Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FLM21_RS08035 Genome accession   NZ_CP041335
Coordinates   1646314..1647450 (+) Length   378 a.a.
NCBI ID   WP_148715077.1    Uniprot ID   -
Organism   Chitinolyticbacter meiyuanensis strain SYBC-H1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1641314..1652450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM21_RS08000 cutA 1641983..1642315 (-) 333 WP_148715070.1 divalent-cation tolerance protein CutA -
  FLM21_RS08005 - 1642314..1642721 (+) 408 WP_187360139.1 FxsA family protein -
  FLM21_RS08010 - 1642711..1642995 (-) 285 WP_148715072.1 DUF167 domain-containing protein -
  FLM21_RS08015 - 1642992..1643585 (-) 594 WP_148715073.1 YggT family protein -
  FLM21_RS08020 proC 1643595..1644410 (-) 816 WP_148715074.1 pyrroline-5-carboxylate reductase -
  FLM21_RS08025 - 1644427..1645113 (-) 687 WP_148715075.1 YggS family pyridoxal phosphate-dependent enzyme -
  FLM21_RS08030 pilT 1645209..1646252 (+) 1044 WP_148715076.1 type IV pilus twitching motility protein PilT Machinery gene
  FLM21_RS08035 pilU 1646314..1647450 (+) 1137 WP_148715077.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FLM21_RS08040 - 1647524..1649527 (+) 2004 WP_187360140.1 FimV family protein -
  FLM21_RS08045 - 1649589..1651475 (-) 1887 WP_148715079.1 MFS transporter -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42237.36 Da        Isoelectric Point: 6.4347

>NTDB_id=371308 FLM21_RS08035 WP_148715077.1 1646314..1647450(+) (pilU) [Chitinolyticbacter meiyuanensis strain SYBC-H1]
MEKEQAAKFMQDLLRHMRSKGASDLFITADFPPAMKIDGRVTPVSAQVLTAQHTKELARSIMNDRQAEEFEASKECNFAI
SPGNLGRFRVNTFMQQSRVGMVLRTINSEIPKLTELGLPPVLQDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNESSYD
HIITIEDPIEYVHEHKNCIVTQREVGVDTDSWGAALKNTLRQAPDVILIGEIRDRDTMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRAQLLMDLSLNLKAFVSQRLVPHRSGKGRVAAVEVMLNSPLISDLIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLYEAGKISYEDALRNADSVNDLRLQIKLQGSESKQRDVLSGIDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=371308 FLM21_RS08035 WP_148715077.1 1646314..1647450(+) (pilU) [Chitinolyticbacter meiyuanensis strain SYBC-H1]
ATGGAAAAAGAGCAGGCAGCCAAATTCATGCAAGACCTGCTGCGCCACATGCGCAGCAAGGGAGCCTCGGACCTGTTCAT
CACCGCGGATTTCCCGCCGGCGATGAAGATCGACGGCCGGGTCACGCCGGTGTCGGCGCAGGTGCTGACCGCCCAGCACA
CCAAGGAGCTGGCGCGTTCCATCATGAACGATCGCCAGGCTGAGGAGTTCGAGGCCAGCAAGGAGTGTAACTTTGCGATC
AGCCCCGGTAATCTCGGGCGCTTTCGCGTCAATACCTTCATGCAGCAAAGCCGCGTCGGCATGGTGTTGCGGACCATCAA
TTCCGAAATCCCCAAGCTGACCGAGCTTGGCCTGCCGCCGGTGCTGCAGGACATTGCGATGACCAAGCGTGGGTTGGTGA
TCTTTGTCGGCGGCACGGGCTCGGGCAAGTCGACCTCGCTCGCCGCCATGATCGGCCACCGCAATGAGAGCTCGTACGAT
CACATCATCACCATCGAAGACCCGATTGAGTATGTGCACGAGCACAAGAACTGCATCGTCACCCAGCGTGAAGTGGGCGT
CGATACCGACAGCTGGGGCGCGGCACTGAAGAACACGCTGCGCCAGGCCCCAGACGTGATCCTGATCGGTGAGATCCGTG
ATCGTGACACCATGGACTACGCCATCGCCTTCGCCGAAACCGGCCATTTGTGCATGGCGACGCTGCACGCCAATTCGTCG
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGGCGCGCGCAACTGTTGATGGATCTGTCGCTCAACCT
CAAGGCCTTCGTGTCGCAGCGGCTGGTGCCGCATCGCTCGGGCAAGGGCCGCGTCGCTGCGGTCGAGGTGATGCTCAATT
CGCCGCTGATTTCCGATCTGATCTTCAAGGGCGAGGTCCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGAGCCTGTTCGACCTGTACGAGGCGGGCAAGATCAGTTACGAGGATGCGCTGCGCAACGCCGA
TTCGGTGAACGATTTGCGGCTGCAGATCAAACTGCAGGGTTCGGAATCGAAGCAGCGCGACGTGCTCTCCGGGATCGATC
ATCTCGACATTGTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(19-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.842

93.651

0.598
  pilU Acinetobacter baylyi ADP1

59.664

94.444

0.563
  pilU Vibrio cholerae strain A1552

55.425

90.212

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Pseudomonas aeruginosa PAK

42.433

89.153

0.378
  pilT Acinetobacter baylyi ADP1

44.237

84.921

0.376
  pilT Acinetobacter baumannii D1279779

42.342

88.095

0.373
  pilT Acinetobacter nosocomialis M2

42.342

88.095

0.373
  pilT Acinetobacter baumannii strain A118

42.342

88.095

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.566

87.831

0.365
  pilT Vibrio cholerae strain A1552

41.566

87.831

0.365

Multiple sequence alignment