Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FKV23_RS03825 Genome accession   NZ_CP041242
Coordinates   899743..900780 (+) Length   345 a.a.
NCBI ID   WP_141622664.1    Uniprot ID   A0A514BPK8
Organism   Marilutibacter alkalisoli strain SJ-36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 894743..905780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKV23_RS03790 (FKV23_03790) - 894874..895077 (+) 204 WP_141622660.1 dodecin family protein -
  FKV23_RS03795 (FKV23_03795) - 895064..895942 (-) 879 WP_244244086.1 kinase -
  FKV23_RS03800 (FKV23_03800) pdxH 895960..896577 (+) 618 WP_141622661.1 pyridoxamine 5'-phosphate oxidase -
  FKV23_RS03805 (FKV23_03805) - 896550..897371 (+) 822 WP_141625023.1 cobalamin-binding protein -
  FKV23_RS03810 (FKV23_03810) - 897422..897907 (-) 486 WP_141622662.1 DUF4426 domain-containing protein -
  FKV23_RS03815 (FKV23_03815) proC 897910..898761 (-) 852 WP_141622663.1 pyrroline-5-carboxylate reductase -
  FKV23_RS03820 (FKV23_03820) - 898835..899551 (-) 717 WP_244244087.1 YggS family pyridoxal phosphate-dependent enzyme -
  FKV23_RS03825 (FKV23_03825) pilT 899743..900780 (+) 1038 WP_141622664.1 type IV pilus twitching motility protein PilT Machinery gene
  FKV23_RS03830 (FKV23_03830) pilU 900933..902063 (+) 1131 WP_141622665.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FKV23_RS03835 (FKV23_03835) - 902292..902990 (+) 699 WP_141622666.1 TraX family protein -
  FKV23_RS03840 (FKV23_03840) - 903176..903658 (+) 483 WP_141622667.1 peptidylprolyl isomerase -
  FKV23_RS03845 (FKV23_03845) - 903790..904362 (+) 573 WP_141622668.1 YqgE/AlgH family protein -
  FKV23_RS03850 (FKV23_03850) ruvX 904388..904867 (+) 480 WP_141622669.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38537.22 Da        Isoelectric Point: 6.9398

>NTDB_id=370765 FKV23_RS03825 WP_141622664.1 899743..900780(+) (pilT) [Marilutibacter alkalisoli strain SJ-36]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGTPRIFKDLIDQPQGLILVTGPTGSGKSTTLAAMLDHVNKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQQHGMMTLDQHL
QDLVKRGLVARQQARQYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=370765 FKV23_RS03825 WP_141622664.1 899743..900780(+) (pilT) [Marilutibacter alkalisoli strain SJ-36]
ATGGATATCGCCGAACTACTGGCGTTCTCGGTCAAGAACAAGGCCTCGGACCTGCATCTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCGCTCGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAGGTGCTGACCCTGGA
GGACTTGGGGACTCCACGCATCTTCAAGGACCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCCACCACGCTGGCGGCGATGCTCGACCACGTCAACAAGAACGAGTACGCACACATCCTCTCGGTCGAGGAC
CCGATCGAATTCGTCCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCATTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAGCTGCGCGACCTTGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCAGCCAAGACCGTCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTCCGCTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACCGCGGCGTGGGAGATCATGGTCGGCATCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGCACGGCATGATGACCCTCGACCAGCACCTG
CAGGACCTGGTCAAGCGCGGCCTGGTGGCCCGCCAGCAGGCCCGCCAGTACGCCAAGGACAAGCGGCTGTTCGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514BPK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.814

99.71

0.806
  pilT Acinetobacter baumannii strain A118

80.814

99.71

0.806
  pilT Acinetobacter nosocomialis M2

80.814

99.71

0.806
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.149

97.101

0.681
  pilT Vibrio cholerae strain A1552

70.149

97.101

0.681
  pilT Neisseria meningitidis 8013

65.497

99.13

0.649
  pilT Neisseria gonorrhoeae MS11

65.205

99.13

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.484
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Vibrio cholerae strain A1552

39.701

97.101

0.386
  pilU Acinetobacter baylyi ADP1

38.824

98.551

0.383

Multiple sequence alignment