Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SLIT_RS13780 Genome accession   NC_013959
Coordinates   2786448..2787491 (+) Length   347 a.a.
NCBI ID   WP_013030882.1    Uniprot ID   D5CPI9
Organism   Sideroxydans lithotrophicus ES-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2781448..2792491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SLIT_RS13750 (Slit_2753) - 2782538..2782822 (-) 285 WP_013030876.1 HU family DNA-binding protein -
  SLIT_RS13755 (Slit_2754) - 2782994..2783587 (-) 594 WP_013030877.1 SRPBCC family protein -
  SLIT_RS13760 (Slit_2755) - 2783642..2783926 (-) 285 WP_013030878.1 DUF167 domain-containing protein -
  SLIT_RS13765 (Slit_2756) - 2783923..2784489 (-) 567 WP_013030879.1 YggT family protein -
  SLIT_RS13770 (Slit_2757) proC 2784634..2785446 (-) 813 WP_013030880.1 pyrroline-5-carboxylate reductase -
  SLIT_RS13775 (Slit_2758) - 2785715..2786395 (-) 681 WP_013030881.1 YggS family pyridoxal phosphate-dependent enzyme -
  SLIT_RS13780 (Slit_2759) pilT 2786448..2787491 (+) 1044 WP_013030882.1 type IV pilus twitching motility protein PilT Machinery gene
  SLIT_RS13785 (Slit_2760) pilU 2787534..2788670 (+) 1137 WP_013030883.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SLIT_RS13790 (Slit_2761) - 2788681..2789160 (+) 480 WP_013030884.1 hypothetical protein -
  SLIT_RS13795 (Slit_2762) - 2789171..2789620 (-) 450 WP_013030885.1 DoxX family protein -
  SLIT_RS13800 (Slit_2763) - 2789617..2790393 (-) 777 WP_013030886.1 DNA-binding domain-containing protein -
  SLIT_RS13805 (Slit_2764) - 2790380..2791228 (-) 849 WP_013030887.1 DUF692 domain-containing protein -
  SLIT_RS13810 (Slit_2765) - 2791228..2791494 (-) 267 WP_013030888.1 DUF2282 domain-containing protein -
  SLIT_RS13815 (Slit_2766) - 2791647..2792009 (-) 363 WP_013030889.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38376.30 Da        Isoelectric Point: 6.9317

>NTDB_id=36749 SLIT_RS13780 WP_013030882.1 2786448..2787491(+) (pilT) [Sideroxydans lithotrophicus ES-1]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDMRRINLPAMEHKEVHAMVYDIMNDGQRKHYEENKEVDFSFEVPNLAR
FRVNAFIQNRGAGAVMRTIPSKILTLEDLKCPPIFKDISEFPRGMVLVTGPTGSGKSTTLAAMVNHINENEMGHILTVED
PIEFVHESKKSLVNQREVGPHTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGSGRVAAHEIMICTPAIRNLIREAKVPQMYSAIQTGGNIGMQTLDQC
LQDLVKRNMVASSEARGKAANKDLFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=36749 SLIT_RS13780 WP_013030882.1 2786448..2787491(+) (pilT) [Sideroxydans lithotrophicus ES-1]
ATGGATATCACCGAACTGCTCGCCTTCGGCGTGAAGAACAAAGCCTCCGACTTGCACCTCTCCGCCGGCCTGCCTCCGAT
GATCCGCGTGCACGGCGACATGCGCCGCATCAACCTGCCAGCGATGGAGCACAAGGAAGTCCACGCCATGGTGTACGACA
TCATGAACGACGGCCAGCGCAAACATTACGAAGAGAACAAGGAAGTCGACTTCTCGTTCGAGGTGCCCAACCTGGCGCGT
TTCCGCGTCAATGCCTTCATCCAGAACCGCGGCGCCGGCGCGGTGATGCGTACCATTCCTTCCAAGATCCTCACGCTGGA
AGACCTCAAGTGCCCGCCGATCTTCAAGGACATCTCCGAATTCCCGCGCGGCATGGTGCTGGTGACCGGCCCGACCGGTT
CCGGTAAATCGACCACGCTGGCGGCGATGGTGAACCACATCAACGAGAACGAGATGGGCCACATCCTGACCGTGGAAGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGTCGCTGGTCAACCAGCGCGAAGTCGGCCCGCATACACTGTCGTTCAACAA
CGCCTTGCGTTCCGCACTGCGCGAGGACCCGGACGTGATCCTCGTCGGCGAAATGCGCGACCTCGAGACCATCCGTCTGG
CGATGACGGCGGCGGAAACCGGCCACCTGGTGTTCGGTACCTTGCACACCAGCTCGGCAGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCCGCCGACGAAAAGGAAATGGTGCGCGCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
CCTGCTCAAGACCAAGGACGGCAGCGGTCGCGTGGCGGCGCACGAGATCATGATCTGTACCCCGGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGCCGCAGATGTATTCCGCCATCCAGACCGGCGGCAACATCGGCATGCAGACACTGGACCAGTGC
CTGCAGGACCTGGTGAAGCGCAACATGGTGGCATCTTCCGAAGCGCGCGGCAAAGCGGCGAACAAGGACCTGTTCCCCGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5CPI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Pseudomonas stutzeri DSM 10701

74.203

99.424

0.738
  pilT Pseudomonas aeruginosa PAK

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

72.754

99.424

0.723
  pilT Legionella pneumophila strain ERS1305867

72.754

99.424

0.723
  pilT Neisseria meningitidis 8013

72.303

98.847

0.715
  pilT Neisseria gonorrhoeae MS11

72.012

98.847

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.542

0.412
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

41.071

96.83

0.398

Multiple sequence alignment