Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SVI_RS04915 Genome accession   NC_014012
Coordinates   1217479..1218591 (+) Length   370 a.a.
NCBI ID   WP_013050327.1    Uniprot ID   D4ZH67
Organism   Shewanella violacea DSS12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1212479..1223591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SVI_RS04885 (SVI_1039) - 1213210..1213644 (-) 435 WP_013050321.1 DUF4426 domain-containing protein -
  SVI_RS04890 (SVI_1040) yggU 1213795..1214097 (-) 303 WP_013050322.1 DUF167 family protein YggU -
  SVI_RS04895 (SVI_1041) - 1214104..1214652 (-) 549 WP_013050323.1 YggT family protein -
  SVI_RS04900 (SVI_1042) proC 1214728..1215546 (-) 819 WP_013050324.1 pyrroline-5-carboxylate reductase -
  SVI_RS04905 (SVI_1043) - 1215686..1216381 (-) 696 WP_013050325.1 YggS family pyridoxal phosphate-dependent enzyme -
  SVI_RS04910 (SVI_1044) pilT 1216418..1217455 (+) 1038 WP_013050326.1 type IV pilus twitching motility protein PilT Machinery gene
  SVI_RS04915 (SVI_1045) pilU 1217479..1218591 (+) 1113 WP_013050327.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SVI_RS04920 (SVI_1046) - 1218795..1219871 (+) 1077 WP_231847772.1 porin -
  SVI_RS04925 (SVI_1047) ruvX 1220269..1220691 (-) 423 WP_013050329.1 Holliday junction resolvase RuvX -
  SVI_RS04930 (SVI_1048) - 1220738..1221298 (-) 561 WP_013050330.1 YqgE/AlgH family protein -
  SVI_RS04935 (SVI_1049) yciH 1221380..1221709 (-) 330 WP_013050331.1 stress response translation initiation inhibitor YciH -
  SVI_RS04940 (SVI_1050) - 1221888..1222205 (-) 318 WP_013050332.1 hypothetical protein -
  SVI_RS04945 (SVI_1051) - 1222398..1222958 (+) 561 WP_041419698.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41062.98 Da        Isoelectric Point: 5.4340

>NTDB_id=36855 SVI_RS04915 WP_013050327.1 1217479..1218591(+) (pilU) [Shewanella violacea DSS12]
MDVRPFLKTMIDRKASDLFITAGFPPSAKIDGELTPLSETSFSPAQSLDFVESLMSDEQKKEFHQTRECNFAFALPDLGR
FRVSAFWQREASGCVMRRIETQIPDVDDLKLPVILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNANSRGHILTIED
PVEFVHEHRKCIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALAFAETGHLCMATLHANNANQALDRI
MNLVPESKHQQLLFDLSLNLRGIVAQQLVPTMDGKGRRAAIEILINTPRISSLIAKNELHSLKETMAKSNEQGMQTFDQA
LLTLYTSNEISYADALHHADSPNDLRLMIKLQSSEQSGSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=36855 SVI_RS04915 WP_013050327.1 1217479..1218591(+) (pilU) [Shewanella violacea DSS12]
ATGGATGTTCGTCCTTTCTTAAAAACTATGATAGACCGCAAGGCCTCGGATCTATTTATTACTGCGGGTTTCCCCCCGAG
TGCCAAAATAGATGGTGAGCTCACACCATTGAGTGAAACCTCATTCTCTCCGGCTCAGTCACTGGATTTTGTCGAGTCCT
TGATGAGCGATGAGCAGAAGAAGGAGTTTCATCAGACTCGTGAGTGTAATTTTGCATTTGCCTTGCCGGATTTGGGACGT
TTTCGTGTCAGTGCCTTTTGGCAGAGAGAAGCATCGGGTTGTGTCATGCGTCGTATCGAGACTCAGATCCCCGATGTCGA
TGATCTAAAATTGCCAGTGATCCTCAAAGATCTGGTGATGAGCAAACGTGGACTCATCATCATGGTAGGCGGAACCGGTA
CGGGTAAGTCAACATCCTTGGCTGCACTGGTGGGCTATCGAAATGCTAACTCTCGTGGCCATATTTTAACTATCGAAGAT
CCGGTTGAATTTGTTCACGAGCACAGAAAATGTATCATTACCCAGCGAGAAGTGGGCATAGATACCGAGTCATTCGATGC
CGCGCTTAAAAGTTCATTACGCCAAGCGCCTGATGTGATCTTAATCGGTGAAATTCGAACTCAAGAGACCATGGAGTTTG
CCCTAGCGTTTGCCGAAACGGGTCACCTATGTATGGCGACCTTGCACGCAAACAATGCTAACCAGGCGTTGGACCGTATC
ATGAACTTAGTGCCGGAGAGTAAGCATCAGCAGCTACTATTCGACCTTTCGTTAAACTTAAGGGGAATTGTAGCTCAACA
GTTAGTGCCGACCATGGACGGTAAAGGGCGACGTGCGGCCATTGAGATTCTGATTAATACGCCTCGTATTAGTAGCTTGA
TCGCTAAAAATGAGCTGCATTCGCTCAAGGAGACCATGGCTAAGTCTAATGAGCAAGGGATGCAGACCTTCGATCAGGCC
TTGTTGACGCTATATACTTCTAATGAGATTAGTTATGCCGATGCCTTACATCATGCGGATTCTCCAAACGATTTAAGGTT
GATGATTAAGCTGCAGAGCTCAGAGCAATCGGGTTCAGGCTTTATGGAAGGAGTGACACTGGATTTAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D4ZH67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.753

100

0.611
  pilU Acinetobacter baylyi ADP1

61.364

95.135

0.584
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.159

93.243

0.384
  pilT Vibrio cholerae strain A1552

43.26

86.216

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.26

86.216

0.373
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362

Multiple sequence alignment