Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FLM48_RS05960 Genome accession   NZ_CP041329
Coordinates   1349653..1350690 (+) Length   345 a.a.
NCBI ID   WP_028772617.1    Uniprot ID   -
Organism   Shewanella sp. Scap07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1344653..1355690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM48_RS05925 (FLM48_05990) hemW 1344736..1345878 (-) 1143 WP_179024018.1 radical SAM family heme chaperone HemW -
  FLM48_RS05930 (FLM48_05995) rdgB 1345878..1346480 (-) 603 WP_179024019.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FLM48_RS05935 (FLM48_06000) - 1346584..1347018 (-) 435 WP_179024020.1 DUF4426 domain-containing protein -
  FLM48_RS05940 (FLM48_06005) yggU 1347114..1347407 (-) 294 WP_179024021.1 DUF167 family protein YggU -
  FLM48_RS05945 (FLM48_06010) - 1347407..1347955 (-) 549 WP_179024022.1 YggT family protein -
  FLM48_RS05950 (FLM48_06015) proC 1347986..1348804 (-) 819 WP_028772615.1 pyrroline-5-carboxylate reductase -
  FLM48_RS05955 (FLM48_06020) - 1348922..1349617 (-) 696 WP_179024023.1 YggS family pyridoxal phosphate-dependent enzyme -
  FLM48_RS05960 (FLM48_06025) pilT 1349653..1350690 (+) 1038 WP_028772617.1 type IV pilus twitching motility protein PilT Machinery gene
  FLM48_RS05965 (FLM48_06030) pilU 1350704..1351816 (+) 1113 WP_028772618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FLM48_RS05970 (FLM48_06035) - 1351916..1353046 (+) 1131 WP_256871063.1 hypothetical protein -
  FLM48_RS05975 (FLM48_06040) - 1353137..1353679 (+) 543 WP_179024024.1 glutathione peroxidase -
  FLM48_RS05980 (FLM48_06045) hemH 1353709..1354716 (+) 1008 WP_179024025.1 ferrochelatase -
  FLM48_RS05985 (FLM48_06050) ruvX 1354777..1355196 (-) 420 WP_179024026.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38375.88 Da        Isoelectric Point: 6.7841

>NTDB_id=371219 FLM48_RS05960 WP_028772617.1 1349653..1350690(+) (pilT) [Shewanella sp. Scap07]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAYNQSRGAAAVFRTIPSDILSLEQLGAPEIFKKISSYPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQITREDAMQKSANKQTTF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=371219 FLM48_RS05960 WP_028772617.1 1349653..1350690(+) (pilT) [Shewanella sp. Scap07]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAACGCTTCAGATCTACACCTTAGTGCTGGCGTTTCGCCAAT
GATACGTGTTGATGGTGAAGTCAGAAAGATTAATCTGCCTGCTTTAGATCATCAAGGCGTACATAGCTTAGTCTATGACA
TTATGAATGATAAGCAGCGTAAAGATTATGAAGAACATTTAGAGATAGATTTTTCTTTTGAAGTGCCTAATTTAGCACGA
TTTCGTGTCAATGCTTATAATCAGTCTCGCGGCGCTGCAGCCGTTTTTCGTACCATTCCCAGTGATATTCTTAGCTTAGA
GCAATTAGGTGCTCCGGAAATTTTTAAGAAAATCTCTAGTTACCCGCGTGGTTTAGTACTGGTTACCGGGCCTACTGGTT
CTGGTAAAAGTACCACTTTGGCGGCGATGATTGATTACGTGAATGAAAATCGCCATGACCACATTCTGACTATTGAAGAC
CCGATAGAATTTGTACACCAAAACAAGCAATGTTTGATTAACCAACGTGAGGTTCATAAGCATACCCATAGCTTCAATGC
CGCACTAAGAAGCGCATTACGTGAAGATCCCGATGTTATTCTAGTGGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CGATGACCGCAGCGGAGACCGGTCATTTAGTATTCGGCACCTTGCATACCACCTCAGCGGCAAAAACCATTGACCGTGTG
GTGGATGTTTTCCCTGAAGGTGAAAAAGGCATGGTGCGGACCATGCTCTCTGAGTCATTGCAGGCGGTGATTTCGCAAAC
CCTGATTAAGAAAGTCGGCGGTGGTCGAGTGGCGGCTCATGAAATTATGATGGGTACCCCCGCGATTCGAAATCTAATCC
GTGAAGATAAAGTTGCGCAGATGTATTCGGCGATTCAGACCGGTATGGCTCATGGCATGCAAACACTGGATCAATGTCTG
CAAAATCTAGTCAATCGTGGCCAAATCACCCGCGAAGATGCCATGCAGAAAAGCGCGAATAAGCAAACGACATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

78.107

97.971

0.765
  pilT Pseudomonas stutzeri DSM 10701

77.515

97.971

0.759
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

74.487

98.841

0.736
  pilT Legionella pneumophila strain ERS1305867

74.487

98.841

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.98

99.42

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.667

97.391

0.406
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Acinetobacter baylyi ADP1

39.349

97.971

0.386

Multiple sequence alignment