Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FCN25_RS03995 Genome accession   NZ_CP039928
Coordinates   933576..934613 (-) Length   345 a.a.
NCBI ID   WP_137222174.1    Uniprot ID   -
Organism   Shewanella sp. MEBiC00475     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 928576..939613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCN25_RS03970 yciH 928600..928929 (-) 330 WP_137222164.1 stress response translation initiation inhibitor YciH -
  FCN25_RS03975 hemH 929189..930202 (-) 1014 WP_137222166.1 ferrochelatase -
  FCN25_RS03980 - 930457..930990 (-) 534 WP_137222168.1 glutathione peroxidase -
  FCN25_RS03985 - 931100..932203 (-) 1104 WP_137222170.1 hypothetical protein -
  FCN25_RS03990 pilU 932452..933564 (-) 1113 WP_137222172.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FCN25_RS03995 pilT 933576..934613 (-) 1038 WP_137222174.1 type IV pilus twitching motility protein PilT Machinery gene
  FCN25_RS04000 - 934641..935327 (+) 687 WP_137222176.1 YggS family pyridoxal phosphate-dependent enzyme -
  FCN25_RS04005 proC 935570..936388 (+) 819 WP_137222178.1 pyrroline-5-carboxylate reductase -
  FCN25_RS04010 - 936582..937130 (+) 549 WP_137222180.1 YggT family protein -
  FCN25_RS04015 yggU 937151..937450 (+) 300 WP_137222182.1 DUF167 family protein YggU -
  FCN25_RS04020 - 937537..937998 (+) 462 WP_137222184.1 DUF4426 domain-containing protein -
  FCN25_RS04025 rdgB 938232..938885 (+) 654 WP_137222186.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38389.95 Da        Isoelectric Point: 6.9094

>NTDB_id=361003 FCN25_RS03995 WP_137222174.1 933576..934613(-) (pilT) [Shewanella sp. MEBiC00475]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAGAVFRTIPSDILSLEQLGAPDIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMSHGMQTLDQCL
QNLVNRGLITREDAMQKSSNKQTNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=361003 FCN25_RS03995 WP_137222174.1 933576..934613(-) (pilT) [Shewanella sp. MEBiC00475]
ATGGAAATCACTGAATTACTCGCCTTTAGTGTAAAACACAAAGCATCAGATCTACATCTTTCAGCGGGCGTGTCCCCGAT
GATCCGTGTTGATGGTGAGGTGAGAAAAATTAACTTACCTGCATTAGATCATCAAGGCGTTCATAGTCTTGTATATGACA
TCATGAATGATAAACAACGTAAAGACTATGAAGAACATCTTGAAATCGATTTTTCATTTGAAGTGCCTAACCTAGCACGT
TTTCGTGTTAACGCATTCAATCAGTCTCGTGGTGCTGGCGCAGTATTTCGTACTATTCCGTCAGATATTTTGTCGCTTGA
GCAGCTTGGCGCGCCAGACATTTTCAAGAAAATATCCAGTTTCCCTCGTGGCTTAGTCTTAGTTACAGGGCCGACGGGTT
CAGGTAAGTCAACCACCTTAGCTGCAATGGTAGATTACGTTAATGAAAATCGACATGATCATATTTTAACCATTGAAGAT
CCAATCGAATTCGTTCATCAGAACAAACAATGCTTAATCAACCAGCGCGAAGTTCATAAGCATACCCATAGCTTTAACGC
TGCACTGAGAAGTGCATTACGTGAAGACCCGGATGTTATTCTTGTGGGTGAAATGCGTGACCTTGAAACCATTCGTTTAG
CTATGACAGCCGCTGAAACGGGCCATTTAGTGTTTGGTACCTTGCATACCACATCAGCCGCAAAAACCATTGACCGTGTG
GTCGATGTATTTCCCGAAGGTGAAAAGAGTATGGTTCGCACCATGCTGTCTGAATCACTTCAAGCAGTTATTTCGCAAAC
ACTGATCAAAAAAATTGGTGGTGGTCGAGTTGCCGCTCACGAAATTATGATGGGTACTCCAGCGATACGAAATCTGATCC
GCGAAGATAAAGTCGCGCAAATGTATTCTGCCATTCAAACAGGTATGTCACACGGTATGCAAACCCTCGACCAATGCCTG
CAAAACTTAGTCAATCGTGGTTTAATTACACGCGAAGATGCAATGCAAAAAAGTTCGAATAAACAAACTAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.391

100

0.774
  pilT Acinetobacter baylyi ADP1

79.939

95.362

0.762
  pilT Legionella pneumophila strain Lp02

74.78

98.841

0.739
  pilT Legionella pneumophila strain ERS1305867

74.78

98.841

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.681

0.699
  pilT Vibrio cholerae strain A1552

71.513

97.681

0.699
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

42.262

97.391

0.412
  pilU Vibrio cholerae strain A1552

40.762

98.841

0.403
  pilU Acinetobacter baylyi ADP1

39.941

97.971

0.391

Multiple sequence alignment