Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FCN25_RS03990 Genome accession   NZ_CP039928
Coordinates   932452..933564 (-) Length   370 a.a.
NCBI ID   WP_137222172.1    Uniprot ID   -
Organism   Shewanella sp. MEBiC00475     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 927452..938564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FCN25_RS03965 - 928006..928566 (-) 561 WP_137222162.1 YqgE/AlgH family protein -
  FCN25_RS03970 yciH 928600..928929 (-) 330 WP_137222164.1 stress response translation initiation inhibitor YciH -
  FCN25_RS03975 hemH 929189..930202 (-) 1014 WP_137222166.1 ferrochelatase -
  FCN25_RS03980 - 930457..930990 (-) 534 WP_137222168.1 glutathione peroxidase -
  FCN25_RS03985 - 931100..932203 (-) 1104 WP_137222170.1 hypothetical protein -
  FCN25_RS03990 pilU 932452..933564 (-) 1113 WP_137222172.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FCN25_RS03995 pilT 933576..934613 (-) 1038 WP_137222174.1 type IV pilus twitching motility protein PilT Machinery gene
  FCN25_RS04000 - 934641..935327 (+) 687 WP_137222176.1 YggS family pyridoxal phosphate-dependent enzyme -
  FCN25_RS04005 proC 935570..936388 (+) 819 WP_137222178.1 pyrroline-5-carboxylate reductase -
  FCN25_RS04010 - 936582..937130 (+) 549 WP_137222180.1 YggT family protein -
  FCN25_RS04015 yggU 937151..937450 (+) 300 WP_137222182.1 DUF167 family protein YggU -
  FCN25_RS04020 - 937537..937998 (+) 462 WP_137222184.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41137.12 Da        Isoelectric Point: 6.0925

>NTDB_id=361002 FCN25_RS03990 WP_137222172.1 932452..933564(-) (pilU) [Shewanella sp. MEBiC00475]
MDVRPFLNIMVEKKASDLFVTAGFPPSAKIDGELRQLSENRLTPEQSLVFVESLMSDVQKKEFHETSECNFAFAAKDLGR
FRVSAFWQRESAGCVMRRIETQIPEVDDLKLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLIPKSDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMGKSREQGMQTFDQA
LLDLYVEGEISYADALHHADSPNDLRLMIKLQSNETTSSGIMEGITFDKD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=361002 FCN25_RS03990 WP_137222172.1 932452..933564(-) (pilU) [Shewanella sp. MEBiC00475]
ATGGATGTTCGTCCATTTTTAAATATCATGGTTGAGAAAAAGGCGTCTGATTTATTTGTCACGGCAGGTTTTCCACCCAG
TGCTAAAATTGATGGTGAGTTACGTCAACTTTCTGAAAATCGATTAACACCAGAGCAATCGTTGGTGTTTGTTGAGTCCT
TGATGAGCGATGTGCAAAAAAAAGAGTTTCACGAAACCAGTGAATGTAACTTTGCTTTTGCGGCAAAAGATTTAGGGCGC
TTTCGTGTCAGTGCATTTTGGCAACGTGAATCAGCCGGCTGCGTGATGCGTCGTATTGAAACTCAAATTCCAGAAGTCGA
TGACTTAAAACTGCCTGCTATTCTAAAAGACCTGGTCATGAGTAAACGTGGTCTTATCATTATGGTTGGCGGAACGGGTA
CCGGTAAGTCAACATCGTTAGCTGCATTAGTGGGTTACCGAAACGCGCATGCCCGTGGACATATCCTCACCATCGAAGAC
CCAGTTGAATTTGTTCATAACCATCGTAAAAGTATTATTACTCAACGAGAAGTGGGTATTGATACTGAATCATTTGATGC
CGCACTGAAAAGCTCGTTACGCCAGGCGCCAGATGTGATTCTGATTGGTGAAATCCGCACGCAAGAAACCATGGAGTTTG
CATTGTCATTTGCTGAAACGGGTCATTTATGCATGGCGACCTTGCATGCTAACAATGCTAACCAAGCACTTGATCGCATT
ATGCATTTAGTGCCAGAAAGTAAGCATAATCAACTCTTATTCGATTTGTCATTGAACTTACGCGGCATTGTCGCGCAACA
GCTTATACCTAAATCAGACGGAACCGGCCGTCGTGCAGCCATTGAAGTGTTAATTAATACTCCTCGCGTGGCCAGTTTGA
TTGCTAAAAATGAGTTGCATTTACTCAAAGAAACCATGGGCAAGTCACGCGAACAAGGCATGCAAACGTTCGACCAAGCT
TTGCTAGATTTATATGTAGAGGGGGAAATTAGTTACGCCGATGCATTACATCATGCCGACTCACCTAATGATTTACGTTT
AATGATTAAATTACAGAGTAACGAAACAACCTCTTCGGGGATAATGGAAGGCATTACTTTCGATAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.647

100

0.608
  pilU Acinetobacter baylyi ADP1

60.227

95.135

0.573
  pilU Vibrio cholerae strain A1552

57.065

99.459

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.159

93.243

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Acinetobacter baumannii strain A118

39.766

92.432

0.368
  pilT Acinetobacter baumannii D1279779

39.766

92.432

0.368
  pilT Acinetobacter nosocomialis M2

39.766

92.432

0.368
  pilT Acinetobacter baylyi ADP1

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362

Multiple sequence alignment