Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FGA12_RS13610 Genome accession   NZ_CP041153
Coordinates   3074531..3075568 (-) Length   345 a.a.
NCBI ID   WP_033539855.1    Uniprot ID   A0AAC9TZ45
Organism   Shewanella marisflavi strain ECSMB14101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3069531..3080568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGA12_RS13595 (FGA12_13595) - 3070631..3071644 (+) 1014 WP_140946979.1 IS110 family transposase -
  FGA12_RS13600 (FGA12_13600) hemH 3072182..3073189 (-) 1008 WP_033539857.1 ferrochelatase -
  FGA12_RS13605 (FGA12_13605) pilU 3073406..3074518 (-) 1113 WP_033539856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FGA12_RS13610 (FGA12_13610) pilT 3074531..3075568 (-) 1038 WP_033539855.1 type IV pilus twitching motility protein PilT Machinery gene
  FGA12_RS13615 (FGA12_13615) - 3075604..3076305 (+) 702 WP_033539854.1 YggS family pyridoxal phosphate-dependent enzyme -
  FGA12_RS13620 (FGA12_13620) proC 3076372..3077190 (+) 819 WP_033539853.1 pyrroline-5-carboxylate reductase -
  FGA12_RS13625 (FGA12_13625) - 3077248..3077796 (+) 549 WP_033539852.1 YggT family protein -
  FGA12_RS13630 (FGA12_13630) yggU 3077797..3078087 (+) 291 WP_033539851.1 DUF167 family protein YggU -
  FGA12_RS13635 (FGA12_13635) - 3078174..3078608 (+) 435 WP_033539850.1 DUF4426 domain-containing protein -
  FGA12_RS13640 (FGA12_13640) rdgB 3078747..3079346 (+) 600 WP_033539866.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FGA12_RS13645 (FGA12_13645) hemW 3079346..3080485 (+) 1140 WP_033539849.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38273.83 Da        Isoelectric Point: 6.9095

>NTDB_id=369954 FGA12_RS13610 WP_033539855.1 3074531..3075568(-) (pilT) [Shewanella marisflavi strain ECSMB14101]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEKLGAPDIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMSKSSNKNASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=369954 FGA12_RS13610 WP_033539855.1 3074531..3075568(-) (pilT) [Shewanella marisflavi strain ECSMB14101]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAACGCGTCGGATCTACACCTTTCAGCGGGAGTATCTCCCAT
GATACGTGTTGATGGCGAGGTCAGAAAGATTAATCTGCCGGCCCTGGATCATCAAGGCGTACATAGCCTCGTTTATGACA
TCATGAACGACAAACAGCGTAAAGATTATGAAGAGCACTTAGAGATAGATTTCTCGTTCGAAGTGCCCAATTTAGCTCGC
TTCCGTGTGAACGCCTTTAACCAATCGCGCGGCGCCGCGGCGGTGTTTCGTACTATTCCCAGCGAGATCTTAAGCCTTGA
AAAACTCGGCGCCCCCGATATTTTCAAAAAGATTGCCAGCTTCCCTCGTGGCCTGGTGCTGGTAACCGGCCCCACAGGTT
CGGGTAAGAGTACCACCCTGGCGGGCATGATAGATTATGTGAACGAAAACCGTCACGACCATATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCACCAGAATAAGCAATGTCTGATCAACCAGAGGGAAGTGCACAGACACACCCACAGCTTCAACGC
GGCACTGCGTAGCGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGTGACCTTGAAACCATTCGTCTGG
CCATGACGGCGGCGGAAACCGGTCACTTAGTATTCGGTACACTGCACACCACCTCAGCGGCGAAAACTATTGACCGTGTG
GTCGACGTTTTCCCTGAAGGTGAAAAGAGTATGGTACGTACCATGTTGTCTGAATCACTGCAGGCGGTTATCTCGCAAAC
CCTGATCAAAAAGGTAGGCGGCGGCCGTGTGGCAGCTCACGAGATCATGATGGGTACACCGGCTATCCGTAACCTGATCC
GTGAAGACAAGGTGGCCCAGATGTACTCGGCCATTCAAACGGGTATGGCTCACGGGATGCAGACTCTGGATCAATGCTTG
CAGAATCTGGTCAATCGCGGCCTGATCACCCGTGAAGATGCAATGTCTAAGAGTTCTAATAAAAACGCTAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.138

96.812

0.786
  pilT Acinetobacter baumannii D1279779

81.138

96.812

0.786
  pilT Acinetobacter baumannii strain A118

81.138

96.812

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.107

97.971

0.765
  pilT Pseudomonas aeruginosa PAK

78.107

97.971

0.765
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

75.146

99.13

0.745
  pilT Legionella pneumophila strain ERS1305867

75.146

99.13

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.691

99.42

0.414
  pilU Vibrio cholerae strain A1552

40.233

99.42

0.4
  pilU Acinetobacter baylyi ADP1

39.535

99.71

0.394

Multiple sequence alignment