Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FGA12_RS13605 Genome accession   NZ_CP041153
Coordinates   3073406..3074518 (-) Length   370 a.a.
NCBI ID   WP_033539856.1    Uniprot ID   A0AAC9U1X7
Organism   Shewanella marisflavi strain ECSMB14101     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3068406..3079518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGA12_RS13590 (FGA12_13590) - 3069492..3070037 (-) 546 WP_200884297.1 tetratricopeptide repeat protein -
  FGA12_RS13595 (FGA12_13595) - 3070631..3071644 (+) 1014 WP_140946979.1 IS110 family transposase -
  FGA12_RS13600 (FGA12_13600) hemH 3072182..3073189 (-) 1008 WP_033539857.1 ferrochelatase -
  FGA12_RS13605 (FGA12_13605) pilU 3073406..3074518 (-) 1113 WP_033539856.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FGA12_RS13610 (FGA12_13610) pilT 3074531..3075568 (-) 1038 WP_033539855.1 type IV pilus twitching motility protein PilT Machinery gene
  FGA12_RS13615 (FGA12_13615) - 3075604..3076305 (+) 702 WP_033539854.1 YggS family pyridoxal phosphate-dependent enzyme -
  FGA12_RS13620 (FGA12_13620) proC 3076372..3077190 (+) 819 WP_033539853.1 pyrroline-5-carboxylate reductase -
  FGA12_RS13625 (FGA12_13625) - 3077248..3077796 (+) 549 WP_033539852.1 YggT family protein -
  FGA12_RS13630 (FGA12_13630) yggU 3077797..3078087 (+) 291 WP_033539851.1 DUF167 family protein YggU -
  FGA12_RS13635 (FGA12_13635) - 3078174..3078608 (+) 435 WP_033539850.1 DUF4426 domain-containing protein -
  FGA12_RS13640 (FGA12_13640) rdgB 3078747..3079346 (+) 600 WP_033539866.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41226.27 Da        Isoelectric Point: 6.1913

>NTDB_id=369953 FGA12_RS13605 WP_033539856.1 3073406..3074518(-) (pilU) [Shewanella marisflavi strain ECSMB14101]
MEVRPFLKVMVERKASDLFITAGFPPSAKVDGEVRPLAENPFTPAQSLEFVEALMTEAQRKEFHETRECNFAYGEKDLGR
FRVSAFWQRESPGCVMRRIETKIPLVEDLHLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKSDGTGRRAAIEVLINTPRVSSIIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVEGEISYSDALHHADSPNDLRLMIKLQSSESASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=369953 FGA12_RS13605 WP_033539856.1 3073406..3074518(-) (pilU) [Shewanella marisflavi strain ECSMB14101]
ATGGAAGTTCGTCCATTTTTAAAGGTGATGGTAGAGCGTAAGGCATCGGATCTATTCATCACGGCTGGTTTTCCTCCCAG
CGCCAAGGTTGATGGTGAGGTGCGACCCCTTGCCGAAAATCCCTTTACGCCGGCTCAGTCACTGGAGTTTGTCGAAGCCT
TGATGACAGAGGCTCAGCGCAAAGAGTTTCACGAGACCCGCGAATGTAACTTCGCTTATGGCGAGAAGGATCTGGGGCGT
TTCCGTGTTAGCGCCTTCTGGCAGCGAGAGTCGCCAGGTTGCGTAATGCGTCGCATCGAGACCAAGATCCCGCTAGTCGA
AGATCTTCATCTGCCGCCAATTTTGAAAGACTTAGTGATGAGTAAGCGCGGTCTTATCATCATGGTGGGTGGTACGGGTA
CGGGTAAGTCGACTTCGCTCGCGGCCCTGGTGGGCTATCGCAATGCCCATGCTCGCGGTCATATTCTGACCATCGAAGAT
CCGGTGGAATTTGTCCATGACCATCGTAAGAGCATCATCACTCAGCGAGAGGTAGGTATAGATACTGATTCTTTCGATGC
TGCGCTAAAGAGTTCTTTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGAACCCAGGAAACCATGGAGTTTG
CCCTGTCGTTCGCCGAAACCGGTCACCTCTGTATGGCAACCTTGCACGCCAACAACGCTAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCAGAGAGTAAGCATCAGCAGTTACTGTTCGATTTGTCGTTAAACCTGCGCGGCATTGTGGCGCAGCA
GTTGGTGCCAAAGAGTGACGGTACGGGTCGCCGTGCGGCCATCGAGGTATTGATTAACACCCCAAGGGTTTCTAGCATTA
TCCAGAAGAACGAGCTGCATCTGCTTAAGGAAACCATGGCGAAATCCAACGAGCAGGGGATGCAGACCTTTGACCAGGCG
CTACTCAAGCTCTATGTGGAAGGTGAGATCAGCTACTCGGATGCATTGCATCACGCCGACTCGCCAAACGATCTGCGCTT
GATGATTAAGCTGCAAAGCTCAGAGTCTGCAAGTTCAGGCTTTATGGAAGGTGTGACGCTGGATCTCGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.186

100

0.614
  pilU Acinetobacter baylyi ADP1

60.446

97.027

0.586
  pilU Vibrio cholerae strain A1552

57.838

100

0.578
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.444

97.297

0.384
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365

Multiple sequence alignment