Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FHQ07_RS11735 Genome accession   NZ_CP040871
Coordinates   2493031..2494158 (+) Length   375 a.a.
NCBI ID   WP_139716980.1    Uniprot ID   A0A5B7ZSD3
Organism   Thermomonas aquatica strain SY21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2488031..2499158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FHQ07_RS11705 (FHQ07_11705) - 2488410..2489207 (+) 798 WP_139718051.1 cobalamin-binding protein -
  FHQ07_RS11710 (FHQ07_11710) - 2489236..2489631 (+) 396 WP_139716975.1 DoxX family protein -
  FHQ07_RS11715 (FHQ07_11715) - 2489666..2490163 (-) 498 WP_168191554.1 DUF4426 domain-containing protein -
  FHQ07_RS11720 (FHQ07_11720) proC 2490177..2490995 (-) 819 WP_139716977.1 pyrroline-5-carboxylate reductase -
  FHQ07_RS11725 (FHQ07_11725) - 2490998..2491708 (-) 711 WP_139716978.1 YggS family pyridoxal phosphate-dependent enzyme -
  FHQ07_RS11730 (FHQ07_11730) pilT 2491957..2492994 (+) 1038 WP_139716979.1 type IV pilus twitching motility protein PilT Machinery gene
  FHQ07_RS11735 (FHQ07_11735) pilU 2493031..2494158 (+) 1128 WP_139716980.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FHQ07_RS11740 (FHQ07_11740) - 2494221..2494787 (+) 567 WP_139716981.1 YqgE/AlgH family protein -
  FHQ07_RS11745 (FHQ07_11745) ruvX 2494784..2495257 (+) 474 WP_139716982.1 Holliday junction resolvase RuvX -
  FHQ07_RS11750 (FHQ07_11750) - 2495254..2496201 (+) 948 WP_139716983.1 aspartate carbamoyltransferase catalytic subunit -
  FHQ07_RS11755 (FHQ07_11755) - 2496208..2497011 (-) 804 WP_139716984.1 prolyl oligopeptidase family serine peptidase -
  FHQ07_RS11760 (FHQ07_11760) mgtE 2497059..2498420 (-) 1362 WP_139716985.1 magnesium transporter -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41723.05 Da        Isoelectric Point: 6.6610

>NTDB_id=367758 FHQ07_RS11735 WP_139716980.1 2493031..2494158(+) (pilU) [Thermomonas aquatica strain SY21]
MSIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQAPLTPQQSKDLVLNVMTPQQREEFEKTHECNFAIGVSGV
GRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIITI
EDPIEFVHKHDGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMD
RIINFFPEDRRNQLLMDMSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTFD
QSLFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDASTLAQGLDGVEVAEAR

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=367758 FHQ07_RS11735 WP_139716980.1 2493031..2494158(+) (pilU) [Thermomonas aquatica strain SY21]
ATGAGCATCGACTTCACCTCATTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGGCGGGCATGCC
GCCGTCGATGAAGGTGCACGGCAAGATTTCGCCGATCACCCAGGCCCCGCTCACGCCGCAGCAGTCCAAGGACCTGGTGC
TGAACGTGATGACGCCGCAGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAACTTCGCGATCGGCGTGTCCGGCGTC
GGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACCCGCATCCCGAC
CGTCGAGGAGCTGAACCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCA
CCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACAGCACCGGCCACATCATCACCATC
GAGGATCCGATCGAGTTCGTGCACAAGCACGACGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAGCTG
GGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCATGG
ACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCGAACAACGCCAACCAGGCGATGGAC
CGCATCATCAACTTCTTCCCCGAGGACCGCCGCAACCAGCTGCTGATGGACATGTCGCTGAACCTGAAGGGCGTGGTCGC
GCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGGGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGCAGG
ACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAATCCGTGCAGCTGGGCATGAAGACCTTCGAC
CAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGATGCACTGCGCTACGCCGACTCCGCCAACGAGGT
GCGCCTGCGGATCAAGCTGGCGCAGGGCGGCGACGCCTCCACGCTGGCCCAGGGCCTGGATGGGGTCGAGGTCGCCGAAG
CGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7ZSD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.867

0.661
  pilU Acinetobacter baylyi ADP1

63.585

95.2

0.605
  pilU Vibrio cholerae strain A1552

54.213

94.933

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

90.4

0.381
  pilT Pseudomonas aeruginosa PAK

40.896

89.333

0.365

Multiple sequence alignment