTCAATGCCTTCGTGCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCGTCCAAGGTGCTGACGCTCGA
AGAGCTCAACGCCCCCAAGGTATTCCAGGACATCTCGGAGAACCCGCGTGGCCTGGTGCTGGTGACGGGGCCTACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGGTCAATTACATCAACGAGAATGAGTACGGTCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACCAGTCCAAGAAGTGCTTGATCAACCAACGCGAGGTTGGTCCGCATACGCTGAGTTTCTCCAA
TGCGCTGCGCTCCGCGCTGCGGGAGGATCCGGACGTGATCCTGGTCGGTGAAATGCGTGACCTCGAAACCATTCGCCTGG
CACTGACCGCCGCGGAAACGGGCCACTTGGTGTTCGGCACGCTGCATACCAGCTCGGCCGCCAAGACCATCGACCGCGTG
GTCGACGTATTCCCGGCGGCGGAAAAGGAAATGGTGCGCGCGATGTTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
GCTGCTCAAGACCAAGGACGGTAACGGCCGCGTGGCGGCGCACGAGATCATGATCGGCATCCCCGCCATCCGGAACCTGA
TCCGCGAGAACAAGATCGCCCAGATGTATTCGGCGATCCAGACCGGCAGCCAGTTCGGCATGCAGACGCTCGATCAGGGC
CTGCAGACCCTGGTTCAGCGCAATGTCGTGTCGCTGGCCGAGGCCCGCACCAAGGCCGCGATTCCGGACAATTTCAAGGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80

99.424

0.795
  pilT Acinetobacter baumannii D1279779

80

99.424

0.795
  pilT Acinetobacter baumannii strain A118

80

99.424

0.795
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Acinetobacter baylyi ADP1

75.942

99.424

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Neisseria meningitidis 8013

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

73.156

97.695

0.715
  pilT Legionella pneumophila strain Lp02

73.716

95.389

0.703
  pilT Legionella pneumophila strain ERS1305867

73.716

95.389

0.703
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.343

97.406

0.666
  pilT Vibrio cholerae strain A1552

68.343

97.406

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Acinetobacter baylyi ADP1

38.617

100

0.386

Multiple sequence alignment