Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FHQ07_RS11730 Genome accession   NZ_CP040871
Coordinates   2491957..2492994 (+) Length   345 a.a.
NCBI ID   WP_139716979.1    Uniprot ID   A0A5B7ZSX4
Organism   Thermomonas aquatica strain SY21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2486957..2497994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHQ07_RS11695 (FHQ07_11695) - 2487551..2487754 (+) 204 WP_139716973.1 dodecin family protein -
  FHQ07_RS11700 (FHQ07_11700) pdxH 2487805..2488398 (+) 594 WP_240703485.1 pyridoxamine 5'-phosphate oxidase -
  FHQ07_RS11705 (FHQ07_11705) - 2488410..2489207 (+) 798 WP_139718051.1 cobalamin-binding protein -
  FHQ07_RS11710 (FHQ07_11710) - 2489236..2489631 (+) 396 WP_139716975.1 DoxX family protein -
  FHQ07_RS11715 (FHQ07_11715) - 2489666..2490163 (-) 498 WP_168191554.1 DUF4426 domain-containing protein -
  FHQ07_RS11720 (FHQ07_11720) proC 2490177..2490995 (-) 819 WP_139716977.1 pyrroline-5-carboxylate reductase -
  FHQ07_RS11725 (FHQ07_11725) - 2490998..2491708 (-) 711 WP_139716978.1 YggS family pyridoxal phosphate-dependent enzyme -
  FHQ07_RS11730 (FHQ07_11730) pilT 2491957..2492994 (+) 1038 WP_139716979.1 type IV pilus twitching motility protein PilT Machinery gene
  FHQ07_RS11735 (FHQ07_11735) pilU 2493031..2494158 (+) 1128 WP_139716980.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FHQ07_RS11740 (FHQ07_11740) - 2494221..2494787 (+) 567 WP_139716981.1 YqgE/AlgH family protein -
  FHQ07_RS11745 (FHQ07_11745) ruvX 2494784..2495257 (+) 474 WP_139716982.1 Holliday junction resolvase RuvX -
  FHQ07_RS11750 (FHQ07_11750) - 2495254..2496201 (+) 948 WP_139716983.1 aspartate carbamoyltransferase catalytic subunit -
  FHQ07_RS11755 (FHQ07_11755) - 2496208..2497011 (-) 804 WP_139716984.1 prolyl oligopeptidase family serine peptidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38462.27 Da        Isoelectric Point: 6.8889

>NTDB_id=367757 FHQ07_RS11730 WP_139716979.1 2491957..2492994(+) (pilT) [Thermomonas aquatica strain SY21]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPAFDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSQVLTLEDLGCPRIFKELIEQPQGLILVTGPTGSGKSTTLAAMIDHINKSEYGHILSVED
PIEFVHTSQKCLINQREVHKDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQNAGMMTLDQHL
QDLVKRGMVPKQQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=367757 FHQ07_RS11730 WP_139716979.1 2491957..2492994(+) (pilT) [Thermomonas aquatica strain SY21]
ATGGATATCGCTGAGCTACTGGCGTTTTCGGTCAAGAACAAGGCATCGGACCTGCATCTGTCGGCGGGCTTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGCCGCATCAACATCCCGGCGTTCGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGACTACGAGGAATTCCTCGAAGTCGACTTCTCGTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCGCAGGTGCTGACCCTGGA
AGACCTGGGCTGCCCGCGCATCTTCAAGGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGTT
CCGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAGCGAATACGGGCACATCCTCTCGGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAATGCCTGATCAACCAGCGCGAAGTGCACAAGGACACCCACGGCTTCAACGA
GGCCCTGCGTTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGTTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGCCACCCTGCACACCTCGAGCGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGTTCCATGCTCTCCGAGTCGCTGCGCGCGGTGATCTCGCAGGC
CTTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCGTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGGCAGAACGCCGGCATGATGACGCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCGGCATGGTGCCCAAGCAGCAAGCGCGCGAATATGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7ZSX4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

77.581

98.261

0.762
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.851

97.101

0.678
  pilT Vibrio cholerae strain A1552

69.851

97.101

0.678
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Neisseria gonorrhoeae MS11

64.809

98.841

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

100

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.003

98.261

0.403
  pilU Vibrio cholerae strain A1552

39.655

100

0.4
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394

Multiple sequence alignment