Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FKV23_RS03830 Genome accession   NZ_CP041242
Coordinates   900933..902063 (+) Length   376 a.a.
NCBI ID   WP_141622665.1    Uniprot ID   A0A514BPM2
Organism   Marilutibacter alkalisoli strain SJ-36     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895933..907063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKV23_RS03800 (FKV23_03800) pdxH 895960..896577 (+) 618 WP_141622661.1 pyridoxamine 5'-phosphate oxidase -
  FKV23_RS03805 (FKV23_03805) - 896550..897371 (+) 822 WP_141625023.1 cobalamin-binding protein -
  FKV23_RS03810 (FKV23_03810) - 897422..897907 (-) 486 WP_141622662.1 DUF4426 domain-containing protein -
  FKV23_RS03815 (FKV23_03815) proC 897910..898761 (-) 852 WP_141622663.1 pyrroline-5-carboxylate reductase -
  FKV23_RS03820 (FKV23_03820) - 898835..899551 (-) 717 WP_244244087.1 YggS family pyridoxal phosphate-dependent enzyme -
  FKV23_RS03825 (FKV23_03825) pilT 899743..900780 (+) 1038 WP_141622664.1 type IV pilus twitching motility protein PilT Machinery gene
  FKV23_RS03830 (FKV23_03830) pilU 900933..902063 (+) 1131 WP_141622665.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FKV23_RS03835 (FKV23_03835) - 902292..902990 (+) 699 WP_141622666.1 TraX family protein -
  FKV23_RS03840 (FKV23_03840) - 903176..903658 (+) 483 WP_141622667.1 peptidylprolyl isomerase -
  FKV23_RS03845 (FKV23_03845) - 903790..904362 (+) 573 WP_141622668.1 YqgE/AlgH family protein -
  FKV23_RS03850 (FKV23_03850) ruvX 904388..904867 (+) 480 WP_141622669.1 Holliday junction resolvase RuvX -
  FKV23_RS03855 (FKV23_03855) - 904885..905847 (+) 963 WP_141622670.1 aspartate carbamoyltransferase catalytic subunit -
  FKV23_RS03860 (FKV23_03860) - 905979..906341 (-) 363 WP_141622671.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41862.17 Da        Isoelectric Point: 6.6612

>NTDB_id=370766 FKV23_RS03830 WP_141622665.1 900933..902063(+) (pilU) [Marilutibacter alkalisoli strain SJ-36]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNALTSQQSRDLVLNVMNPQQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELSLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLVPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDAQTLAQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=370766 FKV23_RS03830 WP_141622665.1 900933..902063(+) (pilU) [Marilutibacter alkalisoli strain SJ-36]
ATGAGCACGATCGACTTCACCTCCTTCCTCAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTGCACGGCAAGATCTCGCCGATCACCCAGAACGCACTGACCTCGCAGCAGAGCCGCGACCTCG
TGCTCAACGTGATGAACCCGCAGCAGCGCGAGGAGTTCGAGAAGACCCACGAATGCAACTTCGCGATCGGCGTGGCCGGT
GTCGGCCGCTTCCGCGTCAGTTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
GACCGTCGAGGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGTG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACTCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCGGCCAGTTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGGTACCGACCCCCGACGGCAAGGCCCGCCGCGTCGCGATGGAAATCCTGCTCGGCACCCCGCTGGTCC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAATCCGTGCAGCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAAGACGCCCTGCGCTACGCCGACTCCCAGAACGA
AGTCCGCCTGCGCATCAAACTCGCCCAGGGCGGCGACGCGCAGACACTGGCGCAGGGGTTGGATGGGGTGGAAGTCGCCG
AGGTGCGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514BPM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

97.606

0.662
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Acinetobacter nosocomialis M2

40.698

91.489

0.372
  pilT Acinetobacter baumannii D1279779

40.698

91.489

0.372
  pilT Acinetobacter baumannii strain A118

40.698

91.489

0.372

Multiple sequence alignment