Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TK90_RS12120 Genome accession   NC_013889
Coordinates   2537380..2538414 (-) Length   344 a.a.
NCBI ID   WP_012983776.1    Uniprot ID   D3SE77
Organism   Thioalkalivibrio sp. K90mix     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2532380..2543414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK90_RS12100 (TK90_2410) - 2532398..2533693 (+) 1296 WP_012983772.1 dihydroorotase -
  TK90_RS12105 (TK90_2411) - 2534001..2534906 (+) 906 WP_012983773.1 dihydroorotate dehydrogenase electron transfer subunit -
  TK90_RS12110 (TK90_2412) - 2535018..2536136 (-) 1119 WP_012983774.1 PilT/PilU family type 4a pilus ATPase -
  TK90_RS12115 (TK90_2413) pilU 2536164..2537336 (-) 1173 WP_012983775.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TK90_RS12120 (TK90_2414) pilT 2537380..2538414 (-) 1035 WP_012983776.1 type IV pilus twitching motility protein PilT Machinery gene
  TK90_RS12125 (TK90_2415) - 2538558..2539271 (+) 714 WP_012983777.1 YggS family pyridoxal phosphate-dependent enzyme -
  TK90_RS12130 (TK90_2416) proC 2539285..2540103 (+) 819 WP_012983778.1 pyrroline-5-carboxylate reductase -
  TK90_RS12135 (TK90_2417) cysQ 2540988..2541821 (-) 834 WP_012983779.1 3'(2'),5'-bisphosphate nucleotidase CysQ -
  TK90_RS12140 (TK90_2418) nudE 2541880..2542425 (-) 546 WP_012983780.1 ADP compounds hydrolase NudE -
  TK90_RS12145 (TK90_2419) - 2542433..2543146 (-) 714 WP_020146138.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38074.51 Da        Isoelectric Point: 6.3493

>NTDB_id=36425 TK90_RS12120 WP_012983776.1 2537380..2538414(-) (pilT) [Thioalkalivibrio sp. K90mix]
MDITQLLAFAVKNGASDLHISAGMPPMVRIDGDMRRVNVPVMDHKEVQGLIYDIMNDRQRKDYEEFLETDFSFEVTGLAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELAAPRIFEDISNYPRGIVLVTGPTGSGKSTTLAGMVNHRNENDYGHILTIED
PIEFVHESKKSLVNQREVHRDTHGFSEALRSALREDPDTILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKDMVRAMLSESLRAVIAQTLMKKIGGGRIAAHEIMLGTPAIRNLIRENKIAQMYSTIQTSAGTGMQTLDQCL
KDMLAQGVISREDAKRKAANPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=36425 TK90_RS12120 WP_012983776.1 2537380..2538414(-) (pilT) [Thioalkalivibrio sp. K90mix]
ATGGATATCACGCAGCTGCTGGCCTTTGCGGTCAAGAACGGGGCCTCGGATCTGCACATCTCGGCGGGCATGCCGCCGAT
GGTGCGCATCGACGGCGATATGCGCCGCGTGAATGTGCCGGTCATGGACCACAAGGAGGTCCAGGGCCTGATCTACGACA
TCATGAACGACCGCCAGCGCAAGGACTACGAGGAGTTCCTGGAGACGGACTTCTCGTTCGAGGTCACCGGGCTGGCGCGT
TTTCGTGTCAATGCGTTCAATCAGGACCGGGGCGCGGCGGCGGTCTTTCGTACCATCCCCTCCAAGGTGCTCACGCTGGA
AGAGCTGGCGGCACCGCGCATCTTCGAGGATATCTCCAACTATCCGCGCGGGATCGTGCTGGTCACGGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGGCATGGTCAACCACCGCAACGAGAATGACTACGGCCACATCCTGACGATCGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTCGCTGGTCAACCAGCGCGAGGTACACCGCGACACCCACGGCTTTTCCGA
GGCGCTGCGTTCCGCGCTGCGCGAGGATCCGGACACCATTCTGGTCGGCGAGCTGCGCGATCTCGAGACGATCCGCCTGG
CGCTGACCGCGGCGGAGACGGGGCACCTCGTCTTCGGCACCCTGCATACCAGTTCCGCGGCCAAGACGATCGACCGTATC
GTCGATGTGTTTCCGGCGGCCGAGAAGGACATGGTGCGGGCGATGTTGTCCGAGTCGCTGCGGGCGGTTATCGCGCAGAC
CCTGATGAAGAAGATCGGCGGCGGGCGTATCGCCGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGCAACCTGATCC
GCGAAAACAAGATCGCGCAGATGTACTCGACCATCCAGACCAGTGCCGGCACTGGCATGCAGACGCTGGACCAGTGCCTG
AAAGACATGCTGGCGCAGGGCGTGATCAGCCGCGAGGACGCCAAGCGCAAGGCCGCCAACCCGGATTCGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3SE77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

77.326

100

0.773
  pilT Pseudomonas stutzeri DSM 10701

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

77.035

100

0.77
  pilT Acinetobacter baylyi ADP1

75.872

100

0.759
  pilT Legionella pneumophila strain Lp02

70.058

100

0.701
  pilT Legionella pneumophila strain ERS1305867

70.058

100

0.701
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.965

0.683
  pilT Vibrio cholerae strain A1552

69.733

97.965

0.683
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.5
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401

Multiple sequence alignment