Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FE240_RS13775 Genome accession   NZ_CP040449
Coordinates   2910242..2911276 (-) Length   344 a.a.
NCBI ID   WP_193001722.1    Uniprot ID   A0A5J6WX02
Organism   Aeromonas simiae strain A6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2905242..2916276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE240_RS13745 (FE240_13745) nudC 2905337..2906137 (-) 801 WP_404800029.1 NAD(+) diphosphatase -
  FE240_RS13750 (FE240_13750) rsd 2906287..2906778 (+) 492 WP_193001714.1 sigma D regulator -
  FE240_RS13755 (FE240_13755) - 2906879..2907271 (+) 393 WP_193004564.1 PilZ domain-containing protein -
  FE240_RS13760 (FE240_13760) - 2907472..2907867 (+) 396 WP_193001716.1 PilZ domain-containing protein -
  FE240_RS13765 (FE240_13765) yaaA 2908316..2909092 (-) 777 WP_193001718.1 peroxide stress protein YaaA -
  FE240_RS13770 (FE240_13770) pilU 2909107..2910225 (-) 1119 WP_193001720.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FE240_RS13775 (FE240_13775) pilT 2910242..2911276 (-) 1035 WP_193001722.1 type IVa pilus ATPase TapT Machinery gene
  FE240_RS13780 (FE240_13780) - 2911316..2912017 (+) 702 WP_193001724.1 YggS family pyridoxal phosphate-dependent enzyme -
  FE240_RS13785 (FE240_13785) - 2912078..2912629 (+) 552 WP_193001726.1 YggT family protein -
  FE240_RS13790 (FE240_13790) yggU 2912629..2912919 (+) 291 WP_193001728.1 DUF167 family protein YggU -
  FE240_RS13795 (FE240_13795) - 2912939..2913355 (+) 417 WP_193001730.1 DUF4426 domain-containing protein -
  FE240_RS13800 (FE240_13800) - 2913390..2913806 (-) 417 WP_193001732.1 hypothetical protein -
  FE240_RS13805 (FE240_13805) hupA 2913920..2914192 (-) 273 WP_042047881.1 nucleoid-associated protein HU-alpha -
  FE240_RS13810 (FE240_13810) rpoS 2914432..2915418 (-) 987 WP_193001736.1 RNA polymerase sigma factor RpoS -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38473.20 Da        Isoelectric Point: 6.6711

>NTDB_id=364561 FE240_RS13775 WP_193001722.1 2910242..2911276(-) (pilT) [Aeromonas simiae strain A6]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENYEVDFSFEIPGMAR
FRVNAFQHSRGAGAVFRTIPSVVLSLEDLEAPEIFRKISEFPRGLVLVTGPTGSGKSTTLAAMINYINENFHHHILTIED
PIEFVHENKRCLINQREVHRDTRSFSNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKRLGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQNL
KQLVARGIVAQLDARTKATDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=364561 FE240_RS13775 WP_193001722.1 2910242..2911276(-) (pilT) [Aeromonas simiae strain A6]
ATGGATATCACAGAGTTACTCGCCTTCAGTGTAAAGCATAAAGCGTCGGATCTACACCTTTCGGCCGGCGTTCCCCCGAT
GATCCGGGTTGATGGCGAAGTCCGCAAGATTAACTTACCTGCGCTGGAGCATCGGGAAGTTCACGCCCTCATCTATGACA
TCATGAATGATCATCAGCGCAAGGAGCTTGAGGAGAACTATGAAGTCGACTTCTCCTTCGAGATCCCCGGCATGGCCCGC
TTCCGGGTTAACGCTTTTCAGCACTCCCGCGGGGCCGGTGCGGTCTTTCGTACCATCCCCAGTGTGGTTCTGAGTCTGGA
GGATTTGGAAGCACCTGAGATTTTCCGCAAGATCTCCGAGTTCCCGAGAGGCCTAGTGCTGGTGACCGGGCCGACCGGAT
CGGGCAAGTCCACGACGCTGGCGGCCATGATCAACTACATCAACGAGAACTTCCACCACCATATCCTCACCATTGAGGAT
CCGATCGAATTCGTGCATGAGAACAAGCGCTGCCTTATCAACCAGCGTGAGGTGCATCGCGATACGCGCAGCTTCAGTAA
CGCCTTGCGTTCGGCGCTGCGGGAAGACCCGGACATCATACTGGTGGGGGAGTTGCGTGACCTTGAAACCATTCGACTGG
CCCTGACGGCCGCTGAGACCGGTCACTTGGTGTTCGGTACCCTGCACACCTCATCGGCAGCCAAGACCATCGACCGTATC
ATCGACGTCTTTCCCGGCGAGGAGAAGGACATGGTGCGCTCCATGCTTTCCGAGTCGCTACGCGCCGTTATCTCCCAGAC
ACTGCTCAAGCGTCTCGGTGGGGGGCGAGTGGCCGCCCATGAGATCATGCTCGGCATCCCGGCGGTACGAAACCTGATCC
GTGAGGACAAGGTGGCTCAGCTCTATTCGGTGATCCAGACCGGTATGACCCATGGCATGCAGACCATGGATCAAAACCTC
AAACAGTTGGTAGCGCGCGGTATCGTGGCTCAACTCGATGCCAGAACCAAGGCCACCGACCCTAACAGTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6WX02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.616

100

0.776
  pilT Acinetobacter baumannii strain A118

77.616

100

0.776
  pilT Acinetobacter baumannii D1279779

77.616

100

0.776
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Pseudomonas stutzeri DSM 10701

76.453

100

0.765
  pilT Pseudomonas aeruginosa PAK

76.163

100

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Neisseria meningitidis 8013

69.275

100

0.695
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.603

99.709

0.515
  pilU Vibrio cholerae strain A1552

42.029

100

0.422
  pilU Pseudomonas stutzeri DSM 10701

40.87

100

0.41
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment