Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TK90_RS12115 Genome accession   NC_013889
Coordinates   2536164..2537336 (-) Length   390 a.a.
NCBI ID   WP_012983775.1    Uniprot ID   D3SE76
Organism   Thioalkalivibrio sp. K90mix     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531164..2542336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK90_RS12095 (TK90_2409) - 2531379..2532401 (+) 1023 WP_012983771.1 aspartate carbamoyltransferase catalytic subunit -
  TK90_RS12100 (TK90_2410) - 2532398..2533693 (+) 1296 WP_012983772.1 dihydroorotase -
  TK90_RS12105 (TK90_2411) - 2534001..2534906 (+) 906 WP_012983773.1 dihydroorotate dehydrogenase electron transfer subunit -
  TK90_RS12110 (TK90_2412) - 2535018..2536136 (-) 1119 WP_012983774.1 PilT/PilU family type 4a pilus ATPase -
  TK90_RS12115 (TK90_2413) pilU 2536164..2537336 (-) 1173 WP_012983775.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TK90_RS12120 (TK90_2414) pilT 2537380..2538414 (-) 1035 WP_012983776.1 type IV pilus twitching motility protein PilT Machinery gene
  TK90_RS12125 (TK90_2415) - 2538558..2539271 (+) 714 WP_012983777.1 YggS family pyridoxal phosphate-dependent enzyme -
  TK90_RS12130 (TK90_2416) proC 2539285..2540103 (+) 819 WP_012983778.1 pyrroline-5-carboxylate reductase -
  TK90_RS12135 (TK90_2417) cysQ 2540988..2541821 (-) 834 WP_012983779.1 3'(2'),5'-bisphosphate nucleotidase CysQ -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43831.93 Da        Isoelectric Point: 5.4919

>NTDB_id=36424 TK90_RS12115 WP_012983775.1 2536164..2537336(-) (pilU) [Thioalkalivibrio sp. K90mix]
MDRDKAIKYMHDLLRALVDRGGSDLFITVGMPPAAKIDGRVVPMTEQKLTPSHTQALVRALMNDKQSAEYERHQECNFAI
GLQGVSRFRVNVFTQRGSPGMVLRTINSVIPKFEDIQMPPQLEKITMERRGLVIFVGGTGSGKSTSLAAMIGHRNRNAPD
HIVTIEDPIEFVHEHEKSIITQREVGVDTEGYEIALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCLSTLHANST
NQALDRIINFFPEDRREQLLMDLSLNLKAVISQRLVRQENGEGRVPAVEILINTPLMADLMLKGAVHEMKELISRSREQG
MQTFDQALFDLYETRKISYEEALRNADSVNDLRLRIKLDSQRAQDDGAPAVADTGEGLSVDDRPEDYSVR

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=36424 TK90_RS12115 WP_012983775.1 2536164..2537336(-) (pilU) [Thioalkalivibrio sp. K90mix]
ATGGATCGTGACAAGGCGATCAAGTACATGCACGACCTGCTCCGCGCCCTGGTGGACCGGGGTGGCTCGGACCTGTTCAT
CACGGTCGGCATGCCGCCAGCGGCCAAGATTGACGGCCGCGTGGTGCCGATGACCGAGCAGAAGCTCACGCCTTCGCATA
CCCAGGCGCTGGTGCGCGCACTGATGAACGACAAGCAGTCCGCGGAGTACGAGCGCCATCAGGAGTGCAATTTCGCGATC
GGCCTGCAGGGCGTGTCGCGCTTTCGCGTGAACGTGTTTACTCAGCGCGGCAGCCCCGGAATGGTGCTGCGCACGATCAA
CTCGGTGATCCCGAAGTTCGAGGACATCCAGATGCCGCCGCAGCTGGAGAAGATCACCATGGAGCGCCGCGGGCTGGTGA
TCTTCGTCGGGGGGACCGGCTCGGGCAAGTCGACCTCGCTCGCCGCGATGATCGGCCATCGCAACCGCAATGCCCCCGAT
CACATCGTCACCATCGAGGACCCGATCGAGTTCGTCCACGAACACGAAAAGAGCATCATCACCCAGCGCGAGGTGGGCGT
GGACACCGAGGGCTACGAGATCGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGGG
ATCGGGAGACGATGGACTACGCGATCGCCTTTGCCGAGACCGGTCACCTGTGCCTGTCCACCCTGCACGCCAACAGCACC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCCGAAGACCGGCGCGAGCAGTTGCTGATGGACCTGTCGCTGAACCT
CAAGGCGGTGATCTCGCAGCGCCTGGTGCGGCAGGAGAATGGCGAGGGCCGTGTGCCGGCGGTAGAAATCCTGATCAACA
CCCCGTTGATGGCCGACCTGATGCTGAAGGGTGCCGTGCACGAGATGAAGGAGCTGATTTCGCGCTCCCGCGAGCAGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACCTCTACGAGACCCGCAAGATCAGCTACGAGGAGGCCCTGCGCAACGCCGA
TTCGGTGAATGACCTGCGGCTGCGCATCAAGCTCGACTCGCAGCGTGCACAGGATGACGGTGCACCGGCAGTGGCCGACA
CGGGCGAGGGCCTGTCCGTGGACGACCGTCCCGAGGACTACAGTGTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3SE76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.4

96.154

0.562
  pilU Acinetobacter baylyi ADP1

56.941

90.513

0.515
  pilU Vibrio cholerae strain A1552

52.198

93.333

0.487
  pilT Acinetobacter nosocomialis M2

43.505

84.872

0.369
  pilT Acinetobacter baumannii D1279779

43.505

84.872

0.369
  pilT Acinetobacter baumannii strain A118

43.505

84.872

0.369
  pilT Pseudomonas stutzeri DSM 10701

42.985

85.897

0.369
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.388

85.897

0.364
  pilT Pseudomonas aeruginosa PAK

42.09

85.897

0.362

Multiple sequence alignment