Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FBQ74_RS04285 Genome accession   NZ_CP039852
Coordinates   982065..983090 (+) Length   341 a.a.
NCBI ID   WP_139755491.1    Uniprot ID   A0A5B7YAP8
Organism   Salinimonas iocasae strain KX18D6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 977065..988090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBQ74_RS04255 (FBQ74_04255) yaaA 978184..978960 (+) 777 WP_139755485.1 peroxide stress protein YaaA -
  FBQ74_RS04260 (FBQ74_04260) - 979013..979237 (-) 225 WP_139755486.1 hypothetical protein -
  FBQ74_RS04265 (FBQ74_04265) - 979456..979896 (-) 441 WP_139755487.1 DUF4426 domain-containing protein -
  FBQ74_RS04270 (FBQ74_04270) - 979923..980465 (-) 543 WP_139755488.1 YggT family protein -
  FBQ74_RS04275 (FBQ74_04275) proC 980486..981307 (-) 822 WP_139755489.1 pyrroline-5-carboxylate reductase -
  FBQ74_RS04280 (FBQ74_04280) - 981346..982035 (-) 690 WP_139755490.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBQ74_RS04285 (FBQ74_04285) pilT 982065..983090 (+) 1026 WP_139755491.1 type IV pilus twitching motility protein PilT Machinery gene
  FBQ74_RS04290 (FBQ74_04290) pilU 983105..984211 (+) 1107 WP_139755492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FBQ74_RS04295 (FBQ74_04295) - 984214..984579 (+) 366 WP_139755493.1 DUF2007 domain-containing protein -
  FBQ74_RS04300 (FBQ74_04300) ruvX 984582..985013 (-) 432 WP_139755494.1 Holliday junction resolvase RuvX -
  FBQ74_RS04305 (FBQ74_04305) - 985006..985572 (-) 567 WP_139755495.1 YqgE/AlgH family protein -
  FBQ74_RS04310 (FBQ74_04310) gshB 985587..986537 (-) 951 WP_139755496.1 glutathione synthase -
  FBQ74_RS04315 (FBQ74_04315) - 986619..987347 (-) 729 WP_139755497.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 37958.56 Da        Isoelectric Point: 6.3482

>NTDB_id=360770 FBQ74_RS04285 WP_139755491.1 982065..983090(+) (pilT) [Salinimonas iocasae strain KX18D6]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGQMRRLNIPELDHKQVHSLIYEIMNDAQRKEYEENLEVDFSFEVKDLSR
FRVNAFVQNRGAAAVLRTIPSRVLSLDELGAPDIFKQIIDQPTGLVLVTGATGSGKSTTLAAMIDHINNFKRDHILTIED
PIEFVHENKLSLINQREVHRDTRSFNNALRSALREDPDVILVGELRDLETIRLAMSAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKSMIRSMLSESLRAVISQTLLQKAGGGRIAAHEIMLGVPAIRNLIREDKVPQMYSVIQTGQAQGMQTMEQCL
QRLVAKGLVSQQDASAKAIDK

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=360770 FBQ74_RS04285 WP_139755491.1 982065..983090(+) (pilT) [Salinimonas iocasae strain KX18D6]
GTGGATATTACCGAACTTTTAGCGTTCAGTGTTAAGAATAACGCATCAGACTTGCACCTGTCAGCGGGCTTGCCGCCGAT
TATTCGAGTTGACGGTCAGATGCGCCGCCTCAATATCCCCGAGCTGGATCATAAACAGGTTCACAGCCTGATTTATGAAA
TCATGAATGATGCCCAGCGTAAGGAGTACGAAGAAAATCTTGAGGTCGATTTTTCTTTTGAGGTAAAAGATTTATCGCGC
TTCAGGGTCAATGCATTTGTTCAGAACCGGGGCGCGGCGGCCGTACTACGGACCATCCCCTCACGCGTTTTATCGCTGGA
TGAACTTGGCGCACCGGACATTTTCAAACAGATAATCGACCAGCCTACTGGTCTGGTGCTTGTAACCGGTGCCACGGGGT
CGGGTAAAAGTACTACGCTGGCGGCGATGATTGATCACATCAATAATTTTAAACGTGATCATATTCTTACTATAGAAGAT
CCCATCGAATTTGTTCACGAAAACAAACTCAGCCTTATCAACCAGAGGGAAGTGCACCGCGATACCCGTAGCTTTAATAA
CGCGCTGCGCTCTGCATTGCGTGAAGATCCCGATGTGATACTGGTGGGTGAATTGCGGGACCTTGAGACCATTCGCCTGG
CGATGTCCGCTGCGGAAACCGGTCACCTTGTTTTCGGCACATTGCATACTAACTCTGCGCCTAAAACTATTGACCGTATA
ATTGATGTGTTTCCCGCTGAAGAAAAATCTATGATTCGCTCTATGCTGTCTGAATCGCTGCGTGCCGTTATCTCTCAAAC
ACTGTTACAAAAAGCCGGGGGCGGACGAATCGCTGCGCACGAAATTATGCTGGGCGTACCGGCTATCCGAAACCTTATTC
GTGAAGACAAGGTGCCGCAAATGTATTCGGTTATTCAGACCGGTCAGGCGCAGGGAATGCAAACCATGGAACAGTGTCTG
CAACGACTGGTTGCCAAGGGGCTGGTATCACAGCAGGATGCCAGTGCCAAAGCTATCGATAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7YAP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

74.556

99.12

0.739
  pilT Pseudomonas aeruginosa PAK

74.556

99.12

0.739
  pilT Acinetobacter baylyi ADP1

74.556

99.12

0.739
  pilT Acinetobacter baumannii D1279779

73.669

99.12

0.73
  pilT Acinetobacter baumannii strain A118

73.669

99.12

0.73
  pilT Acinetobacter nosocomialis M2

73.373

99.12

0.727
  pilT Legionella pneumophila strain Lp02

70.968

100

0.71
  pilT Legionella pneumophila strain ERS1305867

70.968

100

0.71
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.326

98.827

0.695
  pilT Vibrio cholerae strain A1552

70.326

98.827

0.695
  pilT Neisseria meningitidis 8013

66.667

99.413

0.663
  pilT Neisseria gonorrhoeae MS11

66.372

99.413

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.372

100

0.534
  pilU Vibrio cholerae strain A1552

42.09

98.24

0.413
  pilU Acinetobacter baylyi ADP1

40.588

99.707

0.405
  pilU Pseudomonas stutzeri DSM 10701

40

98.24

0.393

Multiple sequence alignment