Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SLIT_RS13785 Genome accession   NC_013959
Coordinates   2787534..2788670 (+) Length   378 a.a.
NCBI ID   WP_013030883.1    Uniprot ID   D5CPJ0
Organism   Sideroxydans lithotrophicus ES-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2782534..2793670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SLIT_RS13750 (Slit_2753) - 2782538..2782822 (-) 285 WP_013030876.1 HU family DNA-binding protein -
  SLIT_RS13755 (Slit_2754) - 2782994..2783587 (-) 594 WP_013030877.1 SRPBCC family protein -
  SLIT_RS13760 (Slit_2755) - 2783642..2783926 (-) 285 WP_013030878.1 DUF167 domain-containing protein -
  SLIT_RS13765 (Slit_2756) - 2783923..2784489 (-) 567 WP_013030879.1 YggT family protein -
  SLIT_RS13770 (Slit_2757) proC 2784634..2785446 (-) 813 WP_013030880.1 pyrroline-5-carboxylate reductase -
  SLIT_RS13775 (Slit_2758) - 2785715..2786395 (-) 681 WP_013030881.1 YggS family pyridoxal phosphate-dependent enzyme -
  SLIT_RS13780 (Slit_2759) pilT 2786448..2787491 (+) 1044 WP_013030882.1 type IV pilus twitching motility protein PilT Machinery gene
  SLIT_RS13785 (Slit_2760) pilU 2787534..2788670 (+) 1137 WP_013030883.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SLIT_RS13790 (Slit_2761) - 2788681..2789160 (+) 480 WP_013030884.1 hypothetical protein -
  SLIT_RS13795 (Slit_2762) - 2789171..2789620 (-) 450 WP_013030885.1 DoxX family protein -
  SLIT_RS13800 (Slit_2763) - 2789617..2790393 (-) 777 WP_013030886.1 DNA-binding domain-containing protein -
  SLIT_RS13805 (Slit_2764) - 2790380..2791228 (-) 849 WP_013030887.1 DUF692 domain-containing protein -
  SLIT_RS13810 (Slit_2765) - 2791228..2791494 (-) 267 WP_013030888.1 DUF2282 domain-containing protein -
  SLIT_RS13815 (Slit_2766) - 2791647..2792009 (-) 363 WP_013030889.1 hypothetical protein -
  SLIT_RS13820 (Slit_2767) msrA 2792023..2792649 (-) 627 WP_013030890.1 peptide-methionine (S)-S-oxide reductase MsrA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42057.26 Da        Isoelectric Point: 6.7406

>NTDB_id=36750 SLIT_RS13785 WP_013030883.1 2787534..2788670(+) (pilU) [Sideroxydans lithotrophicus ES-1]
MERDQATELVHNLLRGLVSQKASDLFITSGFPPAFKIDGKMTPVSSQALTSQHTQELARSIMNDRQAAEFESTHECNFAI
SPPGIGRFRVNVFMQQQRVGMVLRTITTKIPSFEELGLPKVLKDVVMTKRGLAILVGGTGSGKSTSLAAMLGHRNHNSFG
HIITIEDPVEFVHDHDKCIVTHREVGVDTNDWHTALKNTLRQAPDVILIGEIRDRETMEYAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERREQLLMDLSLNIKALISQRLIPKKDGKGRAAAFEILLNSPLIADLIFKGAVHEIKEVMARSRELG
MQTFDQALFDLHEAGAITYEEALKNADSVNDLRLKIKLEGQATRDRDVMSGLDNLKMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=36750 SLIT_RS13785 WP_013030883.1 2787534..2788670(+) (pilU) [Sideroxydans lithotrophicus ES-1]
ATGGAAAGAGACCAGGCCACCGAACTCGTCCACAACCTGCTGCGCGGACTGGTTTCGCAGAAGGCGTCCGACCTGTTCAT
CACCTCAGGCTTCCCGCCCGCTTTCAAGATCGACGGCAAGATGACGCCGGTCTCCAGCCAGGCGCTGACCTCGCAGCACA
CGCAGGAGCTGGCGCGCAGCATCATGAACGACCGCCAGGCAGCCGAGTTCGAATCCACGCACGAATGCAACTTCGCCATC
AGCCCGCCCGGCATCGGGCGCTTCCGCGTCAATGTGTTCATGCAGCAACAGCGCGTCGGCATGGTATTGCGTACCATCAC
CACCAAGATCCCGTCCTTTGAAGAACTGGGACTGCCGAAAGTGCTCAAGGACGTGGTGATGACCAAACGCGGACTGGCGA
TCCTGGTCGGCGGTACCGGTTCCGGCAAATCCACCTCGCTGGCCGCCATGCTTGGTCACCGCAACCACAACAGCTTCGGC
CACATCATCACCATCGAGGACCCGGTGGAGTTCGTGCACGATCACGACAAGTGCATCGTCACCCACCGCGAAGTGGGCGT
GGATACCAACGACTGGCACACCGCATTGAAGAACACCTTGCGCCAGGCGCCCGACGTCATCCTGATCGGCGAGATCCGCG
ACCGCGAGACCATGGAATATGCCGTGGCATTCGCCGAAACCGGCCACCTTTGTCTGGCGACGCTGCACGCCAACAGTGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCGGAAGAACGCCGCGAACAGTTGCTGATGGACCTGTCGCTCAATAT
CAAGGCACTCATCTCGCAACGCCTGATCCCGAAGAAGGACGGCAAGGGACGCGCAGCCGCTTTCGAGATCCTGCTCAATT
CGCCGCTGATCGCGGACCTCATCTTCAAGGGCGCCGTGCATGAGATCAAGGAAGTCATGGCCAGGTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGCACGAAGCCGGTGCCATCACTTACGAAGAAGCCCTCAAGAACGCCGA
CTCGGTCAACGACCTGCGCCTGAAGATCAAGCTGGAAGGCCAGGCCACCCGGGACAGGGATGTGATGAGCGGGCTGGACA
ATCTGAAGATGACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D5CPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.162

91.534

0.587
  pilU Acinetobacter baylyi ADP1

58.065

98.413

0.571
  pilU Vibrio cholerae strain A1552

55.285

97.619

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

42.478

89.683

0.381
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Acinetobacter nosocomialis M2

42.042

88.095

0.37
  pilT Acinetobacter baumannii D1279779

42.042

88.095

0.37
  pilT Acinetobacter baumannii strain A118

42.042

88.095

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.641

87.037

0.362
  pilT Vibrio cholerae strain A1552

41.641

87.037

0.362

Multiple sequence alignment