Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIV34_RS15520 Genome accession   NZ_CP041046
Coordinates   3411013..3412050 (-) Length   345 a.a.
NCBI ID   WP_139984316.1    Uniprot ID   A0A4Y5Z5V0
Organism   Luteibacter pinisoli strain MAH-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3406013..3417050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV34_RS15500 (FIV34_15500) - 3406043..3406969 (+) 927 WP_139984307.1 aspartate carbamoyltransferase catalytic subunit -
  FIV34_RS15505 (FIV34_15505) - 3407046..3408794 (+) 1749 WP_139984310.1 hypothetical protein -
  FIV34_RS15510 (FIV34_15510) - 3409340..3409705 (+) 366 WP_139984312.1 BON domain-containing protein -
  FIV34_RS15515 (FIV34_15515) pilU 3409756..3410919 (-) 1164 WP_139984314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV34_RS15520 (FIV34_15520) pilT 3411013..3412050 (-) 1038 WP_139984316.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV34_RS15525 (FIV34_15525) proC 3412168..3412992 (+) 825 WP_139984318.1 pyrroline-5-carboxylate reductase -
  FIV34_RS15530 (FIV34_15530) - 3412989..3413564 (+) 576 WP_139984320.1 YggT family protein -
  FIV34_RS15535 (FIV34_15535) - 3413569..3415701 (-) 2133 WP_139984322.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38448.12 Da        Isoelectric Point: 6.8315

>NTDB_id=369168 FIV34_RS15520 WP_139984316.1 3411013..3412050(-) (pilT) [Luteibacter pinisoli strain MAH-14]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNHNRGAGGVFRTIPSEVLTLEDLGAPKIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHLLTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSAIQTGQQHGMQTLDQNL
SDLIKRGLITRQEGLMYAKNKEGFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=369168 FIV34_RS15520 WP_139984316.1 3411013..3412050(-) (pilT) [Luteibacter pinisoli strain MAH-14]
ATGGATATCGCCGAACTCCTTGCCTTCTCAGTGAAGAACAAGGCCTCCGACCTGCACCTTTCCGCAGGCCTTCCGCCCAT
GATCCGCGTGGACGGTGACGTTCGGCGCATCAACATCCCGGCGCTGGAACACAAGCAGGTCCATTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTATGAGGAATTCCTTGAAGTCGACTTCTCCTTCGAGATTCCCGGCCTGGCCCGC
TTCCGCGTCAACGCCTTCAACCACAACCGTGGCGCGGGCGGCGTGTTCCGTACCATTCCGTCCGAAGTGCTCACCCTGGA
AGACCTCGGCGCGCCGAAGATCTTCCGCGAGCTGATCGAGCAGCCGCAGGGGCTGATCCTGGTCACCGGCCCCACGGGTT
CGGGTAAGTCGACCACCCTGGCGGCCATGGTCGACCACATCAACAAGAACGAATACGGCCACCTGCTGACGGTGGAAGAC
CCCATCGAATTCGTCCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAAGTCCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGATCCCGACTACATCCTGGTGGGCGAGCTTCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGGCACCCTGCACACCTCGTCCGCCGCCAAGACCATCGACCGAATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTCTCCGAGTCGCTGCGTGCCGTTATCTCGCAGTC
CCTGCTGAAGAAGGTGGGCGGTGGCCGTATCGCGGCCCACGAGATCATGGTGGGCATCCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTGGCCCAGATGTACTCGGCCATCCAGACCGGCCAGCAGCACGGCATGCAGACCCTGGACCAGAACCTG
TCCGACCTGATCAAGCGGGGCCTCATCACCCGCCAGGAAGGCCTGATGTACGCCAAAAACAAGGAAGGCTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y5Z5V0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.772

96.522

0.693
  pilT Vibrio cholerae strain A1552

71.772

96.522

0.693
  pilT Neisseria meningitidis 8013

64.348

100

0.643
  pilT Neisseria gonorrhoeae MS11

64.058

100

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

39.017

100

0.391
  pilU Acinetobacter baylyi ADP1

38.617

100

0.388

Multiple sequence alignment