Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NHAL_RS18390 Genome accession   NC_013960
Coordinates   3906692..3907729 (-) Length   345 a.a.
NCBI ID   WP_013034656.1    Uniprot ID   D5C3A0
Organism   Nitrosococcus halophilus Nc 4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3901692..3912729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHAL_RS18365 (Nhal_3787) - 3901929..3902807 (+) 879 WP_013034651.1 DMT family transporter -
  NHAL_RS18370 (Nhal_3788) - 3903037..3903420 (-) 384 WP_013034652.1 hypothetical protein -
  NHAL_RS18375 (Nhal_3789) - 3903562..3904254 (-) 693 WP_013034653.1 hypothetical protein -
  NHAL_RS18380 (Nhal_3790) - 3904694..3905506 (+) 813 WP_013034654.1 SDR family NAD(P)-dependent oxidoreductase -
  NHAL_RS18385 (Nhal_3791) pilU 3905541..3906683 (-) 1143 WP_013034655.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NHAL_RS18390 (Nhal_3792) pilT 3906692..3907729 (-) 1038 WP_013034656.1 type IV pilus twitching motility protein PilT Machinery gene
  NHAL_RS18395 (Nhal_3793) - 3907850..3908545 (+) 696 WP_013034657.1 YggS family pyridoxal phosphate-dependent enzyme -
  NHAL_RS18400 (Nhal_3794) proC 3908640..3909473 (+) 834 WP_013034658.1 pyrroline-5-carboxylate reductase -
  NHAL_RS18405 (Nhal_3795) - 3909466..3910029 (+) 564 WP_013034659.1 YggT family protein -
  NHAL_RS18410 (Nhal_3796) - 3910036..3910344 (+) 309 WP_013034660.1 DUF167 domain-containing protein -
  NHAL_RS18415 (Nhal_3797) - 3910387..3911610 (-) 1224 WP_013034661.1 AmpG family muropeptide MFS transporter -
  NHAL_RS18420 (Nhal_3798) pyrE 3911734..3912396 (+) 663 WP_013034662.1 orotate phosphoribosyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38325.04 Da        Isoelectric Point: 6.7303

>NTDB_id=36771 NHAL_RS18390 WP_013034656.1 3906692..3907729(-) (pilT) [Nitrosococcus halophilus Nc 4]
MDIAELLAFSVKNSASDLHISAGLPPMIRVDGDVRRINLPPMEHKEVHAMIYDIMNDKQRKGYEEFLETDFSFEIPGLAR
FRVNAFNQNRGAAAVFRTIPSQVLTLEELDAPAVFKDIANHPRGIVLVTGPTGSGKSTTLAAMVNYKNENDFAHILTIED
PIEFVHESKKSLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKFGGGRVAAHEIMIGTPAIRNLIREDKIPQMYSAIQTGQGAGMQTLDQCL
LELTRRNVVTKQEAVKKAVSKELFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=36771 NHAL_RS18390 WP_013034656.1 3906692..3907729(-) (pilT) [Nitrosococcus halophilus Nc 4]
ATGGATATCGCCGAACTGCTTGCCTTTAGTGTCAAGAATTCTGCCTCTGACTTACATATCTCTGCTGGACTCCCTCCGAT
GATCCGGGTAGATGGCGACGTCCGCCGGATCAATTTACCACCCATGGAGCACAAGGAAGTCCACGCCATGATTTACGATA
TTATGAATGACAAGCAGCGTAAGGGTTATGAAGAATTTCTTGAAACCGATTTTTCCTTCGAGATCCCTGGATTAGCCCGT
TTCCGCGTCAATGCCTTCAATCAAAACCGTGGGGCAGCGGCAGTATTCCGCACCATTCCCTCCCAGGTACTGACCCTCGA
GGAGCTGGATGCCCCCGCTGTGTTTAAGGATATCGCCAACCATCCTCGGGGGATTGTGTTGGTGACTGGACCTACCGGCT
CCGGAAAATCAACCACCTTAGCGGCCATGGTTAATTATAAAAACGAGAATGATTTTGCCCACATTTTAACTATCGAAGAT
CCTATTGAATTCGTTCATGAAAGCAAAAAAAGCCTTGTTAATCAACGTGAAGTTCATCGGGATACCCATGGTTTTAGCGA
AGCGCTACGCTCGGCATTACGTGAAGATCCTGACATCATCCTAGTGGGTGAGATGCGGGATCTGGAAACCATTCGTCTCG
CCTTAACGGCGGCTGAGACAGGCCACCTGGTTTTTGGCACCTTGCATACCAGTTCTGCGGCAAAGACTATTGACCGTATC
ATCGATGTATTCCCTGCGGCGGAGAAAGATATGGTGCGCTCAATGCTCTCCGAATCCTTGCGGGCGGTCATTTCTCAGAC
CCTCCTTAAAAAATTTGGCGGTGGTCGGGTTGCGGCCCACGAGATCATGATTGGCACTCCAGCCATCCGCAACCTCATTC
GTGAAGATAAAATCCCCCAAATGTATTCTGCTATCCAGACGGGCCAAGGAGCAGGCATGCAAACCTTGGATCAGTGCCTG
TTAGAGTTGACCCGCCGCAATGTGGTGACCAAACAGGAGGCAGTCAAGAAAGCAGTAAGCAAAGAACTTTTCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5C3A0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

69.388

99.42

0.69
  pilT Neisseria gonorrhoeae MS11

69.096

99.42

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.751

100

0.409
  pilU Vibrio cholerae strain A1552

39.884

100

0.4
  pilU Acinetobacter baylyi ADP1

38.617

100

0.388

Multiple sequence alignment