Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SVI_RS04910 Genome accession   NC_014012
Coordinates   1216418..1217455 (+) Length   345 a.a.
NCBI ID   WP_013050326.1    Uniprot ID   D4ZH66
Organism   Shewanella violacea DSS12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1211418..1222455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVI_RS04880 (SVI_1038) rdgB 1212457..1213059 (-) 603 WP_013050320.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  SVI_RS04885 (SVI_1039) - 1213210..1213644 (-) 435 WP_013050321.1 DUF4426 domain-containing protein -
  SVI_RS04890 (SVI_1040) yggU 1213795..1214097 (-) 303 WP_013050322.1 DUF167 family protein YggU -
  SVI_RS04895 (SVI_1041) - 1214104..1214652 (-) 549 WP_013050323.1 YggT family protein -
  SVI_RS04900 (SVI_1042) proC 1214728..1215546 (-) 819 WP_013050324.1 pyrroline-5-carboxylate reductase -
  SVI_RS04905 (SVI_1043) - 1215686..1216381 (-) 696 WP_013050325.1 YggS family pyridoxal phosphate-dependent enzyme -
  SVI_RS04910 (SVI_1044) pilT 1216418..1217455 (+) 1038 WP_013050326.1 type IV pilus twitching motility protein PilT Machinery gene
  SVI_RS04915 (SVI_1045) pilU 1217479..1218591 (+) 1113 WP_013050327.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SVI_RS04920 (SVI_1046) - 1218795..1219871 (+) 1077 WP_231847772.1 porin -
  SVI_RS04925 (SVI_1047) ruvX 1220269..1220691 (-) 423 WP_013050329.1 Holliday junction resolvase RuvX -
  SVI_RS04930 (SVI_1048) - 1220738..1221298 (-) 561 WP_013050330.1 YqgE/AlgH family protein -
  SVI_RS04935 (SVI_1049) yciH 1221380..1221709 (-) 330 WP_013050331.1 stress response translation initiation inhibitor YciH -
  SVI_RS04940 (SVI_1050) - 1221888..1222205 (-) 318 WP_013050332.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38321.91 Da        Isoelectric Point: 7.0819

>NTDB_id=36854 SVI_RS04910 WP_013050326.1 1216418..1217455(+) (pilT) [Shewanella violacea DSS12]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHGLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINESRHEHILTIED
PIEFVHQSKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
QTLVNRGQISREDAQHKSANKQTKF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=36854 SVI_RS04910 WP_013050326.1 1216418..1217455(+) (pilT) [Shewanella violacea DSS12]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAGCACAAAGCGTCAGATCTACACCTTTCAGCGGGCGTTTCGCCTAT
GATACGTGTTGATGGCGAAGTCAGAAAGATCAATTTACCCGCTTTAGATCATCAAGGGGTGCATGGACTCGTCTATGACA
TCATGAATGATAAGCAGCGTAAAGATTACGAAGAGCATTTAGAGATAGATTTTTCCTTCGAGGTACCAAATTTAGCGCGT
TTTCGAGTCAACGCTTTCAATCAGGCACGAGGAGCCGCGGCGGTATTTCGTACCATTCCCAGTGATATTCTGAGTTTAGA
GCAGCTTGGCGCACCGGAGATCTTCAAGAAAATATCCAGCTTCCCACGTGGCTTAGTCTTGGTAACCGGTCCTACAGGCT
CGGGAAAGAGTACCACATTGGCGGCCATGGTTGATTATATCAACGAGAGTCGTCACGAACACATTCTCACCATCGAAGAC
CCTATCGAATTTGTGCATCAGAGTAAGCAATGTCTGATAAACCAGCGTGAGGTTCACCGTCATACTCACAGTTTTAATGC
GGCTTTACGAAGCGCGCTGCGTGAAGATCCCGATGTTATCTTGGTGGGTGAGATGCGAGATCTTGAAACCATCAGGCTGG
CGATGACAGCAGCTGAGACCGGTCACTTGGTTTTCGGTACTCTACACACCACCTCAGCGGCAAAAACAATTGACCGTGTG
GTCGATGTTTTCCCCGAAGGCGAGAAGGGCATGGTTAGAACCATGCTATCTGAATCGTTACAGGCGGTAATATCGCAGAC
CCTAATTAAAAAAGTGGGAGGAGGGCGAGTGGCGGCCCATGAGATCATGATGGGAACACCGGCGATCAGGAACCTTATCC
GTGAGGATAAAGTGGCGCAGATGTATTCTGCGATTCAGACAGGTATGTCTCATGGCATGCAGACATTAGATCAATGCCTG
CAGACCTTGGTCAACCGGGGTCAGATCAGTCGTGAAGATGCTCAGCACAAGAGTGCCAATAAGCAGACAAAATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4ZH66

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.702

99.13

0.8
  pilT Acinetobacter baumannii D1279779

80.702

99.13

0.8
  pilT Acinetobacter baumannii strain A118

80.702

99.13

0.8
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Pseudomonas stutzeri DSM 10701

78.994

97.971

0.774
  pilT Acinetobacter baylyi ADP1

78.806

97.101

0.765
  pilT Legionella pneumophila strain Lp02

75.66

98.841

0.748
  pilT Legionella pneumophila strain ERS1305867

75.66

98.841

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.765

94.783

0.481
  pilU Vibrio cholerae strain A1552

41.983

99.42

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

40.179

97.391

0.391

Multiple sequence alignment