Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIV34_RS15515 Genome accession   NZ_CP041046
Coordinates   3409756..3410919 (-) Length   387 a.a.
NCBI ID   WP_139984314.1    Uniprot ID   A0A4Y5Z8P1
Organism   Luteibacter pinisoli strain MAH-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3404756..3415919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV34_RS15490 (FIV34_15490) - 3405020..3405586 (+) 567 WP_139984302.1 YqgE/AlgH family protein -
  FIV34_RS15495 (FIV34_15495) ruvX 3405590..3406009 (+) 420 WP_139984305.1 Holliday junction resolvase RuvX -
  FIV34_RS15500 (FIV34_15500) - 3406043..3406969 (+) 927 WP_139984307.1 aspartate carbamoyltransferase catalytic subunit -
  FIV34_RS15505 (FIV34_15505) - 3407046..3408794 (+) 1749 WP_139984310.1 hypothetical protein -
  FIV34_RS15510 (FIV34_15510) - 3409340..3409705 (+) 366 WP_139984312.1 BON domain-containing protein -
  FIV34_RS15515 (FIV34_15515) pilU 3409756..3410919 (-) 1164 WP_139984314.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV34_RS15520 (FIV34_15520) pilT 3411013..3412050 (-) 1038 WP_139984316.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV34_RS15525 (FIV34_15525) proC 3412168..3412992 (+) 825 WP_139984318.1 pyrroline-5-carboxylate reductase -
  FIV34_RS15530 (FIV34_15530) - 3412989..3413564 (+) 576 WP_139984320.1 YggT family protein -
  FIV34_RS15535 (FIV34_15535) - 3413569..3415701 (-) 2133 WP_139984322.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43215.44 Da        Isoelectric Point: 6.9602

>NTDB_id=369167 FIV34_RS15515 WP_139984314.1 3409756..3410919(-) (pilU) [Luteibacter pinisoli strain MAH-14]
MSEFDFTSFLKLMVHKQASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPTQREEFEKTHECQFAISASG
VGRFRVSCFYQRNCVGMVLRRIESKIPTPEELNLPPIIKQLAMTKRGIIIFVGGTGTGKSTSLASMVGYRNQNSTGHIIT
IEDPIEYVHRHEGCIITQRELGIDTDSWDNALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAL
DRILHFFPEDRRSQLLMDLSLNLKGIVAQQLIPTPDGKARRVAVEVLLGTPLAQDYIRQGDVHKLKELMRDSNQLGMRTF
DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLEGVEIDKANDKSTFGGMLSR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=369167 FIV34_RS15515 WP_139984314.1 3409756..3410919(-) (pilU) [Luteibacter pinisoli strain MAH-14]
ATGAGCGAGTTCGATTTCACCTCCTTCCTTAAGCTGATGGTGCACAAACAGGCGTCCGACCTGTTTATCACCGCCGGCGT
CGCGCCTTCCATGAAGGTGCAGGGCCGTATCGTGCCCATCACCCAGAGCCCCCTGTCGGCGCAGCAGTCGCGCGACATGG
TGCTCAACGTGATGACGCCCACCCAGCGCGAGGAATTTGAAAAGACCCACGAATGCCAGTTCGCGATCAGCGCGTCGGGC
GTGGGCCGTTTCCGCGTGTCGTGTTTCTACCAGCGCAACTGCGTTGGCATGGTCCTGCGCCGCATCGAATCGAAGATCCC
CACGCCGGAAGAGCTGAACCTGCCGCCGATCATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
GTACCGGTACGGGTAAGTCCACCTCGCTCGCGTCGATGGTCGGCTACCGCAACCAGAACTCCACCGGCCACATCATCACG
ATCGAAGACCCGATCGAATACGTGCACCGCCACGAGGGCTGCATCATCACCCAGCGCGAGCTGGGCATCGATACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTCATCATGATCGGCGAAGTGCGTACCCGCGAAACGA
TGGAACACGCCATCAACTTCTCGGAAACCGGCCACCTCTGCCTGTGCACCCTGCATGCGAACAACGCCAACCAGGCACTG
GACCGCATCCTGCACTTCTTCCCGGAAGACCGCCGCTCGCAGCTGCTGATGGATCTGTCGCTGAACCTGAAGGGCATCGT
CGCCCAGCAGCTGATCCCCACGCCCGATGGCAAGGCCCGTCGCGTTGCGGTCGAAGTTCTGCTCGGCACGCCGCTGGCCC
AGGATTACATCCGCCAGGGCGACGTACACAAGCTCAAGGAGCTGATGCGCGACTCCAACCAGCTGGGCATGCGCACGTTC
GACCAGAGCCTGGTGGAGCTGTACCACGCCGGCGAGATCTCGTACGAAGACGCCCTGCGCCACGCCGACAGCTCGAACGA
AGTGCGCCTGCGCATCAAGCTCGCGCAGGGCGGCGACGCACACACCCTGTCGCAGGGCCTGGAAGGCGTCGAGATCGACA
AGGCCAACGACAAGAGCACATTCGGCGGCATGCTTAGCCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y5Z8P1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.508

96.641

0.633
  pilU Acinetobacter baylyi ADP1

62.36

91.99

0.574
  pilU Vibrio cholerae strain A1552

51.081

95.607

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.2

96.899

0.38

Multiple sequence alignment