Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ECL_RS21340 Genome accession   NC_014121
Coordinates   4390474..4391454 (-) Length   326 a.a.
NCBI ID   WP_044157573.1    Uniprot ID   -
Organism   Enterobacter cloacae subsp. cloacae ATCC 13047     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4385474..4396454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECL_RS21310 (ECL_04273) - 4386350..4386847 (+) 498 WP_028027997.1 SprT family zinc-dependent metalloprotease -
  ECL_RS21315 (ECL_04274) endA 4386942..4387649 (+) 708 WP_013098661.1 deoxyribonuclease I -
  ECL_RS21320 (ECL_04275) rsmE 4387702..4388433 (+) 732 WP_013098662.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ECL_RS21325 (ECL_04276) gshB 4388453..4389400 (+) 948 WP_013098663.1 glutathione synthase -
  ECL_RS21330 (ECL_04277) - 4389487..4390047 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  ECL_RS21335 (ECL_04278) ruvX 4390047..4390463 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  ECL_RS21340 (ECL_04279) pilT 4390474..4391454 (-) 981 WP_044157573.1 type IV pilus twitching motility protein PilT Machinery gene
  ECL_RS21345 (ECL_04280) - 4391472..4392173 (+) 702 WP_013098666.1 YggS family pyridoxal phosphate-dependent enzyme -
  ECL_RS21350 (ECL_04281) - 4392195..4392761 (+) 567 WP_013098667.1 YggT family protein -
  ECL_RS21355 (ECL_04282) yggU 4392758..4393045 (+) 288 WP_013098668.1 DUF167 family protein YggU -
  ECL_RS21360 (ECL_04283) - 4393058..4393651 (+) 594 WP_013098669.1 XTP/dITP diphosphatase -
  ECL_RS21365 (ECL_04284) hemW 4393644..4394792 (+) 1149 WP_013098670.1 radical SAM family heme chaperone HemW -
  ECL_RS21370 - 4394828..4394947 (+) 120 Protein_4220 DUF559 domain-containing protein -
  ECL_RS21375 (ECL_04285) - 4395015..4395731 (-) 717 WP_013098671.1 DUF2884 domain-containing protein -
  ECL_RS21380 (ECL_04286) - 4395788..4396114 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35735.79 Da        Isoelectric Point: 6.4999

>NTDB_id=37107 ECL_RS21340 WP_044157573.1 4390474..4391454(-) (pilT) [Enterobacter cloacae subsp. cloacae ATCC 13047]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGRGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLIDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=37107 ECL_RS21340 WP_044157573.1 4390474..4391454(-) (pilT) [Enterobacter cloacae subsp. cloacae ATCC 13047]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACCCTTACGGGAAACCAGCGTCTGCGCGCA
AGTGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCTTTGGG
TGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGTGGGCTGATACTGGTGACGGGGGCAACCGGCCGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGTGAACGTTGCCTGATTCAGCAGCGAGAGATCGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCGGTGCCTCTCAGGCGATTGAACGGCTGATCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365
  pilB Haemophilus influenzae 86-028NP

33.427

100

0.365

Multiple sequence alignment