Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FJQ89_RS22305 Genome accession   NZ_CP041185
Coordinates   5078486..5079529 (-) Length   347 a.a.
NCBI ID   WP_141171744.1    Uniprot ID   A0A4Y6RJW2
Organism   Janthinobacterium tructae strain SNU WT1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5073486..5084529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJQ89_RS22290 (FJQ89_22290) - 5074560..5076671 (-) 2112 WP_141171742.1 EAL domain-containing protein -
  FJQ89_RS22295 (FJQ89_22295) - 5076855..5077340 (-) 486 WP_141171743.1 glutathione peroxidase -
  FJQ89_RS22300 (FJQ89_22300) pilU 5077342..5078481 (-) 1140 WP_205704519.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FJQ89_RS22305 (FJQ89_22305) pilT 5078486..5079529 (-) 1044 WP_141171744.1 type IV pilus twitching motility protein PilT Machinery gene
  FJQ89_RS22310 (FJQ89_22310) - 5079600..5080304 (+) 705 WP_141171745.1 YggS family pyridoxal phosphate-dependent enzyme -
  FJQ89_RS22315 (FJQ89_22315) proC 5080323..5081153 (+) 831 WP_141171746.1 pyrroline-5-carboxylate reductase -
  FJQ89_RS22320 (FJQ89_22320) - 5081178..5081894 (-) 717 WP_141171747.1 anti-sigma factor -
  FJQ89_RS22325 (FJQ89_22325) - 5081891..5082448 (-) 558 WP_141171748.1 sigma-70 family RNA polymerase sigma factor -
  FJQ89_RS22330 (FJQ89_22330) - 5082555..5083112 (-) 558 WP_141171749.1 DUF3455 domain-containing protein -
  FJQ89_RS22335 (FJQ89_22335) - 5083202..5083549 (-) 348 WP_141171750.1 hypothetical protein -
  FJQ89_RS22340 (FJQ89_22340) - 5083546..5083932 (-) 387 WP_141171751.1 phage holin family protein -
  FJQ89_RS22345 (FJQ89_22345) - 5083980..5084336 (-) 357 WP_046683402.1 DUF883 family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38054.55 Da        Isoelectric Point: 6.6919

>NTDB_id=370282 FJQ89_RS22305 WP_141171744.1 5078486..5079529(-) (pilT) [Janthinobacterium tructae strain SNU WT1]
MDISELLAFSVSNKASDLHLSSGLPPMIRVHGDVRRLNVAPLEHKEVHSMIYDIMNDSQRKAYEEALECDFSFEIPGLAR
FRVNAYNQERGASAVLRTIPSKVLTLEELNAPRIFGELAMRPRGLVLVTGPTGSGKSTTLAAMVNHVNERLNHHILTIED
PIEFVHEPKKCLINQREVGSHTHSFSNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKSIDRI
IDVFPAEEKEMVRAMLSESLQAVISQNLLKTKDGAGRVAAHEIMLATPAVRNLIREAKVAQMYSAIQTGSNVGMQTLDQC
LSDLVRRGTISAEAARSAAKSPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=370282 FJQ89_RS22305 WP_141171744.1 5078486..5079529(-) (pilT) [Janthinobacterium tructae strain SNU WT1]
ATGGACATCTCCGAATTACTCGCTTTCTCCGTCAGCAACAAGGCTTCCGACCTGCACCTGTCTTCCGGCCTGCCGCCGAT
GATACGCGTCCATGGCGACGTGCGCCGCCTGAACGTGGCGCCGCTCGAGCACAAGGAAGTGCACAGTATGATCTACGACA
TCATGAACGACAGCCAGCGCAAGGCCTATGAAGAAGCGCTGGAATGCGATTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTACAACCAGGAACGGGGCGCTTCGGCCGTGCTGCGCACGATTCCCTCAAAAGTGCTGACACTGGA
AGAACTCAACGCCCCGCGCATCTTCGGCGAGCTGGCCATGCGTCCGCGCGGCCTGGTGCTCGTCACGGGTCCGACGGGCT
CCGGCAAGTCGACCACCCTGGCGGCCATGGTGAACCACGTCAACGAACGCCTGAACCACCATATTCTGACCATCGAAGAT
CCGATCGAATTCGTGCATGAGCCAAAAAAATGCCTGATCAACCAGCGCGAGGTGGGCTCGCACACGCATTCGTTCAGCAA
CGCGCTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGATTTTAGTGGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACGGCAGCCGAAACGGGCCACCTGGTGTTCGGCACCCTGCATACCTCGTCGGCGGCCAAGTCGATCGACCGCATC
ATCGACGTCTTTCCTGCAGAGGAAAAGGAAATGGTGCGCGCCATGCTGTCCGAATCCTTGCAAGCCGTCATTTCGCAGAA
TTTGCTGAAAACCAAGGACGGCGCGGGCCGCGTGGCCGCGCATGAAATCATGCTGGCCACGCCCGCCGTGCGCAACCTGA
TCCGCGAAGCGAAAGTGGCGCAGATGTATTCGGCCATCCAGACGGGCAGCAACGTGGGCATGCAGACCCTGGACCAGTGC
CTCTCCGACCTGGTGCGGCGCGGCACGATCTCGGCCGAAGCGGCCCGCTCGGCCGCCAAATCGCCTGAAAACTTCCCCGG
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y6RJW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.072

99.424

0.746
  pilT Acinetobacter baumannii D1279779

75.072

99.424

0.746
  pilT Acinetobacter baumannii strain A118

75.072

99.424

0.746
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

71.091

97.695

0.695
  pilT Legionella pneumophila strain ERS1305867

71.091

97.695

0.695
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Neisseria meningitidis 8013

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

97.118

0.683
  pilT Vibrio cholerae strain A1552

70.326

97.118

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.424

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.159

99.424

0.409
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Vibrio cholerae strain A1552

40.87

99.424

0.406

Multiple sequence alignment