Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FLM48_RS05965 Genome accession   NZ_CP041329
Coordinates   1350704..1351816 (+) Length   370 a.a.
NCBI ID   WP_028772618.1    Uniprot ID   -
Organism   Shewanella sp. Scap07     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1345704..1356816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM48_RS05930 (FLM48_05995) rdgB 1345878..1346480 (-) 603 WP_179024019.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FLM48_RS05935 (FLM48_06000) - 1346584..1347018 (-) 435 WP_179024020.1 DUF4426 domain-containing protein -
  FLM48_RS05940 (FLM48_06005) yggU 1347114..1347407 (-) 294 WP_179024021.1 DUF167 family protein YggU -
  FLM48_RS05945 (FLM48_06010) - 1347407..1347955 (-) 549 WP_179024022.1 YggT family protein -
  FLM48_RS05950 (FLM48_06015) proC 1347986..1348804 (-) 819 WP_028772615.1 pyrroline-5-carboxylate reductase -
  FLM48_RS05955 (FLM48_06020) - 1348922..1349617 (-) 696 WP_179024023.1 YggS family pyridoxal phosphate-dependent enzyme -
  FLM48_RS05960 (FLM48_06025) pilT 1349653..1350690 (+) 1038 WP_028772617.1 type IV pilus twitching motility protein PilT Machinery gene
  FLM48_RS05965 (FLM48_06030) pilU 1350704..1351816 (+) 1113 WP_028772618.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FLM48_RS05970 (FLM48_06035) - 1351916..1353046 (+) 1131 WP_256871063.1 hypothetical protein -
  FLM48_RS05975 (FLM48_06040) - 1353137..1353679 (+) 543 WP_179024024.1 glutathione peroxidase -
  FLM48_RS05980 (FLM48_06045) hemH 1353709..1354716 (+) 1008 WP_179024025.1 ferrochelatase -
  FLM48_RS05985 (FLM48_06050) ruvX 1354777..1355196 (-) 420 WP_179024026.1 Holliday junction resolvase RuvX -
  FLM48_RS05990 (FLM48_06055) - 1355249..1355809 (-) 561 WP_028772623.1 YqgE/AlgH family protein -
  FLM48_RS05995 (FLM48_06060) yciH 1355829..1356158 (-) 330 WP_179024027.1 stress response translation initiation inhibitor YciH -
  FLM48_RS06000 (FLM48_06065) - 1356308..1356622 (-) 315 WP_028772625.1 hypothetical protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41046.01 Da        Isoelectric Point: 5.5999

>NTDB_id=371220 FLM48_RS05965 WP_028772618.1 1350704..1351816(+) (pilU) [Shewanella sp. Scap07]
MDVLPFLKVMIERKASDLFITAGFPPSAKIDGELRPLAENPFSPEQSLAFVESLMTDVQKQEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETQIPLVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNSNARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPENKHQQLLFDLSLNLRGIVAQQLIPKSDGSGRRAAIEVLINTPRVASLIAKNELHVLKETMAKSNEQGMQTFDQA
LLQLYSEGEISYADALHHADSPNDLRLMIKLQNSDSPSSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=371220 FLM48_RS05965 WP_028772618.1 1350704..1351816(+) (pilU) [Shewanella sp. Scap07]
ATGGATGTCCTCCCATTTTTAAAGGTAATGATTGAGCGCAAAGCGTCTGACTTATTCATTACTGCGGGCTTTCCACCTAG
TGCAAAAATTGATGGTGAACTAAGACCGTTAGCGGAAAACCCATTTTCACCTGAGCAATCGCTAGCGTTTGTTGAGTCGC
TAATGACAGACGTGCAAAAACAGGAGTTTCATGAAAGCCGTGAATGTAACTTCGCTTTTGCTGCGAAAGATTTAGGGCGT
TTTCGTGTTAGTGCATTTTGGCAACGAGAGTCGCCAGGGTGTGTGATGCGTCGTATCGAAACCCAAATTCCTTTGGTAGA
CGACCTCAAACTACCGCCTATTTTGAAAGACTTGGTGATGAGTAAGCGTGGTCTGATTATTATGGTTGGTGGTACCGGTA
CGGGTAAGTCAACCTCGCTGGCTGCACTGGTTGGTTACCGCAACTCTAATGCCCGAGGGCACATCTTAACCATTGAGGAT
CCGGTTGAATTTGTCCACGACCATCGCAAAAGTATTATTACTCAGCGTGAGGTCGGCATCGATACTGACTCATTTGATGC
CGCGCTTAAGAGCTCTTTGCGCCAAGCGCCCGATGTGATTTTGATTGGTGAGATCCGTACCCAAGAAACCATGGAGTTTG
CGTTATCGTTTGCTGAAACCGGGCATTTATGTATGGCAACCTTACACGCCAACAACGCCAACCAAGCGCTGGACCGCATC
ATGCATTTAGTGCCAGAGAATAAGCATCAGCAATTGTTATTTGATTTATCTTTGAACTTGCGCGGCATTGTTGCTCAGCA
GCTCATTCCCAAGAGTGATGGCAGCGGCAGGCGTGCAGCCATTGAAGTGCTGATTAACACCCCGAGGGTTGCCAGCTTAA
TTGCCAAAAATGAGCTGCACGTGCTGAAAGAAACCATGGCTAAATCAAATGAGCAAGGAATGCAGACATTTGACCAAGCT
TTACTACAGTTATATAGCGAAGGTGAAATCTCCTACGCAGATGCACTGCATCACGCTGACTCGCCTAATGACTTGCGTCT
GATGATTAAGCTGCAAAATTCAGACTCACCGAGTTCAGGTTTTATGGAAGGGGTAACCCTAGACCTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilU Vibrio cholerae strain A1552

57.838

100

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.243

0.386
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.515

89.189

0.37
  pilT Vibrio cholerae strain A1552

41.515

89.189

0.37
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362

Multiple sequence alignment