Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FAY30_RS17860 Genome accession   NZ_CP039727
Coordinates   3664585..3665634 (-) Length   349 a.a.
NCBI ID   WP_149871145.1    Uniprot ID   -
Organism   Bacillus sp. S3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3659585..3670634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAY30_RS17835 (FAY30_17835) - 3660116..3660997 (-) 882 WP_149871140.1 PilN domain-containing protein -
  FAY30_RS17840 (FAY30_17840) pilM 3660997..3661992 (-) 996 WP_149871141.1 type IV pilus biogenesis protein PilM -
  FAY30_RS17845 (FAY30_17845) comC 3662009..3662767 (-) 759 WP_149871142.1 prepilin peptidase Machinery gene
  FAY30_RS17850 (FAY30_17850) - 3662767..3663237 (-) 471 WP_149871143.1 type II secretion system protein -
  FAY30_RS17855 (FAY30_17855) - 3663378..3664592 (-) 1215 WP_149871144.1 type II secretion system F family protein -
  FAY30_RS17860 (FAY30_17860) pilT 3664585..3665634 (-) 1050 WP_149871145.1 type IV pilus twitching motility protein PilT Machinery gene
  FAY30_RS17865 (FAY30_17865) - 3665647..3667308 (-) 1662 WP_149871146.1 GspE/PulE family protein -
  FAY30_RS17870 (FAY30_17870) - 3667324..3668655 (-) 1332 WP_149871147.1 VanW family protein -
  FAY30_RS17875 (FAY30_17875) - 3668680..3669324 (-) 645 WP_149871148.1 hypothetical protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38750.72 Da        Isoelectric Point: 9.5889

>NTDB_id=360471 FAY30_RS17860 WP_149871145.1 3664585..3665634(-) (pilT) [Bacillus sp. S3]
MKEKIDTILRAAIEYKASDVHLTVGVPPVLRINGDLRRYGSDKLMPDDTEGMAKAIIPDDLWERFKSKGELDFSYGVPGV
SRFRVNAYHQRKSVSIALRTVASKTPTIEELDLPPVISKLVQKPQGLILVTGPTGSGKSTTLASMIDYMNRTMRKHIITL
EDPIEYLHRHGTSIIDQREVGFDTNNYASGLKAALRQDPDVILVGEMRDLETMSIAITAAETGHLVLGTLHTSSAPTTIN
RIIDVFPSGQQPQIRIQLASVLVGVISQRLFPMADKKGRKAATEILVNNAAIANLIRNEKIHQIQSTMQTSRAQGMHTLE
MSIRDLIDRNVIQRDAASQYLQEKMMMNG

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=360471 FAY30_RS17860 WP_149871145.1 3664585..3665634(-) (pilT) [Bacillus sp. S3]
ATGAAAGAAAAAATTGACACAATCTTACGAGCGGCTATTGAATACAAAGCATCTGATGTGCATTTAACGGTTGGTGTTCC
CCCGGTGTTGCGAATTAATGGTGACCTTAGACGGTATGGATCCGATAAATTAATGCCCGACGACACTGAGGGAATGGCGA
AAGCAATCATCCCAGATGATTTGTGGGAAAGATTTAAGAGTAAAGGGGAGCTTGATTTTTCCTATGGTGTACCTGGTGTG
TCACGTTTTCGTGTCAATGCCTATCATCAAAGGAAAAGTGTTTCGATTGCACTGAGGACGGTTGCATCAAAAACTCCGAC
AATTGAGGAGTTGGATTTACCCCCGGTCATTTCTAAGCTCGTTCAGAAGCCGCAAGGTCTGATTCTTGTTACCGGGCCAA
CAGGTAGCGGTAAATCGACTACATTAGCCTCAATGATTGATTACATGAACCGAACAATGCGAAAGCATATCATTACATTA
GAGGATCCAATCGAGTATCTTCATAGACATGGGACTTCGATTATTGATCAAAGGGAAGTCGGGTTTGATACAAATAACTA
TGCCAGCGGCTTAAAGGCGGCATTAAGGCAAGACCCTGATGTCATTCTTGTTGGGGAGATGCGTGACTTAGAAACGATGT
CTATTGCGATTACAGCGGCAGAAACAGGCCATCTTGTATTAGGGACACTTCATACATCGAGTGCACCAACAACGATTAAC
CGGATCATCGATGTATTCCCATCAGGGCAGCAGCCGCAAATTCGGATCCAGTTGGCTTCGGTGTTAGTGGGGGTTATTTC
TCAACGGTTATTTCCTATGGCTGATAAAAAGGGCAGGAAAGCAGCAACAGAAATACTGGTGAATAACGCTGCCATCGCCA
ACTTGATTAGAAATGAAAAAATCCACCAAATTCAAAGCACAATGCAGACGTCCCGGGCCCAGGGAATGCATACGCTGGAG
ATGAGCATAAGAGATTTAATTGATCGAAATGTGATCCAAAGAGATGCAGCGTCACAATATTTGCAAGAAAAGATGATGAT
GAATGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.755

94.842

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.093

98.567

0.464
  pilT Vibrio cholerae strain A1552

47.093

98.567

0.464
  pilT Acinetobacter baumannii strain A118

46.903

97.135

0.456
  pilT Acinetobacter baumannii D1279779

46.903

97.135

0.456
  pilT Acinetobacter nosocomialis M2

46.903

97.135

0.456
  pilT Pseudomonas aeruginosa PAK

46.866

95.989

0.45
  pilT Pseudomonas stutzeri DSM 10701

46.567

95.989

0.447
  pilT Neisseria gonorrhoeae MS11

45.217

98.854

0.447
  pilT Neisseria meningitidis 8013

44.928

98.854

0.444
  pilT Legionella pneumophila strain Lp02

45.075

95.989

0.433
  pilT Legionella pneumophila strain ERS1305867

45.075

95.989

0.433
  pilT Acinetobacter baylyi ADP1

45.897

94.269

0.433
  pilU Vibrio cholerae strain A1552

41.018

95.702

0.393
  pilU Acinetobacter baylyi ADP1

40.122

94.269

0.378
  pilU Pseudomonas stutzeri DSM 10701

40.248

92.55

0.372

Multiple sequence alignment