Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FBQ74_RS04290 Genome accession   NZ_CP039852
Coordinates   983105..984211 (+) Length   368 a.a.
NCBI ID   WP_139755492.1    Uniprot ID   A0A5B7YBR6
Organism   Salinimonas iocasae strain KX18D6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 978105..989211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FBQ74_RS04255 (FBQ74_04255) yaaA 978184..978960 (+) 777 WP_139755485.1 peroxide stress protein YaaA -
  FBQ74_RS04260 (FBQ74_04260) - 979013..979237 (-) 225 WP_139755486.1 hypothetical protein -
  FBQ74_RS04265 (FBQ74_04265) - 979456..979896 (-) 441 WP_139755487.1 DUF4426 domain-containing protein -
  FBQ74_RS04270 (FBQ74_04270) - 979923..980465 (-) 543 WP_139755488.1 YggT family protein -
  FBQ74_RS04275 (FBQ74_04275) proC 980486..981307 (-) 822 WP_139755489.1 pyrroline-5-carboxylate reductase -
  FBQ74_RS04280 (FBQ74_04280) - 981346..982035 (-) 690 WP_139755490.1 YggS family pyridoxal phosphate-dependent enzyme -
  FBQ74_RS04285 (FBQ74_04285) pilT 982065..983090 (+) 1026 WP_139755491.1 type IV pilus twitching motility protein PilT Machinery gene
  FBQ74_RS04290 (FBQ74_04290) pilU 983105..984211 (+) 1107 WP_139755492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FBQ74_RS04295 (FBQ74_04295) - 984214..984579 (+) 366 WP_139755493.1 DUF2007 domain-containing protein -
  FBQ74_RS04300 (FBQ74_04300) ruvX 984582..985013 (-) 432 WP_139755494.1 Holliday junction resolvase RuvX -
  FBQ74_RS04305 (FBQ74_04305) - 985006..985572 (-) 567 WP_139755495.1 YqgE/AlgH family protein -
  FBQ74_RS04310 (FBQ74_04310) gshB 985587..986537 (-) 951 WP_139755496.1 glutathione synthase -
  FBQ74_RS04315 (FBQ74_04315) - 986619..987347 (-) 729 WP_139755497.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FBQ74_RS04325 (FBQ74_04325) - 988073..988972 (+) 900 WP_139755499.1 mechanosensitive ion channel family protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40643.26 Da        Isoelectric Point: 4.8026

>NTDB_id=360771 FBQ74_RS04290 WP_139755492.1 983105..984211(+) (pilU) [Salinimonas iocasae strain KX18D6]
MLTDYLQQATEQGASDIFITTGFPVSAKINGQLTPLDDHTLSPEDAKSLVHSVMEQRYIDELDTTKEANFAVGLDGIGRF
RCSAFWQRDSVGMVVRRIVTDIPKVDDLGLPPILKDVIMSKRGLILMVGATGTGKSTSLAALIDHRNHNSRGHILTIEDP
IEFVHQHGSCVVTQREVGVDTQSFDEALKSSLRQAPDVILLGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPRELHDKLLFDLSLNLRAIVAQQLVPNKDGGRVAAIEILLNTPFIRELIQNDRIGELKEAMKKSKEAGMQTFDMALY
ELYQNGDIELEQALHHADSPNDLRLMIKLDSDSGSSLGSLSDVSIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=360771 FBQ74_RS04290 WP_139755492.1 983105..984211(+) (pilU) [Salinimonas iocasae strain KX18D6]
GTGTTAACTGATTACTTGCAACAGGCCACCGAGCAGGGGGCGTCGGATATTTTTATCACCACTGGTTTTCCGGTCAGTGC
AAAAATTAATGGCCAGCTTACGCCTCTTGATGATCACACGCTGTCTCCTGAAGATGCTAAAAGCCTTGTTCACAGTGTGA
TGGAGCAGCGCTACATTGATGAGCTGGATACGACGAAGGAGGCCAATTTCGCAGTCGGTTTGGATGGTATCGGCCGGTTC
CGGTGTAGTGCTTTCTGGCAGCGTGATTCCGTAGGCATGGTGGTACGCCGGATTGTGACCGATATACCCAAGGTAGACGA
CCTGGGCCTGCCGCCAATTCTTAAAGATGTGATTATGAGTAAGCGAGGCCTTATTCTGATGGTCGGTGCAACAGGTACCG
GTAAATCAACGTCACTGGCTGCACTTATCGATCACAGAAATCATAACTCTCGTGGTCATATCCTGACTATCGAGGACCCG
ATAGAGTTTGTCCATCAGCATGGTAGTTGCGTGGTGACCCAGCGGGAGGTTGGTGTCGATACCCAGTCATTTGACGAAGC
GCTTAAAAGCTCACTGCGTCAGGCGCCGGATGTTATTCTTTTAGGCGAAATCCGTTCGATGGAAACCATGGAATATGCGA
TGTCGTTTGCCGATACCGGTCACCTGTGTGTGGCAACGCTGCACGCCAACAATGCTAACCAGGCGATTGAGCGGATCATG
CACCTGGCTCCGCGTGAACTACACGATAAGCTATTGTTCGATTTAAGCCTTAATTTGCGCGCTATCGTGGCTCAGCAGCT
TGTACCCAATAAGGACGGCGGGCGCGTTGCTGCTATCGAAATTCTGCTCAACACACCGTTTATCCGGGAACTGATTCAGA
ACGACAGAATTGGCGAGCTCAAAGAAGCGATGAAAAAGAGTAAAGAGGCAGGCATGCAGACGTTTGATATGGCGCTGTAT
GAGCTTTATCAAAACGGTGATATTGAGCTTGAGCAGGCACTGCATCATGCTGACTCACCAAATGATCTGCGTCTGATGAT
CAAGCTTGATTCAGACAGTGGCTCAAGCCTTGGTTCGTTAAGTGATGTTTCTATCGATATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B7YBR6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.659

97.283

0.571
  pilU Vibrio cholerae strain A1552

54.348

100

0.543
  pilU Acinetobacter baylyi ADP1

53.973

99.185

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.274

98.098

0.405
  pilT Legionella pneumophila strain Lp02

41.369

91.304

0.378
  pilT Legionella pneumophila strain ERS1305867

41.369

91.304

0.378

Multiple sequence alignment