Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FE240_RS13770 Genome accession   NZ_CP040449
Coordinates   2909107..2910225 (-) Length   372 a.a.
NCBI ID   WP_193001720.1    Uniprot ID   A0A5J6X1U6
Organism   Aeromonas simiae strain A6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2904107..2915225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE240_RS13740 (FE240_13740) hemE 2904182..2905249 (-) 1068 WP_042047896.1 uroporphyrinogen decarboxylase -
  FE240_RS13745 (FE240_13745) nudC 2905337..2906137 (-) 801 WP_404800029.1 NAD(+) diphosphatase -
  FE240_RS13750 (FE240_13750) rsd 2906287..2906778 (+) 492 WP_193001714.1 sigma D regulator -
  FE240_RS13755 (FE240_13755) - 2906879..2907271 (+) 393 WP_193004564.1 PilZ domain-containing protein -
  FE240_RS13760 (FE240_13760) - 2907472..2907867 (+) 396 WP_193001716.1 PilZ domain-containing protein -
  FE240_RS13765 (FE240_13765) yaaA 2908316..2909092 (-) 777 WP_193001718.1 peroxide stress protein YaaA -
  FE240_RS13770 (FE240_13770) pilU 2909107..2910225 (-) 1119 WP_193001720.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FE240_RS13775 (FE240_13775) pilT 2910242..2911276 (-) 1035 WP_193001722.1 type IVa pilus ATPase TapT Machinery gene
  FE240_RS13780 (FE240_13780) - 2911316..2912017 (+) 702 WP_193001724.1 YggS family pyridoxal phosphate-dependent enzyme -
  FE240_RS13785 (FE240_13785) - 2912078..2912629 (+) 552 WP_193001726.1 YggT family protein -
  FE240_RS13790 (FE240_13790) yggU 2912629..2912919 (+) 291 WP_193001728.1 DUF167 family protein YggU -
  FE240_RS13795 (FE240_13795) - 2912939..2913355 (+) 417 WP_193001730.1 DUF4426 domain-containing protein -
  FE240_RS13800 (FE240_13800) - 2913390..2913806 (-) 417 WP_193001732.1 hypothetical protein -
  FE240_RS13805 (FE240_13805) hupA 2913920..2914192 (-) 273 WP_042047881.1 nucleoid-associated protein HU-alpha -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41358.50 Da        Isoelectric Point: 5.0535

>NTDB_id=364560 FE240_RS13770 WP_193001720.1 2909107..2910225(-) (pilU) [Aeromonas simiae strain A6]
MSMEAWLSRLVDEQGSDLFITVGRAPTVKCSGLFISLTEAPLDLEQVRQAMVSVMDSQVLERFERQREANFAISRPGLGR
FRVSAFWQQDVPGMVLRRIQMQIPTCEELNLPTILKEIVMAKRGLILFVGATGVGKSTTQAAMIGYRNAHGDGHILTVED
PVEFVHQHGRCIVTQREVGSDTLSFDEGLRSSLRQAPDLILIGEIRSPETMEFAIQFAETGHICMATLHANNADQAIERI
MHLIAEERHRQLRYDLAFNLKAIVAQQLLPLSDGSGRCAAFEILLNTPLVADIIRKGEVDRLKEVMGRSREQGMLTFDQS
LFELYMAGRIGYEDALAYADSANDLRLMIKLGGGEKLGPGTLDNVTLQQDAD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=364560 FE240_RS13770 WP_193001720.1 2909107..2910225(-) (pilU) [Aeromonas simiae strain A6]
ATGAGCATGGAAGCTTGGTTATCACGTCTTGTCGACGAGCAGGGATCAGACCTATTTATCACAGTGGGTCGGGCTCCGAC
CGTAAAATGCAGTGGTCTCTTCATATCGTTAACGGAAGCGCCACTCGATCTGGAGCAGGTACGCCAAGCGATGGTCAGCG
TGATGGACTCCCAGGTGCTGGAGCGCTTTGAGCGCCAACGTGAGGCCAACTTTGCCATCTCTCGTCCCGGATTGGGGCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGATGTTCCTGGCATGGTGCTGCGGCGAATACAGATGCAGATCCCGACCTGTGA
AGAGCTGAACCTGCCCACCATCCTCAAGGAGATTGTGATGGCCAAGCGGGGGCTCATTCTCTTTGTCGGGGCGACCGGGG
TCGGCAAGTCGACCACTCAGGCGGCCATGATTGGTTATCGTAACGCCCATGGCGACGGGCACATCCTGACGGTGGAAGAT
CCGGTCGAGTTCGTCCACCAGCATGGGCGTTGTATCGTGACCCAGCGCGAAGTGGGAAGCGATACGCTCTCGTTCGATGA
GGGGCTGCGCAGCTCCTTACGTCAGGCGCCCGATCTCATCTTGATTGGCGAAATCCGCTCACCCGAGACCATGGAATTTG
CCATCCAGTTTGCCGAAACCGGGCATATCTGCATGGCGACGCTGCATGCTAACAATGCGGATCAGGCCATTGAGCGGATC
ATGCACCTCATTGCGGAAGAGCGGCATCGCCAATTACGTTACGATCTGGCTTTTAACCTCAAAGCCATTGTCGCTCAGCA
GTTGTTGCCACTTTCGGATGGCAGCGGGCGCTGCGCGGCCTTTGAGATCCTGCTCAATACCCCGCTCGTGGCTGATATCA
TCCGTAAGGGAGAGGTCGATCGCCTGAAAGAGGTGATGGGACGCTCACGGGAACAGGGAATGCTCACCTTTGACCAGTCG
CTTTTCGAGCTTTATATGGCTGGCCGAATTGGTTATGAGGATGCCTTGGCCTATGCCGATTCCGCCAATGATCTGCGCCT
GATGATCAAACTGGGTGGGGGCGAGAAACTTGGCCCGGGAACATTGGATAATGTTACCCTGCAACAAGACGCCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6X1U6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.778

96.774

0.559
  pilU Vibrio cholerae strain A1552

55.858

98.656

0.551
  pilU Acinetobacter baylyi ADP1

53.258

94.892

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.073

88.441

0.39
  pilT Acinetobacter baylyi ADP1

39.883

91.667

0.366

Multiple sequence alignment